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Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario

Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors an...

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Autores principales: D'Amico, Francesco, Bertacco, Alessandra, Finotti, Michele, Di Renzo, Chiara, Rodriguez-Davalos, Manuel I., Gondolesi, Gabriel E., Cillo, Umberto, Mulligan, David, Geibel, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009296/
https://www.ncbi.nlm.nih.gov/pubmed/33796547
http://dx.doi.org/10.3389/fsurg.2021.621525
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author D'Amico, Francesco
Bertacco, Alessandra
Finotti, Michele
Di Renzo, Chiara
Rodriguez-Davalos, Manuel I.
Gondolesi, Gabriel E.
Cillo, Umberto
Mulligan, David
Geibel, John
author_facet D'Amico, Francesco
Bertacco, Alessandra
Finotti, Michele
Di Renzo, Chiara
Rodriguez-Davalos, Manuel I.
Gondolesi, Gabriel E.
Cillo, Umberto
Mulligan, David
Geibel, John
author_sort D'Amico, Francesco
collection PubMed
description Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Results: We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). Conclusions: We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant.
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spelling pubmed-80092962021-03-31 Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario D'Amico, Francesco Bertacco, Alessandra Finotti, Michele Di Renzo, Chiara Rodriguez-Davalos, Manuel I. Gondolesi, Gabriel E. Cillo, Umberto Mulligan, David Geibel, John Front Surg Surgery Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Results: We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). Conclusions: We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant. Frontiers Media S.A. 2021-03-16 /pmc/articles/PMC8009296/ /pubmed/33796547 http://dx.doi.org/10.3389/fsurg.2021.621525 Text en Copyright © 2021 D'Amico, Bertacco, Finotti, Di Renzo, Rodriguez-Davalos, Gondolesi, Cillo, Mulligan and Geibel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Surgery
D'Amico, Francesco
Bertacco, Alessandra
Finotti, Michele
Di Renzo, Chiara
Rodriguez-Davalos, Manuel I.
Gondolesi, Gabriel E.
Cillo, Umberto
Mulligan, David
Geibel, John
Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
title Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
title_full Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
title_fullStr Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
title_full_unstemmed Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
title_short Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
title_sort bile microbiota in liver transplantation: proof of concept using gene amplification in a heterogeneous clinical scenario
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009296/
https://www.ncbi.nlm.nih.gov/pubmed/33796547
http://dx.doi.org/10.3389/fsurg.2021.621525
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