CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum

The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum. However, the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive. In this study, we revealed that RADICAL-INDUCED CE...

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Autores principales: Wang, Lijun, Cheng, Hua, Wang, Qi, Si, Chaona, Yang, Yiman, Yu, Yao, Zhou, Lijie, Ding, Lian, Song, Aiping, Xu, Dongqing, Chen, Sumei, Fang, Weimin, Chen, Fadi, Jiang, Jiafu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012346/
https://www.ncbi.nlm.nih.gov/pubmed/33790241
http://dx.doi.org/10.1038/s41438-021-00516-z
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author Wang, Lijun
Cheng, Hua
Wang, Qi
Si, Chaona
Yang, Yiman
Yu, Yao
Zhou, Lijie
Ding, Lian
Song, Aiping
Xu, Dongqing
Chen, Sumei
Fang, Weimin
Chen, Fadi
Jiang, Jiafu
author_facet Wang, Lijun
Cheng, Hua
Wang, Qi
Si, Chaona
Yang, Yiman
Yu, Yao
Zhou, Lijie
Ding, Lian
Song, Aiping
Xu, Dongqing
Chen, Sumei
Fang, Weimin
Chen, Fadi
Jiang, Jiafu
author_sort Wang, Lijun
collection PubMed
description The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum. However, the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive. In this study, we revealed that RADICAL-INDUCED CELL DEATH 1 (CmRCD1) physically associated with CmBBX8 through bimolecular fluorescence complementation (BiFC), pulldown and Coimmunoprecipitation (CoIP) assays. Furthermore, the RCD1-SRO1-TAF4 (RST) domain of CmRCD1 and the B-box of CmBBX8 mediated their interaction. In addition, Luciferase (LUC) assays and electrophoretic mobility shift assay (EMSAs) showed that CmRCD1 repressed the transcriptional activity of CmBBX8 and interfered with its binding to the CmFTL1 promoter, thereby leading to delayed flowering in the summer chrysanthemum ‘Yuuka’. These results provide insight into the molecular framework of CmRCD1-CmBBX8-mediated flowering in chrysanthemum.
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spelling pubmed-80123462021-04-16 CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum Wang, Lijun Cheng, Hua Wang, Qi Si, Chaona Yang, Yiman Yu, Yao Zhou, Lijie Ding, Lian Song, Aiping Xu, Dongqing Chen, Sumei Fang, Weimin Chen, Fadi Jiang, Jiafu Hortic Res Article The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum. However, the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive. In this study, we revealed that RADICAL-INDUCED CELL DEATH 1 (CmRCD1) physically associated with CmBBX8 through bimolecular fluorescence complementation (BiFC), pulldown and Coimmunoprecipitation (CoIP) assays. Furthermore, the RCD1-SRO1-TAF4 (RST) domain of CmRCD1 and the B-box of CmBBX8 mediated their interaction. In addition, Luciferase (LUC) assays and electrophoretic mobility shift assay (EMSAs) showed that CmRCD1 repressed the transcriptional activity of CmBBX8 and interfered with its binding to the CmFTL1 promoter, thereby leading to delayed flowering in the summer chrysanthemum ‘Yuuka’. These results provide insight into the molecular framework of CmRCD1-CmBBX8-mediated flowering in chrysanthemum. Nature Publishing Group UK 2021-04-01 /pmc/articles/PMC8012346/ /pubmed/33790241 http://dx.doi.org/10.1038/s41438-021-00516-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Lijun
Cheng, Hua
Wang, Qi
Si, Chaona
Yang, Yiman
Yu, Yao
Zhou, Lijie
Ding, Lian
Song, Aiping
Xu, Dongqing
Chen, Sumei
Fang, Weimin
Chen, Fadi
Jiang, Jiafu
CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
title CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
title_full CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
title_fullStr CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
title_full_unstemmed CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
title_short CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
title_sort cmrcd1 represses flowering by directly interacting with cmbbx8 in summer chrysanthemum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012346/
https://www.ncbi.nlm.nih.gov/pubmed/33790241
http://dx.doi.org/10.1038/s41438-021-00516-z
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