A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers

In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of Burkholderia pseudomallei, a Gram-negative bacterium that is the...

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Autores principales: Gallian, Brandon, Zaeimian, Masoumeh Saber, Hau, Derrick, AuCoin, David, Zhu, Xiaoshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009851/
https://www.ncbi.nlm.nih.gov/pubmed/33816457
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author Gallian, Brandon
Zaeimian, Masoumeh Saber
Hau, Derrick
AuCoin, David
Zhu, Xiaoshan
author_facet Gallian, Brandon
Zaeimian, Masoumeh Saber
Hau, Derrick
AuCoin, David
Zhu, Xiaoshan
author_sort Gallian, Brandon
collection PubMed
description In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of Burkholderia pseudomallei, a Gram-negative bacterium that is the causative agent of melioidosis. CPS is a high molecular weight antigen displayed and is shed from the outer membrane of B. pseudomallei. The immunoassay using the time-gated platform presents a limit of detection at around 23 pg/ml when CPS is spiked in human serum.
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spelling pubmed-80098512021-07-01 A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers Gallian, Brandon Zaeimian, Masoumeh Saber Hau, Derrick AuCoin, David Zhu, Xiaoshan Front Phys Article In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of Burkholderia pseudomallei, a Gram-negative bacterium that is the causative agent of melioidosis. CPS is a high molecular weight antigen displayed and is shed from the outer membrane of B. pseudomallei. The immunoassay using the time-gated platform presents a limit of detection at around 23 pg/ml when CPS is spiked in human serum. 2021-01-27 2021-01 /pmc/articles/PMC8009851/ /pubmed/33816457 Text en This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Article
Gallian, Brandon
Zaeimian, Masoumeh Saber
Hau, Derrick
AuCoin, David
Zhu, Xiaoshan
A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers
title A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers
title_full A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers
title_fullStr A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers
title_full_unstemmed A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers
title_short A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers
title_sort highly sensitive time-gated fluorescence immunoassay platform using mn-doped agznins/zns nanocrystals as signal transducers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009851/
https://www.ncbi.nlm.nih.gov/pubmed/33816457
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