Detection of gene cis-regulatory element perturbations in single-cell transcriptomes

We introduce poly-adenine CRISPR gRNA-based single-cell RNA-sequencing (pAC-Seq), a method that enables the direct observation of guide RNAs (gRNAs) in scRNA-seq. We use pAC-Seq to assess the phenotypic consequences of CRISPR/Cas9 based alterations of gene cis-regulatory regions. We show that pAC-Se...

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Detalles Bibliográficos
Autores principales: Yeo, Grace Hui Ting, Juez, Oscar, Chen, Qing, Banerjee, Budhaditya, Chu, Lendy, Shen, Max W., Sabry, May, Logister, Ive, Sherwood, Richard I., Gifford, David K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011753/
https://www.ncbi.nlm.nih.gov/pubmed/33711017
http://dx.doi.org/10.1371/journal.pcbi.1008789

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