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(18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats

A majority of blast-induced mild traumatic brain injury (mTBI) patients experience persistent neurological dysfunction with no findings on conventional structural MR imaging. It is urgent to develop advanced imaging modalities to detect and understand the pathophysiology of blast-induced mTBI. Fluor...

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Autores principales: Li, Yang, Liu, Kaijun, Li, Chang, Guo, Yu, Fang, Jingqin, Tong, Haipeng, Tang, Yi, Zhang, Junfeng, Sun, Jinju, Jiao, Fangyang, Zhang, Qianhui, Jin, Rongbing, Xiong, Kunlin, Chen, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012735/
https://www.ncbi.nlm.nih.gov/pubmed/33815036
http://dx.doi.org/10.3389/fnins.2021.593723
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author Li, Yang
Liu, Kaijun
Li, Chang
Guo, Yu
Fang, Jingqin
Tong, Haipeng
Tang, Yi
Zhang, Junfeng
Sun, Jinju
Jiao, Fangyang
Zhang, Qianhui
Jin, Rongbing
Xiong, Kunlin
Chen, Xiao
author_facet Li, Yang
Liu, Kaijun
Li, Chang
Guo, Yu
Fang, Jingqin
Tong, Haipeng
Tang, Yi
Zhang, Junfeng
Sun, Jinju
Jiao, Fangyang
Zhang, Qianhui
Jin, Rongbing
Xiong, Kunlin
Chen, Xiao
author_sort Li, Yang
collection PubMed
description A majority of blast-induced mild traumatic brain injury (mTBI) patients experience persistent neurological dysfunction with no findings on conventional structural MR imaging. It is urgent to develop advanced imaging modalities to detect and understand the pathophysiology of blast-induced mTBI. Fluorine-18 fluorodeoxyglucose positron emission tomography ((18)F-FDG PET) could detect neuronal function and activity of the injured brain, while MR spectroscopy provides complementary information and assesses metabolic irregularities following injury. This study aims to investigate the effectiveness of combining (18)F-FDG PET with MR spectroscopy to evaluate acute and subacute metabolic cerebral alterations caused by blast-induced mTBI. Thirty-two adult male Sprague–Dawley rats were exposed to a single blast (mTBI group) and 32 rats were not exposed to the blast (sham group), followed by (18)F-FDG PET, MRI, and histological evaluation at baseline, 1–3 h, 1 day, and 7 days post-injury in three separate cohorts. (18)F-FDG uptake showed a transient increase in the amygdala and somatosensory cortex, followed by a gradual return to baseline from day 1 to 7 days post-injury and a continuous rise in the motor cortex. In contrast, decreased (18)F-FDG uptake was seen in the midbrain structures (inferior and superior colliculus). Analysis of MR spectroscopy showed that inflammation marker myo-inositol (Ins), oxidative stress marker glutamine + glutamate (Glx), and hypoxia marker lactate (Lac) levels markedly elevated over time in the somatosensory cortex, while the major osmolyte taurine (Tau) level immediately increased at 1–3 h and 1 day, and then returned to sham level on 7 days post-injury, which could be due to the disruption of the blood–brain barrier. Increased (18)F-FDG uptake and elevated Ins and Glx levels over time were confirmed by histology analysis which showed increased microglial activation and gliosis in the frontal cortex. These results suggest that (18)F-FDG PET and MR spectroscopy can be used together to reflect more comprehensive neuropathological alterations in vivo, which could improve our understanding of the complex alterations in the brain after blast-induced mTBI.
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spelling pubmed-80127352021-04-02 (18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats Li, Yang Liu, Kaijun Li, Chang Guo, Yu Fang, Jingqin Tong, Haipeng Tang, Yi Zhang, Junfeng Sun, Jinju Jiao, Fangyang Zhang, Qianhui Jin, Rongbing Xiong, Kunlin Chen, Xiao Front Neurosci Neuroscience A majority of blast-induced mild traumatic brain injury (mTBI) patients experience persistent neurological dysfunction with no findings on conventional structural MR imaging. It is urgent to develop advanced imaging modalities to detect and understand the pathophysiology of blast-induced mTBI. Fluorine-18 fluorodeoxyglucose positron emission tomography ((18)F-FDG PET) could detect neuronal function and activity of the injured brain, while MR spectroscopy provides complementary information and assesses metabolic irregularities following injury. This study aims to investigate the effectiveness of combining (18)F-FDG PET with MR spectroscopy to evaluate acute and subacute metabolic cerebral alterations caused by blast-induced mTBI. Thirty-two adult male Sprague–Dawley rats were exposed to a single blast (mTBI group) and 32 rats were not exposed to the blast (sham group), followed by (18)F-FDG PET, MRI, and histological evaluation at baseline, 1–3 h, 1 day, and 7 days post-injury in three separate cohorts. (18)F-FDG uptake showed a transient increase in the amygdala and somatosensory cortex, followed by a gradual return to baseline from day 1 to 7 days post-injury and a continuous rise in the motor cortex. In contrast, decreased (18)F-FDG uptake was seen in the midbrain structures (inferior and superior colliculus). Analysis of MR spectroscopy showed that inflammation marker myo-inositol (Ins), oxidative stress marker glutamine + glutamate (Glx), and hypoxia marker lactate (Lac) levels markedly elevated over time in the somatosensory cortex, while the major osmolyte taurine (Tau) level immediately increased at 1–3 h and 1 day, and then returned to sham level on 7 days post-injury, which could be due to the disruption of the blood–brain barrier. Increased (18)F-FDG uptake and elevated Ins and Glx levels over time were confirmed by histology analysis which showed increased microglial activation and gliosis in the frontal cortex. These results suggest that (18)F-FDG PET and MR spectroscopy can be used together to reflect more comprehensive neuropathological alterations in vivo, which could improve our understanding of the complex alterations in the brain after blast-induced mTBI. Frontiers Media S.A. 2021-03-18 /pmc/articles/PMC8012735/ /pubmed/33815036 http://dx.doi.org/10.3389/fnins.2021.593723 Text en Copyright © 2021 Li, Liu, Li, Guo, Fang, Tong, Tang, Zhang, Sun, Jiao, Zhang, Jin, Xiong and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Li, Yang
Liu, Kaijun
Li, Chang
Guo, Yu
Fang, Jingqin
Tong, Haipeng
Tang, Yi
Zhang, Junfeng
Sun, Jinju
Jiao, Fangyang
Zhang, Qianhui
Jin, Rongbing
Xiong, Kunlin
Chen, Xiao
(18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats
title (18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats
title_full (18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats
title_fullStr (18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats
title_full_unstemmed (18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats
title_short (18)F-FDG PET Combined With MR Spectroscopy Elucidates the Progressive Metabolic Cerebral Alterations After Blast-Induced Mild Traumatic Brain Injury in Rats
title_sort (18)f-fdg pet combined with mr spectroscopy elucidates the progressive metabolic cerebral alterations after blast-induced mild traumatic brain injury in rats
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012735/
https://www.ncbi.nlm.nih.gov/pubmed/33815036
http://dx.doi.org/10.3389/fnins.2021.593723
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