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Mapping the micro-proteome of the nuclear lamina and lamina-associated domains

The nuclear lamina is a proteinaceous network of filaments that provide both structural and gene regulatory functions by tethering proteins and large domains of DNA, the so-called lamina-associated domains (LADs), to the periphery of the nucleus. LADs are a large fraction of the mammalian genome tha...

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Autores principales: Wong, Xianrong, Cutler, Jevon A, Hoskins, Victoria E, Gordon, Molly, Madugundu, Anil K, Pandey, Akhilesh, Reddy, Karen L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008952/
https://www.ncbi.nlm.nih.gov/pubmed/33758005
http://dx.doi.org/10.26508/lsa.202000774
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author Wong, Xianrong
Cutler, Jevon A
Hoskins, Victoria E
Gordon, Molly
Madugundu, Anil K
Pandey, Akhilesh
Reddy, Karen L
author_facet Wong, Xianrong
Cutler, Jevon A
Hoskins, Victoria E
Gordon, Molly
Madugundu, Anil K
Pandey, Akhilesh
Reddy, Karen L
author_sort Wong, Xianrong
collection PubMed
description The nuclear lamina is a proteinaceous network of filaments that provide both structural and gene regulatory functions by tethering proteins and large domains of DNA, the so-called lamina-associated domains (LADs), to the periphery of the nucleus. LADs are a large fraction of the mammalian genome that are repressed, in part, by their association to the nuclear periphery. The genesis and maintenance of LADs is poorly understood as are the proteins that participate in these functions. In an effort to identify proteins that reside at the nuclear periphery and potentially interact with LADs, we have taken a two-pronged approach. First, we have undertaken an interactome analysis of the inner nuclear membrane bound LAP2β to further characterize the nuclear lamina proteome. To accomplish this, we have leveraged the BioID system, which previously has been successfully used to characterize the nuclear lamina proteome. Second, we have established a system to identify proteins that bind to LADs by developing a chromatin-directed BioID system. We combined the BioID system with the m6A-tracer system which binds to LADs in live cells to identify both LAD proximal and nuclear lamina proteins. In combining these datasets, we have further characterized the protein network at the nuclear lamina, identified putative LAD proximal proteins and found several proteins that appear to interface with both micro-proteomes. Importantly, several proteins essential for LAD function, including heterochromatin regulating proteins related to H3K9 methylation, were identified in this study.
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spelling pubmed-80089522021-04-02 Mapping the micro-proteome of the nuclear lamina and lamina-associated domains Wong, Xianrong Cutler, Jevon A Hoskins, Victoria E Gordon, Molly Madugundu, Anil K Pandey, Akhilesh Reddy, Karen L Life Sci Alliance Research Articles The nuclear lamina is a proteinaceous network of filaments that provide both structural and gene regulatory functions by tethering proteins and large domains of DNA, the so-called lamina-associated domains (LADs), to the periphery of the nucleus. LADs are a large fraction of the mammalian genome that are repressed, in part, by their association to the nuclear periphery. The genesis and maintenance of LADs is poorly understood as are the proteins that participate in these functions. In an effort to identify proteins that reside at the nuclear periphery and potentially interact with LADs, we have taken a two-pronged approach. First, we have undertaken an interactome analysis of the inner nuclear membrane bound LAP2β to further characterize the nuclear lamina proteome. To accomplish this, we have leveraged the BioID system, which previously has been successfully used to characterize the nuclear lamina proteome. Second, we have established a system to identify proteins that bind to LADs by developing a chromatin-directed BioID system. We combined the BioID system with the m6A-tracer system which binds to LADs in live cells to identify both LAD proximal and nuclear lamina proteins. In combining these datasets, we have further characterized the protein network at the nuclear lamina, identified putative LAD proximal proteins and found several proteins that appear to interface with both micro-proteomes. Importantly, several proteins essential for LAD function, including heterochromatin regulating proteins related to H3K9 methylation, were identified in this study. Life Science Alliance LLC 2021-03-23 /pmc/articles/PMC8008952/ /pubmed/33758005 http://dx.doi.org/10.26508/lsa.202000774 Text en © 2021 Wong et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Wong, Xianrong
Cutler, Jevon A
Hoskins, Victoria E
Gordon, Molly
Madugundu, Anil K
Pandey, Akhilesh
Reddy, Karen L
Mapping the micro-proteome of the nuclear lamina and lamina-associated domains
title Mapping the micro-proteome of the nuclear lamina and lamina-associated domains
title_full Mapping the micro-proteome of the nuclear lamina and lamina-associated domains
title_fullStr Mapping the micro-proteome of the nuclear lamina and lamina-associated domains
title_full_unstemmed Mapping the micro-proteome of the nuclear lamina and lamina-associated domains
title_short Mapping the micro-proteome of the nuclear lamina and lamina-associated domains
title_sort mapping the micro-proteome of the nuclear lamina and lamina-associated domains
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008952/
https://www.ncbi.nlm.nih.gov/pubmed/33758005
http://dx.doi.org/10.26508/lsa.202000774
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