Cargando…
Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques
BACKGROUND: In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Various plant tissue culture media, planting systems and growth factors were evalu...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008589/ https://www.ncbi.nlm.nih.gov/pubmed/33781291 http://dx.doi.org/10.1186/s13007-021-00724-7 |
_version_ | 1783672718635302912 |
---|---|
author | Mirzaei, Leila Yadollahi, Abbas Kermani, Maryam Jafarkhani Naderpour, Masoud Zeinanloo, Ali Asghar Farsi, Maryam Davoodi, Dariush |
author_facet | Mirzaei, Leila Yadollahi, Abbas Kermani, Maryam Jafarkhani Naderpour, Masoud Zeinanloo, Ali Asghar Farsi, Maryam Davoodi, Dariush |
author_sort | Mirzaei, Leila |
collection | PubMed |
description | BACKGROUND: In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Various plant tissue culture media, planting systems and growth factors were evaluated in two promissing Iranian olive cultivars ˈAminˈ and ˈMeshkatˈ and the commercial Spanish cultivar ˈArbequinaˈ. RESULTS: The results showed that cultivars have their specific optimal media, i.e. ˈAminˈ in the MS with 4 mg/L zeatin, ˈArbequinaˈ in the OM with 1 mg/L zeatin, and ˈMeshkatˈ in the OM and MS with 2 mg/L zeatin, which produced significantly a higher number of axillary shoots than other media. The results also indicated a significant improvement in the growth indices of ˈAminˈ (number of axillary shoots) when cultured using periodical mini bioreactor (PMB) in the VS medium. In comparison with VS, OM did not reveal any significant differences when both culturing systems (PMB and semi-solid media (SSM)) were used. Regarding the effect of carbon source and light intensity, mannitol and 2000 cd sr m(−2) greatly enhanced ˈArbequinaˈ growth indices (main shoot length and growth quality). The results of genetic stability of callus induced shoots (CIS) and meristem induced shoots (MIS) revealed that 2C DNA value assessed by partec flow cytometery (FCM) had 0.01, 0.03 and 0.08 pg discrepencies in ˈAminˈ, ˈArbequinaˈ and ˈMeshkatˈ, repectively. The Amplified Fragment Length Polymorphism (AFLP) results also indicated that the cultivars were classified regardless of the micropropagation origin (CIS or MIS), except for ˈArbequinaˈ. The AFLP findings showed that ˈArbequinaˈ had the highest dispersal (7–38%) in CIS and MIS, while the Iranian cultivar of ˈMeshkatˈ (5–9%) had the highest stability. CONCLUSIONS: This study indicated the importance of in vitro growth parameters for improving the micropropagation indices of olive cultivars. It showed that optimized protocols (OM, PMB, zeatin, mannitol and 2000 cd sr m(−2)) co-produced larger calli resulting in indirect organogenesis. Based on FCM and AFLP analysis, it can be concluded that true-to-typeness of micropropagated olive was cultivar-dependent. |
format | Online Article Text |
id | pubmed-8008589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80085892021-03-30 Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques Mirzaei, Leila Yadollahi, Abbas Kermani, Maryam Jafarkhani Naderpour, Masoud Zeinanloo, Ali Asghar Farsi, Maryam Davoodi, Dariush Plant Methods Research BACKGROUND: In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Various plant tissue culture media, planting systems and growth factors were evaluated in two promissing Iranian olive cultivars ˈAminˈ and ˈMeshkatˈ and the commercial Spanish cultivar ˈArbequinaˈ. RESULTS: The results showed that cultivars have their specific optimal media, i.e. ˈAminˈ in the MS with 4 mg/L zeatin, ˈArbequinaˈ in the OM with 1 mg/L zeatin, and ˈMeshkatˈ in the OM and MS with 2 mg/L zeatin, which produced significantly a higher number of axillary shoots than other media. The results also indicated a significant improvement in the growth indices of ˈAminˈ (number of axillary shoots) when cultured using periodical mini bioreactor (PMB) in the VS medium. In comparison with VS, OM did not reveal any significant differences when both culturing systems (PMB and semi-solid media (SSM)) were used. Regarding the effect of carbon source and light intensity, mannitol and 2000 cd sr m(−2) greatly enhanced ˈArbequinaˈ growth indices (main shoot length and growth quality). The results of genetic stability of callus induced shoots (CIS) and meristem induced shoots (MIS) revealed that 2C DNA value assessed by partec flow cytometery (FCM) had 0.01, 0.03 and 0.08 pg discrepencies in ˈAminˈ, ˈArbequinaˈ and ˈMeshkatˈ, repectively. The Amplified Fragment Length Polymorphism (AFLP) results also indicated that the cultivars were classified regardless of the micropropagation origin (CIS or MIS), except for ˈArbequinaˈ. The AFLP findings showed that ˈArbequinaˈ had the highest dispersal (7–38%) in CIS and MIS, while the Iranian cultivar of ˈMeshkatˈ (5–9%) had the highest stability. CONCLUSIONS: This study indicated the importance of in vitro growth parameters for improving the micropropagation indices of olive cultivars. It showed that optimized protocols (OM, PMB, zeatin, mannitol and 2000 cd sr m(−2)) co-produced larger calli resulting in indirect organogenesis. Based on FCM and AFLP analysis, it can be concluded that true-to-typeness of micropropagated olive was cultivar-dependent. BioMed Central 2021-03-29 /pmc/articles/PMC8008589/ /pubmed/33781291 http://dx.doi.org/10.1186/s13007-021-00724-7 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Mirzaei, Leila Yadollahi, Abbas Kermani, Maryam Jafarkhani Naderpour, Masoud Zeinanloo, Ali Asghar Farsi, Maryam Davoodi, Dariush Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
title | Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
title_full | Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
title_fullStr | Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
title_full_unstemmed | Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
title_short | Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
title_sort | evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008589/ https://www.ncbi.nlm.nih.gov/pubmed/33781291 http://dx.doi.org/10.1186/s13007-021-00724-7 |
work_keys_str_mv | AT mirzaeileila evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques AT yadollahiabbas evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques AT kermanimaryamjafarkhani evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques AT naderpourmasoud evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques AT zeinanlooaliasghar evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques AT farsimaryam evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques AT davoodidariush evaluationofgeneticstabilityinolivecallusinducedandmeristeminducedshootsusingflowcytometryandamplifiedfragmentlengthpolymorphismtechniques |