Cargando…

Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel

Synergistic impairment of the blood-brain barrier (BBB) induced by methamphetamine (METH) and HIV-Tat protein increases the risk of HIV-associated neurocognitive disorders (HAND) in HIV-positive METH abusers. Studies have shown that oxidative stress plays a vital role in METH- and HIV-Tat-induced da...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Jian, Zhang, Ruilin, Wang, Shangwen, Zhang, Dongxian, Leung, Chi-Kwan, Yang, Genmeng, Li, Yuanyuan, Liu, Liu, Xu, Yue, Lin, Shucheng, Wang, Chan, Zeng, Xiaofeng, Li, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010131/
https://www.ncbi.nlm.nih.gov/pubmed/33815104
http://dx.doi.org/10.3389/fphar.2021.619436
_version_ 1783672998571540480
author Huang, Jian
Zhang, Ruilin
Wang, Shangwen
Zhang, Dongxian
Leung, Chi-Kwan
Yang, Genmeng
Li, Yuanyuan
Liu, Liu
Xu, Yue
Lin, Shucheng
Wang, Chan
Zeng, Xiaofeng
Li, Juan
author_facet Huang, Jian
Zhang, Ruilin
Wang, Shangwen
Zhang, Dongxian
Leung, Chi-Kwan
Yang, Genmeng
Li, Yuanyuan
Liu, Liu
Xu, Yue
Lin, Shucheng
Wang, Chan
Zeng, Xiaofeng
Li, Juan
author_sort Huang, Jian
collection PubMed
description Synergistic impairment of the blood-brain barrier (BBB) induced by methamphetamine (METH) and HIV-Tat protein increases the risk of HIV-associated neurocognitive disorders (HAND) in HIV-positive METH abusers. Studies have shown that oxidative stress plays a vital role in METH- and HIV-Tat-induced damage to the BBB but have not clarified the mechanism. This study uses the human brain microvascular endothelial cell line hCMEC/D3 and tree shrews to investigate whether the transient receptor potential melastatin 2 (TRPM2) channel, a cellular effector of the oxidative stress, might regulate synergistic damage to the BBB caused by METH and HIV-Tat. We showed that METH and HIV-Tat damaged the BBB in vitro, producing abnormal cell morphology, increased apoptosis, reduced protein expression of the tight junctions (TJ) including Junctional adhesion molecule A (JAMA) and Occludin, and a junctional associated protein Zonula occludens 1 (ZO1), and increased the flux of sodium fluorescein (NaF) across the hCMEC/D3 cells monolayer. METH and HIV-Tat co-induced the oxidative stress response, reducing catalase (CAT), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD) activity, as well as increased reactive oxygen species (ROS) and malonaldehyde (MDA) level. Pretreatment with n-acetylcysteine amide (NACA) alleviated the oxidative stress response and BBB damage characterized by improving cell morphology, viability, apoptosis levels, TJ protein expression levels, and NaF flux. METH and HIV-Tat co-induced the activation and high protein expression of the TRPM2 channel, however, early intervention using 8-Bromoadenosine-5′-O-diphosphoribose (8-Br-ADPR), an inhibitor of TPRM2 channel, or TRPM2 gene knockdown attenuated the BBB damage. Oxidative stress inhibition reduced the activation and high protein expression of the TRPM2 channel in the in vitro model, which in turn reduced the oxidative stress response. Further, 8-Br-ADPR attenuated the effects of METH and HIV-Tat on the BBB in tree shrews—namely, down-regulated TJ protein expression and increased BBB permeability to Evans blue (EB) and NaF. In summary, the TRPM2 channel can regulate METH- and HIV-Tat-induced oxidative stress and BBB injury, giving the channel potential for developing drug interventions to reduce BBB injury and neuropsychiatric symptoms in HIV-infected METH abusers.
