Atg27p localization is clathrin- and Ent3p/5p-dependent

The autophagy-related protein Atg27p has been previously shown to localize to the autophagy-specific pre-autophagosomal structure (PAS) as well as to several organelles, including the late Golgi, the vacuolar membrane, and the endosome. Given that Atg27p localization to the vacuolar membrane in part...

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Detalles Bibliográficos
Autores principales: Segarra, Verónica A., Sharma, Anupam, Lemmon, Sandra K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008256/
https://www.ncbi.nlm.nih.gov/pubmed/33817565
http://dx.doi.org/10.17912/micropub.biology.000381
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author Segarra, Verónica A.
Sharma, Anupam
Lemmon, Sandra K.
author_facet Segarra, Verónica A.
Sharma, Anupam
Lemmon, Sandra K.
author_sort Segarra, Verónica A.
collection PubMed
description The autophagy-related protein Atg27p has been previously shown to localize to the autophagy-specific pre-autophagosomal structure (PAS) as well as to several organelles, including the late Golgi, the vacuolar membrane, and the endosome. Given that Atg27p localization to the vacuolar membrane in particular has been shown to be dependent on both its C-terminal tyrosine sorting motif and the AP-3 adaptor, and that Atg27p can be found in clathrin-coated vesicles, we set out to determine whether Atg27p localization inside cells is dependent on clathrin or on any of its cargo adaptors. We report that Atg27p localization is clathrin- and Ent3p/5p-dependent.
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spelling pubmed-80082562021-04-01 Atg27p localization is clathrin- and Ent3p/5p-dependent Segarra, Verónica A. Sharma, Anupam Lemmon, Sandra K. MicroPubl Biol New Finding The autophagy-related protein Atg27p has been previously shown to localize to the autophagy-specific pre-autophagosomal structure (PAS) as well as to several organelles, including the late Golgi, the vacuolar membrane, and the endosome. Given that Atg27p localization to the vacuolar membrane in particular has been shown to be dependent on both its C-terminal tyrosine sorting motif and the AP-3 adaptor, and that Atg27p can be found in clathrin-coated vesicles, we set out to determine whether Atg27p localization inside cells is dependent on clathrin or on any of its cargo adaptors. We report that Atg27p localization is clathrin- and Ent3p/5p-dependent. Caltech Library 2021-03-29 /pmc/articles/PMC8008256/ /pubmed/33817565 http://dx.doi.org/10.17912/micropub.biology.000381 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Segarra, Verónica A.
Sharma, Anupam
Lemmon, Sandra K.
Atg27p localization is clathrin- and Ent3p/5p-dependent
title Atg27p localization is clathrin- and Ent3p/5p-dependent
title_full Atg27p localization is clathrin- and Ent3p/5p-dependent
title_fullStr Atg27p localization is clathrin- and Ent3p/5p-dependent
title_full_unstemmed Atg27p localization is clathrin- and Ent3p/5p-dependent
title_short Atg27p localization is clathrin- and Ent3p/5p-dependent
title_sort atg27p localization is clathrin- and ent3p/5p-dependent
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008256/
https://www.ncbi.nlm.nih.gov/pubmed/33817565
http://dx.doi.org/10.17912/micropub.biology.000381
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