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Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors an...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009296/ https://www.ncbi.nlm.nih.gov/pubmed/33796547 http://dx.doi.org/10.3389/fsurg.2021.621525 |
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author | D'Amico, Francesco Bertacco, Alessandra Finotti, Michele Di Renzo, Chiara Rodriguez-Davalos, Manuel I. Gondolesi, Gabriel E. Cillo, Umberto Mulligan, David Geibel, John |
author_facet | D'Amico, Francesco Bertacco, Alessandra Finotti, Michele Di Renzo, Chiara Rodriguez-Davalos, Manuel I. Gondolesi, Gabriel E. Cillo, Umberto Mulligan, David Geibel, John |
author_sort | D'Amico, Francesco |
collection | PubMed |
description | Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Results: We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). Conclusions: We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant. |
format | Online Article Text |
id | pubmed-8009296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80092962021-03-31 Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario D'Amico, Francesco Bertacco, Alessandra Finotti, Michele Di Renzo, Chiara Rodriguez-Davalos, Manuel I. Gondolesi, Gabriel E. Cillo, Umberto Mulligan, David Geibel, John Front Surg Surgery Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Results: We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). Conclusions: We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant. Frontiers Media S.A. 2021-03-16 /pmc/articles/PMC8009296/ /pubmed/33796547 http://dx.doi.org/10.3389/fsurg.2021.621525 Text en Copyright © 2021 D'Amico, Bertacco, Finotti, Di Renzo, Rodriguez-Davalos, Gondolesi, Cillo, Mulligan and Geibel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Surgery D'Amico, Francesco Bertacco, Alessandra Finotti, Michele Di Renzo, Chiara Rodriguez-Davalos, Manuel I. Gondolesi, Gabriel E. Cillo, Umberto Mulligan, David Geibel, John Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario |
title | Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario |
title_full | Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario |
title_fullStr | Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario |
title_full_unstemmed | Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario |
title_short | Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario |
title_sort | bile microbiota in liver transplantation: proof of concept using gene amplification in a heterogeneous clinical scenario |
topic | Surgery |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009296/ https://www.ncbi.nlm.nih.gov/pubmed/33796547 http://dx.doi.org/10.3389/fsurg.2021.621525 |
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