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The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes

Protein termini are determinants of protein stability. Proteins bearing degradation signals, or degrons, at their amino‐ or carboxyl‐termini are eliminated by the N‐ or C‐degron pathways, respectively. We aimed to elucidate the function of C‐degron pathways and to unveil how normal proteomes are exe...

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Autores principales: Yeh, Chi‐Wei, Huang, Wei‐Chieh, Hsu, Pang‐Hung, Yeh, Kun‐Hai, Wang, Li‐Chin, Hsu, Paul Wei‐Che, Lin, Hsiu‐Chuan, Chen, Yi‐Ning, Chen, Shu‐Chuan, Yeang, Chen‐Hsiang, Yen, Hsueh‐Chi S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013793/
https://www.ncbi.nlm.nih.gov/pubmed/33469951
http://dx.doi.org/10.15252/embj.2020105846
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author Yeh, Chi‐Wei
Huang, Wei‐Chieh
Hsu, Pang‐Hung
Yeh, Kun‐Hai
Wang, Li‐Chin
Hsu, Paul Wei‐Che
Lin, Hsiu‐Chuan
Chen, Yi‐Ning
Chen, Shu‐Chuan
Yeang, Chen‐Hsiang
Yen, Hsueh‐Chi S
author_facet Yeh, Chi‐Wei
Huang, Wei‐Chieh
Hsu, Pang‐Hung
Yeh, Kun‐Hai
Wang, Li‐Chin
Hsu, Paul Wei‐Che
Lin, Hsiu‐Chuan
Chen, Yi‐Ning
Chen, Shu‐Chuan
Yeang, Chen‐Hsiang
Yen, Hsueh‐Chi S
author_sort Yeh, Chi‐Wei
collection PubMed
description Protein termini are determinants of protein stability. Proteins bearing degradation signals, or degrons, at their amino‐ or carboxyl‐termini are eliminated by the N‐ or C‐degron pathways, respectively. We aimed to elucidate the function of C‐degron pathways and to unveil how normal proteomes are exempt from C‐degron pathway‐mediated destruction. Our data reveal that C‐degron pathways remove mislocalized cellular proteins and cleavage products of deubiquitinating enzymes. Furthermore, the C‐degron and N‐degron pathways cooperate in protein removal. Proteome analysis revealed a shortfall in normal proteins targeted by C‐degron pathways, but not of defective proteins, suggesting proteolysis‐based immunity as a constraint for protein evolution/selection. Our work highlights the importance of protein termini for protein quality surveillance, and the relationship between the functional proteome and protein degradation pathways.
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spelling pubmed-80137932021-04-02 The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes Yeh, Chi‐Wei Huang, Wei‐Chieh Hsu, Pang‐Hung Yeh, Kun‐Hai Wang, Li‐Chin Hsu, Paul Wei‐Che Lin, Hsiu‐Chuan Chen, Yi‐Ning Chen, Shu‐Chuan Yeang, Chen‐Hsiang Yen, Hsueh‐Chi S EMBO J Articles Protein termini are determinants of protein stability. Proteins bearing degradation signals, or degrons, at their amino‐ or carboxyl‐termini are eliminated by the N‐ or C‐degron pathways, respectively. We aimed to elucidate the function of C‐degron pathways and to unveil how normal proteomes are exempt from C‐degron pathway‐mediated destruction. Our data reveal that C‐degron pathways remove mislocalized cellular proteins and cleavage products of deubiquitinating enzymes. Furthermore, the C‐degron and N‐degron pathways cooperate in protein removal. Proteome analysis revealed a shortfall in normal proteins targeted by C‐degron pathways, but not of defective proteins, suggesting proteolysis‐based immunity as a constraint for protein evolution/selection. Our work highlights the importance of protein termini for protein quality surveillance, and the relationship between the functional proteome and protein degradation pathways. John Wiley and Sons Inc. 2021-01-20 2021-04-01 /pmc/articles/PMC8013793/ /pubmed/33469951 http://dx.doi.org/10.15252/embj.2020105846 Text en © 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Articles
Yeh, Chi‐Wei
Huang, Wei‐Chieh
Hsu, Pang‐Hung
Yeh, Kun‐Hai
Wang, Li‐Chin
Hsu, Paul Wei‐Che
Lin, Hsiu‐Chuan
Chen, Yi‐Ning
Chen, Shu‐Chuan
Yeang, Chen‐Hsiang
Yen, Hsueh‐Chi S
The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
title The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
title_full The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
title_fullStr The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
title_full_unstemmed The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
title_short The C‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
title_sort c‐degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013793/
https://www.ncbi.nlm.nih.gov/pubmed/33469951
http://dx.doi.org/10.15252/embj.2020105846
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