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Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway

INTRODUCTION: Inflammation is one of the main causes of impaired health in livestock and some of its processes weaken animal productivity and impact human health. The present study was conducted to evaluate the effect of echinacea extract (cichoric acid – CA) on yak peripheral blood mononuclear cell...

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Autores principales: Xue, Cai-hua, A, Shun-xian, Wang, Meng-jie, Wu, Qiang, Liu, Jia-hua, Zhang, Long-fei, Wu, Yun, Wu, Hua, Chai, Sha-tuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009576/
https://www.ncbi.nlm.nih.gov/pubmed/33817403
http://dx.doi.org/10.2478/jvetres-2021-0016
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author Xue, Cai-hua
A, Shun-xian
Wang, Meng-jie
Wu, Qiang
Liu, Jia-hua
Zhang, Long-fei
Wu, Yun
Wu, Hua
Chai, Sha-tuo
author_facet Xue, Cai-hua
A, Shun-xian
Wang, Meng-jie
Wu, Qiang
Liu, Jia-hua
Zhang, Long-fei
Wu, Yun
Wu, Hua
Chai, Sha-tuo
author_sort Xue, Cai-hua
collection PubMed
description INTRODUCTION: Inflammation is one of the main causes of impaired health in livestock and some of its processes weaken animal productivity and impact human health. The present study was conducted to evaluate the effect of echinacea extract (cichoric acid – CA) on yak peripheral blood mononuclear cells (PBMCs), inflammatory-related factors, and the toll-like receptor (TLR)4 signalling pathway induced by lipopolysaccharide (LPS) in these PBMCs. MATERIAL AND METHODS: Yak PBMCs were co-cultured with LPS and CA in vitro. The proliferative activity of cells was detected using the cell-counting kit-8 method, the optimal stimulation concentration of LPS was selected, the effect of CA on the content of inflammation-related factors was evaluated using an ELISA kit, and the mRNA expression of these factors was detected by RT-PCR. RESULTS: CA inhibited the inflammatory response of yak PBMCs induced by LPS. CA inhibited gene and protein expression of key nodes of the TLR4 signalling pathway in yak PBMCs. CONCLUSION: It is suggested that CA has anti-inflammatory and immunomodulatory effects on yak PBMCs via the TLR4 pathway.
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spelling pubmed-80095762021-04-02 Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway Xue, Cai-hua A, Shun-xian Wang, Meng-jie Wu, Qiang Liu, Jia-hua Zhang, Long-fei Wu, Yun Wu, Hua Chai, Sha-tuo J Vet Res Research Article INTRODUCTION: Inflammation is one of the main causes of impaired health in livestock and some of its processes weaken animal productivity and impact human health. The present study was conducted to evaluate the effect of echinacea extract (cichoric acid – CA) on yak peripheral blood mononuclear cells (PBMCs), inflammatory-related factors, and the toll-like receptor (TLR)4 signalling pathway induced by lipopolysaccharide (LPS) in these PBMCs. MATERIAL AND METHODS: Yak PBMCs were co-cultured with LPS and CA in vitro. The proliferative activity of cells was detected using the cell-counting kit-8 method, the optimal stimulation concentration of LPS was selected, the effect of CA on the content of inflammation-related factors was evaluated using an ELISA kit, and the mRNA expression of these factors was detected by RT-PCR. RESULTS: CA inhibited the inflammatory response of yak PBMCs induced by LPS. CA inhibited gene and protein expression of key nodes of the TLR4 signalling pathway in yak PBMCs. CONCLUSION: It is suggested that CA has anti-inflammatory and immunomodulatory effects on yak PBMCs via the TLR4 pathway. Sciendo 2021-03-09 /pmc/articles/PMC8009576/ /pubmed/33817403 http://dx.doi.org/10.2478/jvetres-2021-0016 Text en © 2021 C.H. Xue et al., published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
spellingShingle Research Article
Xue, Cai-hua
A, Shun-xian
Wang, Meng-jie
Wu, Qiang
Liu, Jia-hua
Zhang, Long-fei
Wu, Yun
Wu, Hua
Chai, Sha-tuo
Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway
title Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway
title_full Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway
title_fullStr Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway
title_full_unstemmed Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway
title_short Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway
title_sort echinacea purpurea extract (cichoric acid) exerts an anti-inflammatory effect on yak pbmcs and regulates the tlr4 signalling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009576/
https://www.ncbi.nlm.nih.gov/pubmed/33817403
http://dx.doi.org/10.2478/jvetres-2021-0016
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