Cargando…
High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity
Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight los...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010476/ https://www.ncbi.nlm.nih.gov/pubmed/33093236 http://dx.doi.org/10.1194/jlr.RA120000963 |
_version_ | 1783673072831692800 |
---|---|
author | Ho, Jan Hoong Ong, Kwok Leung Cuesta Torres, Luisa F. Liu, Yifen Adam, Safwaan Iqbal, Zohaib Dhage, Shaishav Ammori, Basil J. Syed, Akheel A. Rye, Kerry-Anne Tabet, Fatiha Soran, Handrean |
author_facet | Ho, Jan Hoong Ong, Kwok Leung Cuesta Torres, Luisa F. Liu, Yifen Adam, Safwaan Iqbal, Zohaib Dhage, Shaishav Ammori, Basil J. Syed, Akheel A. Rye, Kerry-Anne Tabet, Fatiha Soran, Handrean |
author_sort | Ho, Jan Hoong |
collection | PubMed |
description | Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDLs were isolated from patients with severe obesity (n = 53) before and 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDLs were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222, and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222, and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222, and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase activity were increased and intercellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with an increase in cholesterol efflux capacity (r = 0.326, P = 0.027 and r = 0.349, P = 0.017, respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy participants versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction and may lead to the development of novel treatment strategies for obesity and related metabolic disorders. |
format | Online Article Text |
id | pubmed-8010476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-80104762021-04-02 High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity Ho, Jan Hoong Ong, Kwok Leung Cuesta Torres, Luisa F. Liu, Yifen Adam, Safwaan Iqbal, Zohaib Dhage, Shaishav Ammori, Basil J. Syed, Akheel A. Rye, Kerry-Anne Tabet, Fatiha Soran, Handrean J Lipid Res Research Article Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDLs were isolated from patients with severe obesity (n = 53) before and 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDLs were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222, and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222, and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222, and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase activity were increased and intercellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with an increase in cholesterol efflux capacity (r = 0.326, P = 0.027 and r = 0.349, P = 0.017, respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy participants versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction and may lead to the development of novel treatment strategies for obesity and related metabolic disorders. American Society for Biochemistry and Molecular Biology 2021-02-06 /pmc/articles/PMC8010476/ /pubmed/33093236 http://dx.doi.org/10.1194/jlr.RA120000963 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Ho, Jan Hoong Ong, Kwok Leung Cuesta Torres, Luisa F. Liu, Yifen Adam, Safwaan Iqbal, Zohaib Dhage, Shaishav Ammori, Basil J. Syed, Akheel A. Rye, Kerry-Anne Tabet, Fatiha Soran, Handrean High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity |
title | High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity |
title_full | High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity |
title_fullStr | High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity |
title_full_unstemmed | High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity |
title_short | High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity |
title_sort | high density lipoprotein-associated mirna is increased following roux-en-y gastric bypass surgery for severe obesity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010476/ https://www.ncbi.nlm.nih.gov/pubmed/33093236 http://dx.doi.org/10.1194/jlr.RA120000963 |
work_keys_str_mv | AT hojanhoong highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT ongkwokleung highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT cuestatorresluisaf highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT liuyifen highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT adamsafwaan highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT iqbalzohaib highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT dhageshaishav highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT ammoribasilj highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT syedakheela highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT ryekerryanne highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT tabetfatiha highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity AT soranhandrean highdensitylipoproteinassociatedmirnaisincreasedfollowingrouxenygastricbypasssurgeryforsevereobesity |