Cargando…

Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7

Heat stress can decrease poultry performance indices, immune function, and intestinal development, which can reduce birds' innate protective mechanisms and may be more susceptible for pathogens. Ma chickens heat-stressed with 41°C for 12 h and recovered for 7 d had extremely low immunity. In th...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Lu-Ping, Li, Wei-Hao, Liu, Yi-Lei, Lun, Jian-Chi, He, Yong-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010704/
https://www.ncbi.nlm.nih.gov/pubmed/33752066
http://dx.doi.org/10.1016/j.psj.2021.101030
_version_ 1783673112180555776
author Tang, Lu-Ping
Li, Wei-Hao
Liu, Yi-Lei
Lun, Jian-Chi
He, Yong-Ming
author_facet Tang, Lu-Ping
Li, Wei-Hao
Liu, Yi-Lei
Lun, Jian-Chi
He, Yong-Ming
author_sort Tang, Lu-Ping
collection PubMed
description Heat stress can decrease poultry performance indices, immune function, and intestinal development, which can reduce birds' innate protective mechanisms and may be more susceptible for pathogens. Ma chickens heat-stressed with 41°C for 12 h and recovered for 7 d had extremely low immunity. In this study, a susceptible chicken model induced by heat stress and then infected with Escherichia coli O157:H7 was established to explore the mechanisms of birds' intestinal immune function changes. Ma chickens in heat stress + E. coli (HS + E. coli) group were stressed at 41°C for 12 h and recovered for 7 d, then chickens in E. coli group and HS + E. coli group were orally administered with 1 mL E. coli O157:H7 (1 × 10(9) cfu/mL). Chickens were sacrificed at the fourth day after E. coli administration. Results showed that the HS + E. coli group had increased intestinal length and weight, had higher E. coli counts in cecum contents than the E. coli group. Heat stress also enhanced serum diamine oxidase and decreased IgA level in chickens infected by E. coli. Heat stress had protective effects in small intestinal morphology except for duodenum by using hematoxylin and eosin staining. Compared with the E. coli group birds, IL-1β, TNF-α, and caspase-1 protein levels in the duodenum and ileum were significantly increased. Heat stress also can significantly enhance the gene and protein expression of Hsp70, TLR4, and NF-κB in the duodenum and ileum, respectively. The gene expression of Hsp70, TLR4, and NF-κB in the jejunum was not influenced, but the protein expression of Hsp70 and NF-κB was inhibited by heat stress. The results indicated heat stress can amplify the effect of E. coli on intestinal inflammatory injury of Ma chickens through increasing TLR4-NF-κB signaling pathway.
format Online
Article
Text
id pubmed-8010704
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-80107042021-04-02 Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7 Tang, Lu-Ping Li, Wei-Hao Liu, Yi-Lei Lun, Jian-Chi He, Yong-Ming Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Heat stress can decrease poultry performance indices, immune function, and intestinal development, which can reduce birds' innate protective mechanisms and may be more susceptible for pathogens. Ma chickens heat-stressed with 41°C for 12 h and recovered for 7 d had extremely low immunity. In this study, a susceptible chicken model induced by heat stress and then infected with Escherichia coli O157:H7 was established to explore the mechanisms of birds' intestinal immune function changes. Ma chickens in heat stress + E. coli (HS + E. coli) group were stressed at 41°C for 12 h and recovered for 7 d, then chickens in E. coli group and HS + E. coli group were orally administered with 1 mL E. coli O157:H7 (1 × 10(9) cfu/mL). Chickens were sacrificed at the fourth day after E. coli administration. Results showed that the HS + E. coli group had increased intestinal length and weight, had higher E. coli counts in cecum contents than the E. coli group. Heat stress also enhanced serum diamine oxidase and decreased IgA level in chickens infected by E. coli. Heat stress had protective effects in small intestinal morphology except for duodenum by using hematoxylin and eosin staining. Compared with the E. coli group birds, IL-1β, TNF-α, and caspase-1 protein levels in the duodenum and ileum were significantly increased. Heat stress also can significantly enhance the gene and protein expression of Hsp70, TLR4, and NF-κB in the duodenum and ileum, respectively. The gene expression of Hsp70, TLR4, and NF-κB in the jejunum was not influenced, but the protein expression of Hsp70 and NF-κB was inhibited by heat stress. The results indicated heat stress can amplify the effect of E. coli on intestinal inflammatory injury of Ma chickens through increasing TLR4-NF-κB signaling pathway. Elsevier 2021-01-28 /pmc/articles/PMC8010704/ /pubmed/33752066 http://dx.doi.org/10.1016/j.psj.2021.101030 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Tang, Lu-Ping
Li, Wei-Hao
Liu, Yi-Lei
Lun, Jian-Chi
He, Yong-Ming
Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7
title Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7
title_full Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7
title_fullStr Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7
title_full_unstemmed Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7
title_short Heat stress aggravates intestinal inflammation through TLR4-NF-κB signaling pathway in Ma chickens infected with Escherichia coli O157:H7
title_sort heat stress aggravates intestinal inflammation through tlr4-nf-κb signaling pathway in ma chickens infected with escherichia coli o157:h7
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010704/
https://www.ncbi.nlm.nih.gov/pubmed/33752066
http://dx.doi.org/10.1016/j.psj.2021.101030
work_keys_str_mv AT tangluping heatstressaggravatesintestinalinflammationthroughtlr4nfkbsignalingpathwayinmachickensinfectedwithescherichiacolio157h7
AT liweihao heatstressaggravatesintestinalinflammationthroughtlr4nfkbsignalingpathwayinmachickensinfectedwithescherichiacolio157h7
AT liuyilei heatstressaggravatesintestinalinflammationthroughtlr4nfkbsignalingpathwayinmachickensinfectedwithescherichiacolio157h7
AT lunjianchi heatstressaggravatesintestinalinflammationthroughtlr4nfkbsignalingpathwayinmachickensinfectedwithescherichiacolio157h7
AT heyongming heatstressaggravatesintestinalinflammationthroughtlr4nfkbsignalingpathwayinmachickensinfectedwithescherichiacolio157h7