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Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice

Constipation is a prevalent gastrointestinal disorder that seriously reduces the quality of life. Clinical studies have shown that a great change or severe imbalance occurs in the intestinal microbiota of people with constipation. This study explored whether bacteriocin-producing and non-bacteriocin...

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Autores principales: Qiao, Yiteng, Qiu, Zhichang, Tian, Fengwei, Yu, Leilei, Zhao, Jianxin, Zhang, Hao, Zhai, Qixiao, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012752/
https://www.ncbi.nlm.nih.gov/pubmed/33816357
http://dx.doi.org/10.3389/fcimb.2021.655258
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author Qiao, Yiteng
Qiu, Zhichang
Tian, Fengwei
Yu, Leilei
Zhao, Jianxin
Zhang, Hao
Zhai, Qixiao
Chen, Wei
author_facet Qiao, Yiteng
Qiu, Zhichang
Tian, Fengwei
Yu, Leilei
Zhao, Jianxin
Zhang, Hao
Zhai, Qixiao
Chen, Wei
author_sort Qiao, Yiteng
collection PubMed
description Constipation is a prevalent gastrointestinal disorder that seriously reduces the quality of life. Clinical studies have shown that a great change or severe imbalance occurs in the intestinal microbiota of people with constipation. This study explored whether bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains resulted in differences in the alleviation of constipation and changes in the fecal flora in BALB/c mice. The constipation-related indicators, gastrointestinal regulatory peptides and gut microbiota were identified to evaluate their alleviating effects and underlying mechanisms. The time to the first black-stool defecation and the gastrointestinal transit rate in constipated mice were found to be somewhat improved by four P. acidilactici strains (P > 0.05). Moreover, there were significant differences in the level of most gastrointestinal regulatory peptides in the serum, as well as in the composition and abundance of intestinal microbiota in different groups (P < 0.05). At the phylum level, the relative abundance of Firmicutes was significantly increased, but those of Bacteroidetes and Proteobacteria were significantly reduced after the administration of four P. acidilactici strains for 14 d (P < 0.05). The levels of Bacteroides and genera from Enterobacteriaceae were significantly decreased, whereas Bifidobacterium and Lactobacillus were upregulated when bacteriocin-producing P. acidilactici CCFM18 and CCFM28 strains were provided in the diet (P < 0.05). The results indicated that although constipation-related symptoms were alleviated to only a limited degree, the administration of four P. acidilactici strains effectively regulated the gut flora and provided a potential health benefit to the host, especially the bacteriocin-producing P. acidilactici strains.
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spelling pubmed-80127522021-04-02 Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice Qiao, Yiteng Qiu, Zhichang Tian, Fengwei Yu, Leilei Zhao, Jianxin Zhang, Hao Zhai, Qixiao Chen, Wei Front Cell Infect Microbiol Cellular and Infection Microbiology Constipation is a prevalent gastrointestinal disorder that seriously reduces the quality of life. Clinical studies have shown that a great change or severe imbalance occurs in the intestinal microbiota of people with constipation. This study explored whether bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains resulted in differences in the alleviation of constipation and changes in the fecal flora in BALB/c mice. The constipation-related indicators, gastrointestinal regulatory peptides and gut microbiota were identified to evaluate their alleviating effects and underlying mechanisms. The time to the first black-stool defecation and the gastrointestinal transit rate in constipated mice were found to be somewhat improved by four P. acidilactici strains (P > 0.05). Moreover, there were significant differences in the level of most gastrointestinal regulatory peptides in the serum, as well as in the composition and abundance of intestinal microbiota in different groups (P < 0.05). At the phylum level, the relative abundance of Firmicutes was significantly increased, but those of Bacteroidetes and Proteobacteria were significantly reduced after the administration of four P. acidilactici strains for 14 d (P < 0.05). The levels of Bacteroides and genera from Enterobacteriaceae were significantly decreased, whereas Bifidobacterium and Lactobacillus were upregulated when bacteriocin-producing P. acidilactici CCFM18 and CCFM28 strains were provided in the diet (P < 0.05). The results indicated that although constipation-related symptoms were alleviated to only a limited degree, the administration of four P. acidilactici strains effectively regulated the gut flora and provided a potential health benefit to the host, especially the bacteriocin-producing P. acidilactici strains. Frontiers Media S.A. 2021-03-18 /pmc/articles/PMC8012752/ /pubmed/33816357 http://dx.doi.org/10.3389/fcimb.2021.655258 Text en Copyright © 2021 Qiao, Qiu, Tian, Yu, Zhao, Zhang, Zhai and Chen http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Qiao, Yiteng
Qiu, Zhichang
Tian, Fengwei
Yu, Leilei
Zhao, Jianxin
Zhang, Hao
Zhai, Qixiao
Chen, Wei
Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice
title Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice
title_full Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice
title_fullStr Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice
title_full_unstemmed Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice
title_short Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice
title_sort pediococcus acidilactici strains improve constipation symptoms and regulate intestinal flora in mice
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012752/
https://www.ncbi.nlm.nih.gov/pubmed/33816357
http://dx.doi.org/10.3389/fcimb.2021.655258
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