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Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells
BACKGROUND: Vascular endothelial cells (ECs) are subject to continuous shear stress due to blood circulation. Mechanical stress due to high shear flow can also cause arteriovenous malformation (AVM) when ECs respond hyper-sensitively to shear flow. This study was conducted to test the hypothesis tha...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011119/ https://www.ncbi.nlm.nih.gov/pubmed/33789563 http://dx.doi.org/10.1186/s10020-021-00291-6 |
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author | Ryu, Jeong Yeop Kim, Yun Hyun Lee, Joon Seok Lee, Jeong Woo Oh, Eun Jung Kim, Hyun Mi Lee, Seok-Jong Lee, Jongmin Lee, Sang Yub Huh, Seung Kim, Ji Yoon Im, Saewon Chung, Ho Yun |
author_facet | Ryu, Jeong Yeop Kim, Yun Hyun Lee, Joon Seok Lee, Jeong Woo Oh, Eun Jung Kim, Hyun Mi Lee, Seok-Jong Lee, Jongmin Lee, Sang Yub Huh, Seung Kim, Ji Yoon Im, Saewon Chung, Ho Yun |
author_sort | Ryu, Jeong Yeop |
collection | PubMed |
description | BACKGROUND: Vascular endothelial cells (ECs) are subject to continuous shear stress due to blood circulation. Mechanical stress due to high shear flow can also cause arteriovenous malformation (AVM) when ECs respond hyper-sensitively to shear flow. This study was conducted to test the hypothesis that angiogenesis could be promoted in response to mechanical stress via regulation of pro-angiogenic factors in AVM cells. METHODS: ECs were extracted from the tissue samples from six AVM patients and six normal patients. Shear stress at 7 dynes/cm2 were applied for 24 h. Before and after application of shear stress to each group, RT-PCR was performed to access the expression levels of angiopoietin2(AGP2), aquaporin1(AQP1) and TGFβR1. Immunofluorescences was also performed to evaluate the level of protein expressions. RESULTS: In both normal and AVM tissues, AGP2 and TGFβR1 under the shear stress showed increased expression in the ECs compared to the non-sheared samples. When AVMs and normal arterial vasculature were compared, the expression levels of both AGP2 and TGFβR1 in AVMs were higher when compared to normal arterial vasculature with or without shear stress. Immunofluorescence-based protein analysis also confirmed shear-induced AGP2 and TGFβR1 in both samples of normal and AVM patients. CONCLUSIONS: AVMs exhibited higher sensitivity to shear stress by producing higher expressions of some marked genes and proteins that regulate the endothelial functions upon exposure to shear stress. While the physiological mechanism for AVMs remain elusive, our study shows the plausibility of physical stress imposed by the shearing flow can cause the occurrence of AVMs. |
format | Online Article Text |
id | pubmed-8011119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80111192021-03-31 Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells Ryu, Jeong Yeop Kim, Yun Hyun Lee, Joon Seok Lee, Jeong Woo Oh, Eun Jung Kim, Hyun Mi Lee, Seok-Jong Lee, Jongmin Lee, Sang Yub Huh, Seung Kim, Ji Yoon Im, Saewon Chung, Ho Yun Mol Med Research Article BACKGROUND: Vascular endothelial cells (ECs) are subject to continuous shear stress due to blood circulation. Mechanical stress due to high shear flow can also cause arteriovenous malformation (AVM) when ECs respond hyper-sensitively to shear flow. This study was conducted to test the hypothesis that angiogenesis could be promoted in response to mechanical stress via regulation of pro-angiogenic factors in AVM cells. METHODS: ECs were extracted from the tissue samples from six AVM patients and six normal patients. Shear stress at 7 dynes/cm2 were applied for 24 h. Before and after application of shear stress to each group, RT-PCR was performed to access the expression levels of angiopoietin2(AGP2), aquaporin1(AQP1) and TGFβR1. Immunofluorescences was also performed to evaluate the level of protein expressions. RESULTS: In both normal and AVM tissues, AGP2 and TGFβR1 under the shear stress showed increased expression in the ECs compared to the non-sheared samples. When AVMs and normal arterial vasculature were compared, the expression levels of both AGP2 and TGFβR1 in AVMs were higher when compared to normal arterial vasculature with or without shear stress. Immunofluorescence-based protein analysis also confirmed shear-induced AGP2 and TGFβR1 in both samples of normal and AVM patients. CONCLUSIONS: AVMs exhibited higher sensitivity to shear stress by producing higher expressions of some marked genes and proteins that regulate the endothelial functions upon exposure to shear stress. While the physiological mechanism for AVMs remain elusive, our study shows the plausibility of physical stress imposed by the shearing flow can cause the occurrence of AVMs. BioMed Central 2021-03-31 /pmc/articles/PMC8011119/ /pubmed/33789563 http://dx.doi.org/10.1186/s10020-021-00291-6 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Ryu, Jeong Yeop Kim, Yun Hyun Lee, Joon Seok Lee, Jeong Woo Oh, Eun Jung Kim, Hyun Mi Lee, Seok-Jong Lee, Jongmin Lee, Sang Yub Huh, Seung Kim, Ji Yoon Im, Saewon Chung, Ho Yun Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
title | Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
title_full | Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
title_fullStr | Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
title_full_unstemmed | Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
title_short | Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
title_sort | oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011119/ https://www.ncbi.nlm.nih.gov/pubmed/33789563 http://dx.doi.org/10.1186/s10020-021-00291-6 |
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