Cargando…

High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity

Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight los...

Descripción completa

Detalles Bibliográficos
Autores principales: Ho, Jan Hoong, Ong, Kwok Leung, Cuesta Torres, Luisa F., Liu, Yifen, Adam, Safwaan, Iqbal, Zohaib, Dhage, Shaishav, Ammori, Basil J., Syed, Akheel A., Rye, Kerry-Anne, Tabet, Fatiha, Soran, Handrean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010476/
https://www.ncbi.nlm.nih.gov/pubmed/33093236
http://dx.doi.org/10.1194/jlr.RA120000963
_version_ 1783673072831692800
author Ho, Jan Hoong
Ong, Kwok Leung
Cuesta Torres, Luisa F.
Liu, Yifen
Adam, Safwaan
Iqbal, Zohaib
Dhage, Shaishav
Ammori, Basil J.
Syed, Akheel A.
Rye, Kerry-Anne
Tabet, Fatiha
Soran, Handrean
author_facet Ho, Jan Hoong
Ong, Kwok Leung
Cuesta Torres, Luisa F.
Liu, Yifen
Adam, Safwaan
Iqbal, Zohaib
Dhage, Shaishav
Ammori, Basil J.
Syed, Akheel A.
Rye, Kerry-Anne
Tabet, Fatiha
Soran, Handrean
author_sort Ho, Jan Hoong
collection PubMed
description Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDLs were isolated from patients with severe obesity (n = 53) before and 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDLs were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222, and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222, and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222, and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase activity were increased and intercellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with an increase in cholesterol efflux capacity (r = 0.326, P = 0.027 and r = 0.349, P = 0.017, respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy participants versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction and may lead to the development of novel treatment strategies for obesity and related metabolic disorders.
format Online
Article
Text
id pubmed-8010476
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-80104762021-04-02 High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity Ho, Jan Hoong Ong, Kwok Leung Cuesta Torres, Luisa F. Liu, Yifen Adam, Safwaan Iqbal, Zohaib Dhage, Shaishav Ammori, Basil J. Syed, Akheel A. Rye, Kerry-Anne Tabet, Fatiha Soran, Handrean J Lipid Res Research Article Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDLs were isolated from patients with severe obesity (n = 53) before and 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDLs were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222, and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222, and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222, and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase activity were increased and intercellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with an increase in cholesterol efflux capacity (r = 0.326, P = 0.027 and r = 0.349, P = 0.017, respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy participants versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction and may lead to the development of novel treatment strategies for obesity and related metabolic disorders. American Society for Biochemistry and Molecular Biology 2021-02-06 /pmc/articles/PMC8010476/ /pubmed/33093236 http://dx.doi.org/10.1194/jlr.RA120000963 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Ho, Jan Hoong
Ong, Kwok Leung
Cuesta Torres, Luisa F.
Liu, Yifen
Adam, Safwaan
Iqbal, Zohaib
Dhage, Shaishav
Ammori, Basil J.
Syed, Akheel A.
Rye, Kerry-Anne
Tabet, Fatiha
Soran, Handrean
High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
title High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
title_full High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
title_fullStr High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
title_full_unstemmed High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
title_short High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
title_sort high density lipoprotein-associated mirna is increased following roux-en-y gastric bypass surgery for severe obesity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010476/
https://www.ncbi.nlm.nih.gov/pubmed/33093236
http://dx.doi.org/10.1194/jlr.RA120000963
work_keys_str_mv AT hojanhoong highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT ongkwokleung highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT cuestatorresluisaf highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT liuyifen highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT adamsafwaan highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT iqbalzohaib highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT dhageshaishav highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT ammoribasilj highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT syedakheela highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT ryekerryanne highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT tabetfatiha highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity
AT soranhandrean highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity