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Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium
Although the thin endometrium (TE) has been widely recognized as a critical factor in implantation failure, the contribution of miRNA–mRNA regulatory network to the development of disease etiology remains to be further elucidated. This study performed an integrative analysis of the miRNA–mRNA expres...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009322/ https://www.ncbi.nlm.nih.gov/pubmed/33796129 http://dx.doi.org/10.3389/fgene.2021.589408 |
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author | Zong, Lu Zheng, Shengxia Meng, Ye Tang, Wenjuan Li, Daojing Wang, Zhenyun Tong, Xianhong Xu, Bo |
author_facet | Zong, Lu Zheng, Shengxia Meng, Ye Tang, Wenjuan Li, Daojing Wang, Zhenyun Tong, Xianhong Xu, Bo |
author_sort | Zong, Lu |
collection | PubMed |
description | Although the thin endometrium (TE) has been widely recognized as a critical factor in implantation failure, the contribution of miRNA–mRNA regulatory network to the development of disease etiology remains to be further elucidated. This study performed an integrative analysis of the miRNA–mRNA expression profiles in the thin and adjacent normal endometrium of eight patients with intrauterine adhesion to construct the transcriptomic regulatory networks. A total of 1,093 differentially expressed genes (DEGs) and 72 differentially expressed miRNAs (DEMs) were identified in the thin adhesive endometrium of the TE group compared with the control adjacent normal endometrial cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that the DEGs and the target genes of DEM were significantly enriched in angiogenesis, cell growth regulation, and Wnt signaling pathway. Multiple hub genes (CAV1, MET, MAL2, has-mir-138, ARHGAP6, CLIC4, RRAS, AGFG1, has-mir-200, and has-mir-429) were identified by constructing the miRNA–mRNA regulatory networks. Furthermore, a miRNA–mRNA pathway function analysis was conducted, and the hub genes were enriched in the FoxO signaling pathway, cell growth regulation, inflammatory response regulation, and regulation of autophagy pathways. Our study is the first to perform integrated mRNA-seq and miRNA-seq analyses in the thin adhesive endometrium and the control adjacent normal endometrial cells. This study provides new insights into the molecular mechanisms underlying the formation of thin endometrium. |
format | Online Article Text |
id | pubmed-8009322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80093222021-03-31 Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium Zong, Lu Zheng, Shengxia Meng, Ye Tang, Wenjuan Li, Daojing Wang, Zhenyun Tong, Xianhong Xu, Bo Front Genet Genetics Although the thin endometrium (TE) has been widely recognized as a critical factor in implantation failure, the contribution of miRNA–mRNA regulatory network to the development of disease etiology remains to be further elucidated. This study performed an integrative analysis of the miRNA–mRNA expression profiles in the thin and adjacent normal endometrium of eight patients with intrauterine adhesion to construct the transcriptomic regulatory networks. A total of 1,093 differentially expressed genes (DEGs) and 72 differentially expressed miRNAs (DEMs) were identified in the thin adhesive endometrium of the TE group compared with the control adjacent normal endometrial cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that the DEGs and the target genes of DEM were significantly enriched in angiogenesis, cell growth regulation, and Wnt signaling pathway. Multiple hub genes (CAV1, MET, MAL2, has-mir-138, ARHGAP6, CLIC4, RRAS, AGFG1, has-mir-200, and has-mir-429) were identified by constructing the miRNA–mRNA regulatory networks. Furthermore, a miRNA–mRNA pathway function analysis was conducted, and the hub genes were enriched in the FoxO signaling pathway, cell growth regulation, inflammatory response regulation, and regulation of autophagy pathways. Our study is the first to perform integrated mRNA-seq and miRNA-seq analyses in the thin adhesive endometrium and the control adjacent normal endometrial cells. This study provides new insights into the molecular mechanisms underlying the formation of thin endometrium. Frontiers Media S.A. 2021-03-16 /pmc/articles/PMC8009322/ /pubmed/33796129 http://dx.doi.org/10.3389/fgene.2021.589408 Text en Copyright © 2021 Zong, Zheng, Meng, Tang, Li, Wang, Tong and Xu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zong, Lu Zheng, Shengxia Meng, Ye Tang, Wenjuan Li, Daojing Wang, Zhenyun Tong, Xianhong Xu, Bo Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium |
title | Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium |
title_full | Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium |
title_fullStr | Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium |
title_full_unstemmed | Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium |
title_short | Integrated Transcriptomic Analysis of the miRNA–mRNA Interaction Network in Thin Endometrium |
title_sort | integrated transcriptomic analysis of the mirna–mrna interaction network in thin endometrium |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009322/ https://www.ncbi.nlm.nih.gov/pubmed/33796129 http://dx.doi.org/10.3389/fgene.2021.589408 |
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