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HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase
Poly(ADP-ribose) polymerase 1 (PARP1) is an important player in the response to DNA damage. Recently, Histone PARylation Factor (HPF1) was shown to be a critical modulator of the activity of PARP1 by facilitating PARylation of histones and redirecting the target amino acid specificity from acidic to...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012059/ https://www.ncbi.nlm.nih.gov/pubmed/33683197 http://dx.doi.org/10.7554/eLife.65773 |
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author | Rudolph, Johannes Roberts, Genevieve Muthurajan, Uma M Luger, Karolin |
author_facet | Rudolph, Johannes Roberts, Genevieve Muthurajan, Uma M Luger, Karolin |
author_sort | Rudolph, Johannes |
collection | PubMed |
description | Poly(ADP-ribose) polymerase 1 (PARP1) is an important player in the response to DNA damage. Recently, Histone PARylation Factor (HPF1) was shown to be a critical modulator of the activity of PARP1 by facilitating PARylation of histones and redirecting the target amino acid specificity from acidic to serine residues. Here, we investigate the mechanism and specific consequences of HPF1-mediated PARylation using nucleosomes as both activators and substrates for PARP1. HPF1 provides that catalytic base Glu284 to substantially redirect PARylation by PARP1 such that the histones in nucleosomes become the primary recipients of PAR chains. Surprisingly, HPF1 partitions most of the reaction product to free ADP-ribose (ADPR), resulting in much shorter PAR chains compared to reactions in the absence of HPF1. This HPF1-mediated switch from polymerase to hydrolase has important implications for the PARP1-mediated response to DNA damage and raises interesting new questions about the role of intracellular ADPR and depletion of NAD(+). |
format | Online Article Text |
id | pubmed-8012059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80120592021-04-02 HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase Rudolph, Johannes Roberts, Genevieve Muthurajan, Uma M Luger, Karolin eLife Biochemistry and Chemical Biology Poly(ADP-ribose) polymerase 1 (PARP1) is an important player in the response to DNA damage. Recently, Histone PARylation Factor (HPF1) was shown to be a critical modulator of the activity of PARP1 by facilitating PARylation of histones and redirecting the target amino acid specificity from acidic to serine residues. Here, we investigate the mechanism and specific consequences of HPF1-mediated PARylation using nucleosomes as both activators and substrates for PARP1. HPF1 provides that catalytic base Glu284 to substantially redirect PARylation by PARP1 such that the histones in nucleosomes become the primary recipients of PAR chains. Surprisingly, HPF1 partitions most of the reaction product to free ADP-ribose (ADPR), resulting in much shorter PAR chains compared to reactions in the absence of HPF1. This HPF1-mediated switch from polymerase to hydrolase has important implications for the PARP1-mediated response to DNA damage and raises interesting new questions about the role of intracellular ADPR and depletion of NAD(+). eLife Sciences Publications, Ltd 2021-03-08 /pmc/articles/PMC8012059/ /pubmed/33683197 http://dx.doi.org/10.7554/eLife.65773 Text en © 2021, Rudolph et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Rudolph, Johannes Roberts, Genevieve Muthurajan, Uma M Luger, Karolin HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase |
title | HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase |
title_full | HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase |
title_fullStr | HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase |
title_full_unstemmed | HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase |
title_short | HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase |
title_sort | hpf1 and nucleosomes mediate a dramatic switch in activity of parp1 from polymerase to hydrolase |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012059/ https://www.ncbi.nlm.nih.gov/pubmed/33683197 http://dx.doi.org/10.7554/eLife.65773 |
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