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Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis
BACKGROUND/AIMS: The present study aimed to investigate whether tocotrienol regulates interleukin 17 (IL-17)-induced osteoclastogenesis in rheumatoid arthritis (RA). METHODS: We evaluated the effect of tocotrienol on IL-17-induced receptor activator of nuclear factor kappa B ligand (RANKL) productio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Association of Internal Medicine
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009144/ https://www.ncbi.nlm.nih.gov/pubmed/32550719 http://dx.doi.org/10.3904/kjim.2019.372 |
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author | Kim, Kyoung-Woon Kim, Bo-Mi Won, Ji-Yeon Min, Hong Ki Lee, Seoung Joon Lee, Sang-Heon Kim, Hae-Rim |
author_facet | Kim, Kyoung-Woon Kim, Bo-Mi Won, Ji-Yeon Min, Hong Ki Lee, Seoung Joon Lee, Sang-Heon Kim, Hae-Rim |
author_sort | Kim, Kyoung-Woon |
collection | PubMed |
description | BACKGROUND/AIMS: The present study aimed to investigate whether tocotrienol regulates interleukin 17 (IL-17)-induced osteoclastogenesis in rheumatoid arthritis (RA). METHODS: We evaluated the effect of tocotrienol on IL-17-induced receptor activator of nuclear factor kappa B ligand (RANKL) production using RA fibroblast-like synoviocyte (FLS), together with real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Osteoclast differentiation was confirmed after culturing IL-17-treated RA FLS and Th17 cells with tocotrienol and monocytes. We analyzed the suppressive effect of tocotrienol on Th17 cells percentage or Th17-cytokine levels among peripheral blood mononuclear cells using flow cytometry. RESULTS: We found that IL-17 stimulated FLS to produce RANKL and tocotrienol decreased this IL-17-induced RANKL production. Tocotrienol decreased the IL-17-induced activation of mammalian target of rapamycin, extracellular signal-regulated kinase, and inhibitor of kappa B-alpha. When monocytes were incubated with IL-17, RANKL, IL-17-treated FLS or Th17 cells, osteoclasts were differentiated and tocotrienol decreased this osteoclast differentiation. Tocotrienol reduced Th17 cell differentiation and the production of IL-17 and sRANKL; however, tocotrienol did not affect Treg cell differentiation. CONCLUSIONS: Tocotrienol inhibited IL-17- activated RANKL production in RA FLS and IL-17-activated osteoclast formation. In addition, tocotrienol reduced Th17 differentiation. Therefore, tocotrienol could be a new therapeutic choice to treat bone destructive processes in RA. |
format | Online Article Text |
id | pubmed-8009144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-80091442021-04-02 Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis Kim, Kyoung-Woon Kim, Bo-Mi Won, Ji-Yeon Min, Hong Ki Lee, Seoung Joon Lee, Sang-Heon Kim, Hae-Rim Korean J Intern Med Original Article BACKGROUND/AIMS: The present study aimed to investigate whether tocotrienol regulates interleukin 17 (IL-17)-induced osteoclastogenesis in rheumatoid arthritis (RA). METHODS: We evaluated the effect of tocotrienol on IL-17-induced receptor activator of nuclear factor kappa B ligand (RANKL) production using RA fibroblast-like synoviocyte (FLS), together with real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Osteoclast differentiation was confirmed after culturing IL-17-treated RA FLS and Th17 cells with tocotrienol and monocytes. We analyzed the suppressive effect of tocotrienol on Th17 cells percentage or Th17-cytokine levels among peripheral blood mononuclear cells using flow cytometry. RESULTS: We found that IL-17 stimulated FLS to produce RANKL and tocotrienol decreased this IL-17-induced RANKL production. Tocotrienol decreased the IL-17-induced activation of mammalian target of rapamycin, extracellular signal-regulated kinase, and inhibitor of kappa B-alpha. When monocytes were incubated with IL-17, RANKL, IL-17-treated FLS or Th17 cells, osteoclasts were differentiated and tocotrienol decreased this osteoclast differentiation. Tocotrienol reduced Th17 cell differentiation and the production of IL-17 and sRANKL; however, tocotrienol did not affect Treg cell differentiation. CONCLUSIONS: Tocotrienol inhibited IL-17- activated RANKL production in RA FLS and IL-17-activated osteoclast formation. In addition, tocotrienol reduced Th17 differentiation. Therefore, tocotrienol could be a new therapeutic choice to treat bone destructive processes in RA. The Korean Association of Internal Medicine 2021-03 2020-06-19 /pmc/articles/PMC8009144/ /pubmed/32550719 http://dx.doi.org/10.3904/kjim.2019.372 Text en Copyright © 2021 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Kyoung-Woon Kim, Bo-Mi Won, Ji-Yeon Min, Hong Ki Lee, Seoung Joon Lee, Sang-Heon Kim, Hae-Rim Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
title | Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
title_full | Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
title_fullStr | Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
title_full_unstemmed | Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
title_short | Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
title_sort | tocotrienol regulates osteoclastogenesis in rheumatoid arthritis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009144/ https://www.ncbi.nlm.nih.gov/pubmed/32550719 http://dx.doi.org/10.3904/kjim.2019.372 |
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