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Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries
Plant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different classes of polysaccharides, proteoglycans, and aromatic polymers and are one of the most complex...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008654/ https://www.ncbi.nlm.nih.gov/pubmed/33781321 http://dx.doi.org/10.1186/s13068-021-01922-0 |
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author | DeVree, Brian T Steiner, Lisa M Głazowska, Sylwia Ruhnow, Felix Herburger, Klaus Persson, Staffan Mravec, Jozef |
author_facet | DeVree, Brian T Steiner, Lisa M Głazowska, Sylwia Ruhnow, Felix Herburger, Klaus Persson, Staffan Mravec, Jozef |
author_sort | DeVree, Brian T |
collection | PubMed |
description | Plant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different classes of polysaccharides, proteoglycans, and aromatic polymers and are one of the most complex structures in Nature. One of the most challenging tasks of cell biology and biomass biotechnology research is to image the structure and organization of this complex matrix, as well as to visualize the compartmentalized, multiplayer biosynthetic machineries that build the elaborate cell wall architecture. Better knowledge of the plant cells, cell walls, and whole tissue is essential for bioengineering efforts and for designing efficient strategies of industrial deconstruction of the cell wall-derived biomass and its saccharification. Cell wall-directed molecular probes and analysis by light microscopy, which is capable of imaging with a high level of specificity, little sample processing, and often in real time, are important tools to understand cell wall assemblies. This review provides a comprehensive overview about the possibilities for fluorescence label-based imaging techniques and a variety of probing methods, discussing both well-established and emerging tools. Examples of applications of these tools are provided. We also list and discuss the advantages and limitations of the methods. Specifically, we elaborate on what are the most important considerations when applying a particular technique for plants, the potential for future development, and how the plant cell wall field might be inspired by advances in the biomedical and general cell biology fields. |
format | Online Article Text |
id | pubmed-8008654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80086542021-03-31 Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries DeVree, Brian T Steiner, Lisa M Głazowska, Sylwia Ruhnow, Felix Herburger, Klaus Persson, Staffan Mravec, Jozef Biotechnol Biofuels Review Plant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different classes of polysaccharides, proteoglycans, and aromatic polymers and are one of the most complex structures in Nature. One of the most challenging tasks of cell biology and biomass biotechnology research is to image the structure and organization of this complex matrix, as well as to visualize the compartmentalized, multiplayer biosynthetic machineries that build the elaborate cell wall architecture. Better knowledge of the plant cells, cell walls, and whole tissue is essential for bioengineering efforts and for designing efficient strategies of industrial deconstruction of the cell wall-derived biomass and its saccharification. Cell wall-directed molecular probes and analysis by light microscopy, which is capable of imaging with a high level of specificity, little sample processing, and often in real time, are important tools to understand cell wall assemblies. This review provides a comprehensive overview about the possibilities for fluorescence label-based imaging techniques and a variety of probing methods, discussing both well-established and emerging tools. Examples of applications of these tools are provided. We also list and discuss the advantages and limitations of the methods. Specifically, we elaborate on what are the most important considerations when applying a particular technique for plants, the potential for future development, and how the plant cell wall field might be inspired by advances in the biomedical and general cell biology fields. BioMed Central 2021-03-29 /pmc/articles/PMC8008654/ /pubmed/33781321 http://dx.doi.org/10.1186/s13068-021-01922-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Review DeVree, Brian T Steiner, Lisa M Głazowska, Sylwia Ruhnow, Felix Herburger, Klaus Persson, Staffan Mravec, Jozef Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
title | Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
title_full | Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
title_fullStr | Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
title_full_unstemmed | Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
title_short | Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
title_sort | current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008654/ https://www.ncbi.nlm.nih.gov/pubmed/33781321 http://dx.doi.org/10.1186/s13068-021-01922-0 |
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