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Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor
SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008786/ https://www.ncbi.nlm.nih.gov/pubmed/33823464 http://dx.doi.org/10.1016/j.bios.2021.113206 |
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author | Li, Jiahao Wu, Ding Yu, Yi Li, Tingxian Li, Kun Xiao, Meng-Meng Li, Yirong Zhang, Zhi-Yong Zhang, Guo-Jun |
author_facet | Li, Jiahao Wu, Ding Yu, Yi Li, Tingxian Li, Kun Xiao, Meng-Meng Li, Yirong Zhang, Zhi-Yong Zhang, Guo-Jun |
author_sort | Li, Jiahao |
collection | PubMed |
description | SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients’ samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis. |
format | Online Article Text |
id | pubmed-8008786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80087862021-03-31 Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor Li, Jiahao Wu, Ding Yu, Yi Li, Tingxian Li, Kun Xiao, Meng-Meng Li, Yirong Zhang, Zhi-Yong Zhang, Guo-Jun Biosens Bioelectron Article SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients’ samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis. Elsevier B.V. 2021-07-01 2021-03-30 /pmc/articles/PMC8008786/ /pubmed/33823464 http://dx.doi.org/10.1016/j.bios.2021.113206 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Li, Jiahao Wu, Ding Yu, Yi Li, Tingxian Li, Kun Xiao, Meng-Meng Li, Yirong Zhang, Zhi-Yong Zhang, Guo-Jun Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |
title | Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |
title_full | Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |
title_fullStr | Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |
title_full_unstemmed | Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |
title_short | Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |
title_sort | rapid and unamplified identification of covid-19 with morpholino-modified graphene field-effect transistor nanosensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008786/ https://www.ncbi.nlm.nih.gov/pubmed/33823464 http://dx.doi.org/10.1016/j.bios.2021.113206 |
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