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Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants
Phytochromes are photoreceptors regulating growth and development in plants. Using the model plant Arabidopsis, we identified a novel signalling pathway downstream of the far-red light-sensing phytochrome, phyA, that depends on the highly conserved CCR4-NOT complex. CCR4-NOT is integral to RNA metab...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009681/ https://www.ncbi.nlm.nih.gov/pubmed/33783355 http://dx.doi.org/10.7554/eLife.63697 |
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author | Schwenk, Philipp Sheerin, David J Ponnu, Jathish Staudt, Anne-Marie Lesch, Klara L Lichtenberg, Elisabeth Medzihradszky, Katalin F Hoecker, Ute Klement, Eva Viczián, András Hiltbrunner, Andreas |
author_facet | Schwenk, Philipp Sheerin, David J Ponnu, Jathish Staudt, Anne-Marie Lesch, Klara L Lichtenberg, Elisabeth Medzihradszky, Katalin F Hoecker, Ute Klement, Eva Viczián, András Hiltbrunner, Andreas |
author_sort | Schwenk, Philipp |
collection | PubMed |
description | Phytochromes are photoreceptors regulating growth and development in plants. Using the model plant Arabidopsis, we identified a novel signalling pathway downstream of the far-red light-sensing phytochrome, phyA, that depends on the highly conserved CCR4-NOT complex. CCR4-NOT is integral to RNA metabolism in yeast and animals, but its function in plants is largely unknown. NOT9B, an Arabidopsis homologue of human CNOT9, is a component of the CCR4-NOT complex, and acts as negative regulator of phyA-specific light signalling when bound to NOT1, the scaffold protein of the complex. Light-activated phyA interacts with and displaces NOT9B from NOT1, suggesting a potential mechanism for light signalling through CCR4-NOT. ARGONAUTE 1 and proteins involved in splicing associate with NOT9B and we show that NOT9B is required for specific phyA-dependent alternative splicing events. Furthermore, association with nuclear localised ARGONAUTE 1 raises the possibility that NOT9B and CCR4-NOT are involved in phyA-modulated gene expression. |
format | Online Article Text |
id | pubmed-8009681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80096812021-03-31 Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants Schwenk, Philipp Sheerin, David J Ponnu, Jathish Staudt, Anne-Marie Lesch, Klara L Lichtenberg, Elisabeth Medzihradszky, Katalin F Hoecker, Ute Klement, Eva Viczián, András Hiltbrunner, Andreas eLife Plant Biology Phytochromes are photoreceptors regulating growth and development in plants. Using the model plant Arabidopsis, we identified a novel signalling pathway downstream of the far-red light-sensing phytochrome, phyA, that depends on the highly conserved CCR4-NOT complex. CCR4-NOT is integral to RNA metabolism in yeast and animals, but its function in plants is largely unknown. NOT9B, an Arabidopsis homologue of human CNOT9, is a component of the CCR4-NOT complex, and acts as negative regulator of phyA-specific light signalling when bound to NOT1, the scaffold protein of the complex. Light-activated phyA interacts with and displaces NOT9B from NOT1, suggesting a potential mechanism for light signalling through CCR4-NOT. ARGONAUTE 1 and proteins involved in splicing associate with NOT9B and we show that NOT9B is required for specific phyA-dependent alternative splicing events. Furthermore, association with nuclear localised ARGONAUTE 1 raises the possibility that NOT9B and CCR4-NOT are involved in phyA-modulated gene expression. eLife Sciences Publications, Ltd 2021-03-30 /pmc/articles/PMC8009681/ /pubmed/33783355 http://dx.doi.org/10.7554/eLife.63697 Text en © 2021, Schwenk et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Plant Biology Schwenk, Philipp Sheerin, David J Ponnu, Jathish Staudt, Anne-Marie Lesch, Klara L Lichtenberg, Elisabeth Medzihradszky, Katalin F Hoecker, Ute Klement, Eva Viczián, András Hiltbrunner, Andreas Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants |
title | Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants |
title_full | Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants |
title_fullStr | Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants |
title_full_unstemmed | Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants |
title_short | Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants |
title_sort | uncovering a novel function of the ccr4-not complex in phytochrome a-mediated light signalling in plants |
topic | Plant Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009681/ https://www.ncbi.nlm.nih.gov/pubmed/33783355 http://dx.doi.org/10.7554/eLife.63697 |
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