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Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain
Infection with the deadly rabies virus (RABV) leads to alteration of cellular gene expression. The RABV, similar to other neurodegenerative diseases may be implicated in neuronal death due to an imbalance in Ca(2+) homeostasis. Parvalbumin (PV) and Secretagogin (Scgn), two members of the Calcium-Bin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008021/ https://www.ncbi.nlm.nih.gov/pubmed/33783673 http://dx.doi.org/10.1007/s11011-021-00717-4 |
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author | Kanu, Brenda Kia, Grace S. N. Aimola, Idowu A. Korie, George C. Tekki, Ishaya S. |
author_facet | Kanu, Brenda Kia, Grace S. N. Aimola, Idowu A. Korie, George C. Tekki, Ishaya S. |
author_sort | Kanu, Brenda |
collection | PubMed |
description | Infection with the deadly rabies virus (RABV) leads to alteration of cellular gene expression. The RABV, similar to other neurodegenerative diseases may be implicated in neuronal death due to an imbalance in Ca(2+) homeostasis. Parvalbumin (PV) and Secretagogin (Scgn), two members of the Calcium-Binding Proteins (CBPs) are useful neuronal markers responsible for calcium regulation and buffering with possible protective roles against infections. This study investigated whether infection with rabies virus causes variance in expression levels of PV and Scgn using the Challenge virus standard (CVS) and Nigerian Street Rabies virus (SRV) strains. Forty-eight, 4-week-old BALB/c mice strains were divided into two test groups and challenged with Rabies virus (RABV) infection and one control group. The presence of RABV antigen was verified by direct fluorescent antibody test (DFAT) and real-time quantitative PCR (qRT-PCR) was used to assess PV and Scgn gene expression. Infection with both virus strains resulted in significant (p < 0.05) increases in expression during early infection. Mid-infection phase caused reduced expression for both genes. However, as infection progressed to the terminal phase, a lower increase in expression was measured. Gene expression and viral load correlation indicated no positive relationship. Neurons with these CBPs may have a greater capacity to buffer calcium and be more resistant to degenerative changes caused by RABV. This implies that, when PV and Scgn expression levels are kept adequately high, the integrity of neurons may be maintained and degeneration caused by RABV infection may be prevented or stopped, hence, these are possible constituents of effective rabies therapy. |
format | Online Article Text |
id | pubmed-8008021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-80080212021-03-30 Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain Kanu, Brenda Kia, Grace S. N. Aimola, Idowu A. Korie, George C. Tekki, Ishaya S. Metab Brain Dis Original Article Infection with the deadly rabies virus (RABV) leads to alteration of cellular gene expression. The RABV, similar to other neurodegenerative diseases may be implicated in neuronal death due to an imbalance in Ca(2+) homeostasis. Parvalbumin (PV) and Secretagogin (Scgn), two members of the Calcium-Binding Proteins (CBPs) are useful neuronal markers responsible for calcium regulation and buffering with possible protective roles against infections. This study investigated whether infection with rabies virus causes variance in expression levels of PV and Scgn using the Challenge virus standard (CVS) and Nigerian Street Rabies virus (SRV) strains. Forty-eight, 4-week-old BALB/c mice strains were divided into two test groups and challenged with Rabies virus (RABV) infection and one control group. The presence of RABV antigen was verified by direct fluorescent antibody test (DFAT) and real-time quantitative PCR (qRT-PCR) was used to assess PV and Scgn gene expression. Infection with both virus strains resulted in significant (p < 0.05) increases in expression during early infection. Mid-infection phase caused reduced expression for both genes. However, as infection progressed to the terminal phase, a lower increase in expression was measured. Gene expression and viral load correlation indicated no positive relationship. Neurons with these CBPs may have a greater capacity to buffer calcium and be more resistant to degenerative changes caused by RABV. This implies that, when PV and Scgn expression levels are kept adequately high, the integrity of neurons may be maintained and degeneration caused by RABV infection may be prevented or stopped, hence, these are possible constituents of effective rabies therapy. Springer US 2021-03-30 2021 /pmc/articles/PMC8008021/ /pubmed/33783673 http://dx.doi.org/10.1007/s11011-021-00717-4 Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Kanu, Brenda Kia, Grace S. N. Aimola, Idowu A. Korie, George C. Tekki, Ishaya S. Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
title | Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
title_full | Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
title_fullStr | Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
title_full_unstemmed | Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
title_short | Rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
title_sort | rabies virus infection is associated with alterations in the expression of parvalbumin and secretagogin in mice brain |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008021/ https://www.ncbi.nlm.nih.gov/pubmed/33783673 http://dx.doi.org/10.1007/s11011-021-00717-4 |
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