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Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession

The successional ecology of nitrogen cycling in biocrusts and the linkages to ecosystem processes remains unclear. To explore this, four successional stages of natural biocrust with five batches of repeated sampling and three developmental stages of simulated biocrust were studied using relative and...

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Autores principales: Wang, Qiong, Han, Yingchun, Lan, Shubin, Hu, Chunxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009985/
https://www.ncbi.nlm.nih.gov/pubmed/33815315
http://dx.doi.org/10.3389/fmicb.2021.633428
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author Wang, Qiong
Han, Yingchun
Lan, Shubin
Hu, Chunxiang
author_facet Wang, Qiong
Han, Yingchun
Lan, Shubin
Hu, Chunxiang
author_sort Wang, Qiong
collection PubMed
description The successional ecology of nitrogen cycling in biocrusts and the linkages to ecosystem processes remains unclear. To explore this, four successional stages of natural biocrust with five batches of repeated sampling and three developmental stages of simulated biocrust were studied using relative and absolute quantified multi-omics methods. A consistent pattern across all biocrust was found where ammonium assimilation, mineralization, dissimilatory nitrite to ammonium (DNiRA), and assimilatory nitrate to ammonium were abundant, while denitrification medium, N-fixation, and ammonia oxidation were low. Mathematic analysis showed that the nitrogen cycle in natural biocrust was driven by dissolved organic N and NO(3)(–). pH and SO(4)(2–) were the strongest variables affecting denitrification, while C:(N:P) was the strongest variable affecting N-fixation, DNiRA, nitrite oxidation, and dissimilatory nitrate to nitrite. Furthermore, N-fixation and DNiRA were closely related to elemental stoichiometry and redox balance, while assimilatory nitrite to ammonium (ANiRA) and mineralization were related to hydrological cycles. Together with the absolute quantification and network models, our results suggest that responsive ANiRA and mineralization decreased during biocrust succession; whereas central respiratory DNiRA, the final step of denitrification, and the complexity and interaction of the whole nitrogen cycle network increased. Therefore, our study stresses the changing environmental functions in the biocrust N-cycle, which are succession-dependent.
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spelling pubmed-80099852021-04-01 Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession Wang, Qiong Han, Yingchun Lan, Shubin Hu, Chunxiang Front Microbiol Microbiology The successional ecology of nitrogen cycling in biocrusts and the linkages to ecosystem processes remains unclear. To explore this, four successional stages of natural biocrust with five batches of repeated sampling and three developmental stages of simulated biocrust were studied using relative and absolute quantified multi-omics methods. A consistent pattern across all biocrust was found where ammonium assimilation, mineralization, dissimilatory nitrite to ammonium (DNiRA), and assimilatory nitrate to ammonium were abundant, while denitrification medium, N-fixation, and ammonia oxidation were low. Mathematic analysis showed that the nitrogen cycle in natural biocrust was driven by dissolved organic N and NO(3)(–). pH and SO(4)(2–) were the strongest variables affecting denitrification, while C:(N:P) was the strongest variable affecting N-fixation, DNiRA, nitrite oxidation, and dissimilatory nitrate to nitrite. Furthermore, N-fixation and DNiRA were closely related to elemental stoichiometry and redox balance, while assimilatory nitrite to ammonium (ANiRA) and mineralization were related to hydrological cycles. Together with the absolute quantification and network models, our results suggest that responsive ANiRA and mineralization decreased during biocrust succession; whereas central respiratory DNiRA, the final step of denitrification, and the complexity and interaction of the whole nitrogen cycle network increased. Therefore, our study stresses the changing environmental functions in the biocrust N-cycle, which are succession-dependent. Frontiers Media S.A. 2021-03-17 /pmc/articles/PMC8009985/ /pubmed/33815315 http://dx.doi.org/10.3389/fmicb.2021.633428 Text en Copyright © 2021 Wang, Han, Lan and Hu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Qiong
Han, Yingchun
Lan, Shubin
Hu, Chunxiang
Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession
title Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession
title_full Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession
title_fullStr Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession
title_full_unstemmed Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession
title_short Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession
title_sort metagenomic insight into patterns and mechanism of nitrogen cycle during biocrust succession
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009985/
https://www.ncbi.nlm.nih.gov/pubmed/33815315
http://dx.doi.org/10.3389/fmicb.2021.633428
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