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Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes
INTRODUCTION: Melanocytes are engaged in synthesis, transport, and release of pigments at the epidermal-melanin units in response to the finely regulated melanogenic pathway. A multifaceted combination of both intrinsic and extrinsic factors – from endocrine and paracrine dynamics to exogenous stimu...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008161/ https://www.ncbi.nlm.nih.gov/pubmed/33790611 http://dx.doi.org/10.2147/CCID.S299569 |
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author | Ferraro, Maria Grazia Piccolo, Marialuisa Pezzella, Alessandro Guerra, Fabrizia Maione, Francesco Tenore, Gian Carlo Santamaria, Rita Irace, Carlo Novellino, Ettore |
author_facet | Ferraro, Maria Grazia Piccolo, Marialuisa Pezzella, Alessandro Guerra, Fabrizia Maione, Francesco Tenore, Gian Carlo Santamaria, Rita Irace, Carlo Novellino, Ettore |
author_sort | Ferraro, Maria Grazia |
collection | PubMed |
description | INTRODUCTION: Melanocytes are engaged in synthesis, transport, and release of pigments at the epidermal-melanin units in response to the finely regulated melanogenic pathway. A multifaceted combination of both intrinsic and extrinsic factors – from endocrine and paracrine dynamics to exogenous stimuli such as sunlight and xenobiotics – modulates expression and activity of proteins involved in pigmentation, including the rate-limiting enzyme tyrosinase. As well as playing critical physiological functions comprising skin photoprotection, melanins define hair and skin pigmentation which in turn have impacted considerably to human social communication since time immemorial. Additionally, numerous skin diseases based on pigmentation alterations can have serious public influence. While several melanogenesis inhibitors are already available, the number of melanin activators and tyrosinase stimulators as drug-like agents is still limited. METHODS: To explore the biological effects of an Annurca Apple-based nutraceutical preparation (AMS) on melanin production, experiments in cellular models of human skin were performed. Both primary cultures and co-cultures of epidermal melanocytes (HEMa) and follicular keratinocytes (HHFK) were used. RESULTS: We show that AMS, by now branded for its cutaneous beneficial effects, induces in total biocompatibility a significant promelanogenic effect in human primary melanocytes. In line, we found melanin cytosolic accumulation consistent with tyrosinase up-regulation. CONCLUSION: Disposal of skin pigmenting agents would be attractive for the treatment of hypopigmentation disorders, to postpone skin photoaging or simply for fashion, so that discovery and development of melanogenesis stimulators, especially from natural sources, is nowadays a dynamic area of research. |
format | Online Article Text |
id | pubmed-8008161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-80081612021-03-30 Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes Ferraro, Maria Grazia Piccolo, Marialuisa Pezzella, Alessandro Guerra, Fabrizia Maione, Francesco Tenore, Gian Carlo Santamaria, Rita Irace, Carlo Novellino, Ettore Clin Cosmet Investig Dermatol Original Research INTRODUCTION: Melanocytes are engaged in synthesis, transport, and release of pigments at the epidermal-melanin units in response to the finely regulated melanogenic pathway. A multifaceted combination of both intrinsic and extrinsic factors – from endocrine and paracrine dynamics to exogenous stimuli such as sunlight and xenobiotics – modulates expression and activity of proteins involved in pigmentation, including the rate-limiting enzyme tyrosinase. As well as playing critical physiological functions comprising skin photoprotection, melanins define hair and skin pigmentation which in turn have impacted considerably to human social communication since time immemorial. Additionally, numerous skin diseases based on pigmentation alterations can have serious public influence. While several melanogenesis inhibitors are already available, the number of melanin activators and tyrosinase stimulators as drug-like agents is still limited. METHODS: To explore the biological effects of an Annurca Apple-based nutraceutical preparation (AMS) on melanin production, experiments in cellular models of human skin were performed. Both primary cultures and co-cultures of epidermal melanocytes (HEMa) and follicular keratinocytes (HHFK) were used. RESULTS: We show that AMS, by now branded for its cutaneous beneficial effects, induces in total biocompatibility a significant promelanogenic effect in human primary melanocytes. In line, we found melanin cytosolic accumulation consistent with tyrosinase up-regulation. CONCLUSION: Disposal of skin pigmenting agents would be attractive for the treatment of hypopigmentation disorders, to postpone skin photoaging or simply for fashion, so that discovery and development of melanogenesis stimulators, especially from natural sources, is nowadays a dynamic area of research. Dove 2021-03-25 /pmc/articles/PMC8008161/ /pubmed/33790611 http://dx.doi.org/10.2147/CCID.S299569 Text en © 2021 Ferraro et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Ferraro, Maria Grazia Piccolo, Marialuisa Pezzella, Alessandro Guerra, Fabrizia Maione, Francesco Tenore, Gian Carlo Santamaria, Rita Irace, Carlo Novellino, Ettore Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes |
title | Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes |
title_full | Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes |
title_fullStr | Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes |
title_full_unstemmed | Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes |
title_short | Promelanogenic Effects by an Annurca Apple-Based Natural Formulation in Human Primary Melanocytes |
title_sort | promelanogenic effects by an annurca apple-based natural formulation in human primary melanocytes |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008161/ https://www.ncbi.nlm.nih.gov/pubmed/33790611 http://dx.doi.org/10.2147/CCID.S299569 |
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