format Online
Article
Text
id pubmed-8010131
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80101312021-04-01 Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel Huang, Jian Zhang, Ruilin Wang, Shangwen Zhang, Dongxian Leung, Chi-Kwan Yang, Genmeng Li, Yuanyuan Liu, Liu Xu, Yue Lin, Shucheng Wang, Chan Zeng, Xiaofeng Li, Juan Front Pharmacol Pharmacology Synergistic impairment of the blood-brain barrier (BBB) induced by methamphetamine (METH) and HIV-Tat protein increases the risk of HIV-associated neurocognitive disorders (HAND) in HIV-positive METH abusers. Studies have shown that oxidative stress plays a vital role in METH- and HIV-Tat-induced damage to the BBB but have not clarified the mechanism. This study uses the human brain microvascular endothelial cell line hCMEC/D3 and tree shrews to investigate whether the transient receptor potential melastatin 2 (TRPM2) channel, a cellular effector of the oxidative stress, might regulate synergistic damage to the BBB caused by METH and HIV-Tat. We showed that METH and HIV-Tat damaged the BBB in vitro, producing abnormal cell morphology, increased apoptosis, reduced protein expression of the tight junctions (TJ) including Junctional adhesion molecule A (JAMA) and Occludin, and a junctional associated protein Zonula occludens 1 (ZO1), and increased the flux of sodium fluorescein (NaF) across the hCMEC/D3 cells monolayer. METH and HIV-Tat co-induced the oxidative stress response, reducing catalase (CAT), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD) activity, as well as increased reactive oxygen species (ROS) and malonaldehyde (MDA) level. Pretreatment with n-acetylcysteine amide (NACA) alleviated the oxidative stress response and BBB damage characterized by improving cell morphology, viability, apoptosis levels, TJ protein expression levels, and NaF flux. METH and HIV-Tat co-induced the activation and high protein expression of the TRPM2 channel, however, early intervention using 8-Bromoadenosine-5′-O-diphosphoribose (8-Br-ADPR), an inhibitor of TPRM2 channel, or TRPM2 gene knockdown attenuated the BBB damage. Oxidative stress inhibition reduced the activation and high protein expression of the TRPM2 channel in the in vitro model, which in turn reduced the oxidative stress response. Further, 8-Br-ADPR attenuated the effects of METH and HIV-Tat on the BBB in tree shrews—namely, down-regulated TJ protein expression and increased BBB permeability to Evans blue (EB) and NaF. In summary, the TRPM2 channel can regulate METH- and HIV-Tat-induced oxidative stress and BBB injury, giving the channel potential for developing drug interventions to reduce BBB injury and neuropsychiatric symptoms in HIV-infected METH abusers. Frontiers Media S.A. 2021-03-17 /pmc/articles/PMC8010131/ /pubmed/33815104 http://dx.doi.org/10.3389/fphar.2021.619436 Text en Copyright © 2021 Huang, Zhang, Wang, Zhang, Leung, Yang, Li, Liu, Xu, Lin, Wang, Zeng and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Huang, Jian
Zhang, Ruilin
Wang, Shangwen
Zhang, Dongxian
Leung, Chi-Kwan
Yang, Genmeng
Li, Yuanyuan
Liu, Liu
Xu, Yue
Lin, Shucheng
Wang, Chan
Zeng, Xiaofeng
Li, Juan
Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel
title Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel
title_full Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel
title_fullStr Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel
title_full_unstemmed Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel
title_short Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel
title_sort methamphetamine and hiv-tat protein synergistically induce oxidative stress and blood-brain barrier damage via transient receptor potential melastatin 2 channel
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010131/
https://www.ncbi.nlm.nih.gov/pubmed/33815104
http://dx.doi.org/10.3389/fphar.2021.619436
work_keys_str_mv AT huangjian methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT zhangruilin methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT wangshangwen methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT zhangdongxian methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT leungchikwan methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT yanggenmeng methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT liyuanyuan methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT liuliu methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT xuyue methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT linshucheng methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT wangchan methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT zengxiaofeng methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel
AT lijuan methamphetamineandhivtatproteinsynergisticallyinduceoxidativestressandbloodbrainbarrierdamageviatransientreceptorpotentialmelastatin2channel