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High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission

Bovine tuberculosis (bTB) caused by Mycobacterium bovis (M. bovis) represents one of major zoonotic diseases among cattle, it also affects the health of human, other domestic animals and wild life populations. Inhalation of infected aerosol droplets is considered as the most frequent route of the in...

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Autores principales: Xu, Fang, Tian, Lili, Li, Yan, Zhang, Xuelian, Qi, Yayin, Jing, Zhigang, Pan, Yangyang, Zhang, Li, Fan, Xiaoxu, Wang, Meng, Zeng, Qiaoying, Fan, Weixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009431/
https://www.ncbi.nlm.nih.gov/pubmed/33784364
http://dx.doi.org/10.1371/journal.pone.0249341
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author Xu, Fang
Tian, Lili
Li, Yan
Zhang, Xuelian
Qi, Yayin
Jing, Zhigang
Pan, Yangyang
Zhang, Li
Fan, Xiaoxu
Wang, Meng
Zeng, Qiaoying
Fan, Weixing
author_facet Xu, Fang
Tian, Lili
Li, Yan
Zhang, Xuelian
Qi, Yayin
Jing, Zhigang
Pan, Yangyang
Zhang, Li
Fan, Xiaoxu
Wang, Meng
Zeng, Qiaoying
Fan, Weixing
author_sort Xu, Fang
collection PubMed
description Bovine tuberculosis (bTB) caused by Mycobacterium bovis (M. bovis) represents one of major zoonotic diseases among cattle, it also affects the health of human, other domestic animals and wild life populations. Inhalation of infected aerosol droplets is considered as the most frequent route of the infection. This study aims to investigate the current forms of tuberculosis in cattle and identify the possible transmission modes in dairy farms of China. 13,345 cows from eight dairy farms in three provinces were comprehensively diagnosed by a multitude of assays, including SIT, CIT, IFN-γ assay and ELISA. It has been indicated that advanced infection of bTB was found in 752 (5.64%) cattle, suggesting a high prevalence of tuberculosis in these dairy farms. In the necropsy examination of 151 positive cattle, typical bTB lesions were observed in 131 cattle (86.75%), of which, notably, 90.84% lesions appeared in liver, spleen, mesenteric lymph nodes, mammary lymph nodes and other organs, taking up a large proportion among cattle with advanced bTB infection. 71.26% extrapulmonary tuberculosis (EPTB) was related to gastrointestinal system. M. bovis nucleic acid was further found in milk and feces samples and M. bovis was even isolated from milk samples. Phylogenetic analysis based on whole genome sequencing unraveled that six isolates were closely related to M. bovis AF2122/97 originated from UK, whereas four isolates shared close relation to M. bovis 30 from China, respectively. Our data demonstrate that the increase of EPTB transmitted by digestive tract is implicated in the current high prevalence rate of bTB in China, which also provides leads for bTB control in other countries with high prevalence of bTB in the future.
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spelling pubmed-80094312021-04-07 High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission Xu, Fang Tian, Lili Li, Yan Zhang, Xuelian Qi, Yayin Jing, Zhigang Pan, Yangyang Zhang, Li Fan, Xiaoxu Wang, Meng Zeng, Qiaoying Fan, Weixing PLoS One Research Article Bovine tuberculosis (bTB) caused by Mycobacterium bovis (M. bovis) represents one of major zoonotic diseases among cattle, it also affects the health of human, other domestic animals and wild life populations. Inhalation of infected aerosol droplets is considered as the most frequent route of the infection. This study aims to investigate the current forms of tuberculosis in cattle and identify the possible transmission modes in dairy farms of China. 13,345 cows from eight dairy farms in three provinces were comprehensively diagnosed by a multitude of assays, including SIT, CIT, IFN-γ assay and ELISA. It has been indicated that advanced infection of bTB was found in 752 (5.64%) cattle, suggesting a high prevalence of tuberculosis in these dairy farms. In the necropsy examination of 151 positive cattle, typical bTB lesions were observed in 131 cattle (86.75%), of which, notably, 90.84% lesions appeared in liver, spleen, mesenteric lymph nodes, mammary lymph nodes and other organs, taking up a large proportion among cattle with advanced bTB infection. 71.26% extrapulmonary tuberculosis (EPTB) was related to gastrointestinal system. M. bovis nucleic acid was further found in milk and feces samples and M. bovis was even isolated from milk samples. Phylogenetic analysis based on whole genome sequencing unraveled that six isolates were closely related to M. bovis AF2122/97 originated from UK, whereas four isolates shared close relation to M. bovis 30 from China, respectively. Our data demonstrate that the increase of EPTB transmitted by digestive tract is implicated in the current high prevalence rate of bTB in China, which also provides leads for bTB control in other countries with high prevalence of bTB in the future. Public Library of Science 2021-03-30 /pmc/articles/PMC8009431/ /pubmed/33784364 http://dx.doi.org/10.1371/journal.pone.0249341 Text en © 2021 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xu, Fang
Tian, Lili
Li, Yan
Zhang, Xuelian
Qi, Yayin
Jing, Zhigang
Pan, Yangyang
Zhang, Li
Fan, Xiaoxu
Wang, Meng
Zeng, Qiaoying
Fan, Weixing
High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission
title High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission
title_full High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission
title_fullStr High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission
title_full_unstemmed High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission
title_short High prevalence of extrapulmonary tuberculosis in dairy farms: Evidence for possible gastrointestinal transmission
title_sort high prevalence of extrapulmonary tuberculosis in dairy farms: evidence for possible gastrointestinal transmission
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009431/
https://www.ncbi.nlm.nih.gov/pubmed/33784364
http://dx.doi.org/10.1371/journal.pone.0249341
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