Cargando…

Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis

Osteosarcoma (OS), also known as bone cancer, is a threat to the lives of millions of adolescents worldwide. Although dedicated efforts have been invested in reducing the mortality rate of this bone cancer, the research community is yet to find the exact causes of OS. Thus, the present research aime...

Descripción completa

Detalles Bibliográficos
Autores principales: Qin, Guanghua, Wu, Xuejian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012025/
https://www.ncbi.nlm.nih.gov/pubmed/33786605
http://dx.doi.org/10.3892/ijmm.2021.4925
_version_ 1783673300575059968
author Qin, Guanghua
Wu, Xuejian
author_facet Qin, Guanghua
Wu, Xuejian
author_sort Qin, Guanghua
collection PubMed
description Osteosarcoma (OS), also known as bone cancer, is a threat to the lives of millions of adolescents worldwide. Although dedicated efforts have been invested in reducing the mortality rate of this bone cancer, the research community is yet to find the exact causes of OS. Thus, the present research aimed to study the association between circular RNA circ_0032463 and OS progression. The impact of circ_0032463 on cells with OS was first evaluated using reverse transcription-quantitative PCR. This evaluation was followed by the assessment of cell proliferation, viability, apoptosis, invasion and adhesion using BrdU, Cell Counting Kit-8, flow cytometry, Transwell and cell adhesion assays, respectively. RNA pull-down, RNA immunoprecipitation chip and dual-luciferase reporter systems were utilized to investigate the relationship between circ_0032463, microRNA (miR)-330-3p and Pinin desmosome associated protein (PNN) in OS. The findings indicated that circ_0032463 and PNN were highly expressed in OS tissues and OS cell lines, and that they facilitated cell proliferation, viability, invasion and adhesion, but attenuated cell apoptosis in OS cells. The low expression of miR-330-3p suppressed OS development. It was also noted that circ_0032463 inhibited miR-330-3p to upregulate PNN expression. In conclusion, this study confirmed that by regulating the miR-330-3p/PNN axis, circular RNA circ_0032463 could function as a tumor enhancer in cells with OS.
format Online
Article
Text
id pubmed-8012025
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-80120252021-04-01 Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis Qin, Guanghua Wu, Xuejian Int J Mol Med Articles Osteosarcoma (OS), also known as bone cancer, is a threat to the lives of millions of adolescents worldwide. Although dedicated efforts have been invested in reducing the mortality rate of this bone cancer, the research community is yet to find the exact causes of OS. Thus, the present research aimed to study the association between circular RNA circ_0032463 and OS progression. The impact of circ_0032463 on cells with OS was first evaluated using reverse transcription-quantitative PCR. This evaluation was followed by the assessment of cell proliferation, viability, apoptosis, invasion and adhesion using BrdU, Cell Counting Kit-8, flow cytometry, Transwell and cell adhesion assays, respectively. RNA pull-down, RNA immunoprecipitation chip and dual-luciferase reporter systems were utilized to investigate the relationship between circ_0032463, microRNA (miR)-330-3p and Pinin desmosome associated protein (PNN) in OS. The findings indicated that circ_0032463 and PNN were highly expressed in OS tissues and OS cell lines, and that they facilitated cell proliferation, viability, invasion and adhesion, but attenuated cell apoptosis in OS cells. The low expression of miR-330-3p suppressed OS development. It was also noted that circ_0032463 inhibited miR-330-3p to upregulate PNN expression. In conclusion, this study confirmed that by regulating the miR-330-3p/PNN axis, circular RNA circ_0032463 could function as a tumor enhancer in cells with OS. D.A. Spandidos 2021-05 2021-03-30 /pmc/articles/PMC8012025/ /pubmed/33786605 http://dx.doi.org/10.3892/ijmm.2021.4925 Text en Copyright: © Qin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Qin, Guanghua
Wu, Xuejian
Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis
title Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis
title_full Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis
title_fullStr Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis
title_full_unstemmed Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis
title_short Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR-330-3p/PNN axis
title_sort hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the mir-330-3p/pnn axis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012025/
https://www.ncbi.nlm.nih.gov/pubmed/33786605
http://dx.doi.org/10.3892/ijmm.2021.4925
work_keys_str_mv AT qinguanghua hsacirc0032463actsasthetumorpromoterinosteosarcomabyregulatingthemir3303ppnnaxis
AT wuxuejian hsacirc0032463actsasthetumorpromoterinosteosarcomabyregulatingthemir3303ppnnaxis