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Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission
Abscission, a cell separation process, is an important trait that influences grain and fruit yield. We previously reported that BEL1-LIKE HOMEODOMAIN 4 (SlBL4) is involved in chloroplast development and cell wall metabolism in tomato fruit. In the present study, we showed that silencing SlBL4 result...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012377/ https://www.ncbi.nlm.nih.gov/pubmed/33790250 http://dx.doi.org/10.1038/s41438-021-00515-0 |
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author | Yan, Fang Gong, Zhehao Hu, Guojian Ma, Xuesong Bai, Runyao Yu, Ruonan Zhang, Qiang Deng, Wei Li, Zhengguo Wuriyanghan, Hada |
author_facet | Yan, Fang Gong, Zhehao Hu, Guojian Ma, Xuesong Bai, Runyao Yu, Ruonan Zhang, Qiang Deng, Wei Li, Zhengguo Wuriyanghan, Hada |
author_sort | Yan, Fang |
collection | PubMed |
description | Abscission, a cell separation process, is an important trait that influences grain and fruit yield. We previously reported that BEL1-LIKE HOMEODOMAIN 4 (SlBL4) is involved in chloroplast development and cell wall metabolism in tomato fruit. In the present study, we showed that silencing SlBL4 resulted in the enlargement and pre-abscission of the tomato (Solanum lycopersicum cv. Micro-TOM) fruit pedicel. The anatomic analysis showed the presence of more epidermal cell layers and no obvious abscission zone (AZ) in the SlBL4 RNAi lines compared with the wild-type plants. RNA-seq analysis indicated that the regulation of abscission by SlBL4 was associated with the altered abundance of genes related to key meristems, auxin transporters, signaling components, and cell wall metabolism. Furthermore, SlBL4 positively affected the auxin concentration in the abscission zone. A dual-luciferase reporter assay revealed that SlBL4 activated the transcription of the JOINTLESS, OVATE, PIN1, and LAX3 genes. We reported a novel function of SlBL4, which plays key roles in fruit pedicel organogenesis and abscission in tomatoes. |
format | Online Article Text |
id | pubmed-8012377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80123772021-04-16 Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission Yan, Fang Gong, Zhehao Hu, Guojian Ma, Xuesong Bai, Runyao Yu, Ruonan Zhang, Qiang Deng, Wei Li, Zhengguo Wuriyanghan, Hada Hortic Res Article Abscission, a cell separation process, is an important trait that influences grain and fruit yield. We previously reported that BEL1-LIKE HOMEODOMAIN 4 (SlBL4) is involved in chloroplast development and cell wall metabolism in tomato fruit. In the present study, we showed that silencing SlBL4 resulted in the enlargement and pre-abscission of the tomato (Solanum lycopersicum cv. Micro-TOM) fruit pedicel. The anatomic analysis showed the presence of more epidermal cell layers and no obvious abscission zone (AZ) in the SlBL4 RNAi lines compared with the wild-type plants. RNA-seq analysis indicated that the regulation of abscission by SlBL4 was associated with the altered abundance of genes related to key meristems, auxin transporters, signaling components, and cell wall metabolism. Furthermore, SlBL4 positively affected the auxin concentration in the abscission zone. A dual-luciferase reporter assay revealed that SlBL4 activated the transcription of the JOINTLESS, OVATE, PIN1, and LAX3 genes. We reported a novel function of SlBL4, which plays key roles in fruit pedicel organogenesis and abscission in tomatoes. Nature Publishing Group UK 2021-04-01 /pmc/articles/PMC8012377/ /pubmed/33790250 http://dx.doi.org/10.1038/s41438-021-00515-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yan, Fang Gong, Zhehao Hu, Guojian Ma, Xuesong Bai, Runyao Yu, Ruonan Zhang, Qiang Deng, Wei Li, Zhengguo Wuriyanghan, Hada Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission |
title | Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission |
title_full | Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission |
title_fullStr | Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission |
title_full_unstemmed | Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission |
title_short | Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission |
title_sort | tomato slbl4 plays an important role in fruit pedicel organogenesis and abscission |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012377/ https://www.ncbi.nlm.nih.gov/pubmed/33790250 http://dx.doi.org/10.1038/s41438-021-00515-0 |
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