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Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity

Exosomes are small extracellular vesicles that are released from cells and that function in intercellular communication. Recently, interferon-inducible transmembrane protein 3 (IFITM3) has been identified as a highly effective anti-intracellular pathogen protein that can inhibit the invasion of a wi...

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Autores principales: Yi, Jihai, Wang, Yueli, Zhang, Huan, Deng, Xiaoyu, Xi, Jing, Li, Honghuan, Yang, Ningning, Ma, Zhongchen, Wang, Yong, Chen, Chuangfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010673/
https://www.ncbi.nlm.nih.gov/pubmed/33816587
http://dx.doi.org/10.3389/fvets.2021.642968
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author Yi, Jihai
Wang, Yueli
Zhang, Huan
Deng, Xiaoyu
Xi, Jing
Li, Honghuan
Yang, Ningning
Ma, Zhongchen
Wang, Yong
Chen, Chuangfu
author_facet Yi, Jihai
Wang, Yueli
Zhang, Huan
Deng, Xiaoyu
Xi, Jing
Li, Honghuan
Yang, Ningning
Ma, Zhongchen
Wang, Yong
Chen, Chuangfu
author_sort Yi, Jihai
collection PubMed
description Exosomes are small extracellular vesicles that are released from cells and that function in intercellular communication. Recently, interferon-inducible transmembrane protein 3 (IFITM3) has been identified as a highly effective anti-intracellular pathogen protein that can inhibit the invasion of a wide range of pathogenic microorganisms. However, whether Brucella infection induces secretion of exosomes and whether these exosomes contain IFITM3 protein remain unknown. Here, we focused on the immune function of extracellular IFITM3 protein in the process of Brucella infection. This study is the first to show that Brucella melitensis strain M5 (Brucella M5) can stimulate macrophages to secrete large amounts of exosomes. Most importantly, we identified exosomes from Brucella M5-infected cells that were rich in molecules of IFITM3, and these exosomes could transmit the IFITM3 from one cell to another, thereby effectively inhibiting the intracellular survival of Brucella. Moreover, immunization with exosomes carrying IFITM3 decreased mouse spleen tissue damage and spleen colony forming unit (CFU), leading to the establishment of an anti-Brucella state in mice. In conclusion, our findings provide new insights into the anti-Brucella mechanism of IFITM3-containg exosomes, thus providing a theoretical foundation for systematic elaboration of the mechanisms of Brucella infection and host immunity. The results provide new ideas for the development of candidate vaccines for Brucella.
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spelling pubmed-80106732021-04-01 Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity Yi, Jihai Wang, Yueli Zhang, Huan Deng, Xiaoyu Xi, Jing Li, Honghuan Yang, Ningning Ma, Zhongchen Wang, Yong Chen, Chuangfu Front Vet Sci Veterinary Science Exosomes are small extracellular vesicles that are released from cells and that function in intercellular communication. Recently, interferon-inducible transmembrane protein 3 (IFITM3) has been identified as a highly effective anti-intracellular pathogen protein that can inhibit the invasion of a wide range of pathogenic microorganisms. However, whether Brucella infection induces secretion of exosomes and whether these exosomes contain IFITM3 protein remain unknown. Here, we focused on the immune function of extracellular IFITM3 protein in the process of Brucella infection. This study is the first to show that Brucella melitensis strain M5 (Brucella M5) can stimulate macrophages to secrete large amounts of exosomes. Most importantly, we identified exosomes from Brucella M5-infected cells that were rich in molecules of IFITM3, and these exosomes could transmit the IFITM3 from one cell to another, thereby effectively inhibiting the intracellular survival of Brucella. Moreover, immunization with exosomes carrying IFITM3 decreased mouse spleen tissue damage and spleen colony forming unit (CFU), leading to the establishment of an anti-Brucella state in mice. In conclusion, our findings provide new insights into the anti-Brucella mechanism of IFITM3-containg exosomes, thus providing a theoretical foundation for systematic elaboration of the mechanisms of Brucella infection and host immunity. The results provide new ideas for the development of candidate vaccines for Brucella. Frontiers Media S.A. 2021-03-16 /pmc/articles/PMC8010673/ /pubmed/33816587 http://dx.doi.org/10.3389/fvets.2021.642968 Text en Copyright © 2021 Yi, Wang, Zhang, Deng, Xi, Li, Yang, Ma, Wang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Yi, Jihai
Wang, Yueli
Zhang, Huan
Deng, Xiaoyu
Xi, Jing
Li, Honghuan
Yang, Ningning
Ma, Zhongchen
Wang, Yong
Chen, Chuangfu
Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity
title Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity
title_full Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity
title_fullStr Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity
title_full_unstemmed Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity
title_short Interferon-Inducible Transmembrane Protein 3-Containing Exosome as a New Carrier for the Cell-to-Cell Transmission of Anti-Brucella Activity
title_sort interferon-inducible transmembrane protein 3-containing exosome as a new carrier for the cell-to-cell transmission of anti-brucella activity
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010673/
https://www.ncbi.nlm.nih.gov/pubmed/33816587
http://dx.doi.org/10.3389/fvets.2021.642968
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