Cargando…

Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma

Lung squamous cell carcinoma (LSCC) is one of the most common types of lung cancer in adults worldwide. With the development of modern medicine, cancer treatment that harnesses the power of the immune system might be particularly effective for treating LSCC. In this research, LSCC expression data, w...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Min, Li, Yukun, Luo, Xianyu, Xiao, Jiao, Wang, Juan, Zeng, Xin, Hu, Qihao, Chen, Xiaoyan, Tan, Si-jie, Hu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012856/
https://www.ncbi.nlm.nih.gov/pubmed/33816462
http://dx.doi.org/10.3389/fcell.2021.606106
_version_ 1783673451971608576
author Tang, Min
Li, Yukun
Luo, Xianyu
Xiao, Jiao
Wang, Juan
Zeng, Xin
Hu, Qihao
Chen, Xiaoyan
Tan, Si-jie
Hu, Jun
author_facet Tang, Min
Li, Yukun
Luo, Xianyu
Xiao, Jiao
Wang, Juan
Zeng, Xin
Hu, Qihao
Chen, Xiaoyan
Tan, Si-jie
Hu, Jun
author_sort Tang, Min
collection PubMed
description Lung squamous cell carcinoma (LSCC) is one of the most common types of lung cancer in adults worldwide. With the development of modern medicine, cancer treatment that harnesses the power of the immune system might be particularly effective for treating LSCC. In this research, LSCC expression data, which quantify the cellular composition of immune cells, were analyzed by weighted gene coexpression network analysis (WGCNA) and a deconvolution algorithm based on the Gene Expression Omnibus (GEO) database, and the results indicated a close relationship between LSCC and CD8(+) T cells. Six hub genes (SYT3, METTL8, HSPB3, GFM1, ERLIN2, and CLCN2) were verified by gene–gene network and protein–protein interaction (PPI) network analyses. We found that the six hub genes were increased in cancer tissues and were closely correlated with cancer development and progression. After immune correlation analysis, METTL8 was selected as a prognostic biomarker. Finally, we found that the METTL8 levels were increased in multiple lung cancer cell lines and LSCC tissues. METTL8 inhibition could clearly induce G1 cell cycle arrest and suppress proliferation. Therefore, METTL8, which is related to CD8(+) T cell infiltration, might be identified as a potential biomarker and gene therapy target in LSCC.
format Online
Article
Text
id pubmed-8012856
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80128562021-04-02 Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma Tang, Min Li, Yukun Luo, Xianyu Xiao, Jiao Wang, Juan Zeng, Xin Hu, Qihao Chen, Xiaoyan Tan, Si-jie Hu, Jun Front Cell Dev Biol Cell and Developmental Biology Lung squamous cell carcinoma (LSCC) is one of the most common types of lung cancer in adults worldwide. With the development of modern medicine, cancer treatment that harnesses the power of the immune system might be particularly effective for treating LSCC. In this research, LSCC expression data, which quantify the cellular composition of immune cells, were analyzed by weighted gene coexpression network analysis (WGCNA) and a deconvolution algorithm based on the Gene Expression Omnibus (GEO) database, and the results indicated a close relationship between LSCC and CD8(+) T cells. Six hub genes (SYT3, METTL8, HSPB3, GFM1, ERLIN2, and CLCN2) were verified by gene–gene network and protein–protein interaction (PPI) network analyses. We found that the six hub genes were increased in cancer tissues and were closely correlated with cancer development and progression. After immune correlation analysis, METTL8 was selected as a prognostic biomarker. Finally, we found that the METTL8 levels were increased in multiple lung cancer cell lines and LSCC tissues. METTL8 inhibition could clearly induce G1 cell cycle arrest and suppress proliferation. Therefore, METTL8, which is related to CD8(+) T cell infiltration, might be identified as a potential biomarker and gene therapy target in LSCC. Frontiers Media S.A. 2021-03-18 /pmc/articles/PMC8012856/ /pubmed/33816462 http://dx.doi.org/10.3389/fcell.2021.606106 Text en Copyright © 2021 Tang, Li, Luo, Xiao, Wang, Zeng, Hu, Chen, Tan and Hu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Tang, Min
Li, Yukun
Luo, Xianyu
Xiao, Jiao
Wang, Juan
Zeng, Xin
Hu, Qihao
Chen, Xiaoyan
Tan, Si-jie
Hu, Jun
Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma
title Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma
title_full Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma
title_fullStr Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma
title_full_unstemmed Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma
title_short Identification of Biomarkers Related to CD8(+) T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma
title_sort identification of biomarkers related to cd8(+) t cell infiltration with gene co-expression network in lung squamous cell carcinoma
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012856/
https://www.ncbi.nlm.nih.gov/pubmed/33816462
http://dx.doi.org/10.3389/fcell.2021.606106
work_keys_str_mv AT tangmin identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT liyukun identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT luoxianyu identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT xiaojiao identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT wangjuan identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT zengxin identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT huqihao identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT chenxiaoyan identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT tansijie identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma
AT hujun identificationofbiomarkersrelatedtocd8tcellinfiltrationwithgenecoexpressionnetworkinlungsquamouscellcarcinoma