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FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family

The Discoidin, CUB, and LCCL domain-containing protein (DCBLD) family consists of two type-I transmembrane scaffolding receptors, DCBLD1 and DCBLD2, which play important roles in development and cancer. The nonreceptor tyrosine kinases FYN and ABL are known to drive phosphorylation of tyrosine resid...

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Autores principales: Schmoker, Anna M., Weinert, Jaye L., Markwood, Jacob M., Albretsen, Kathryn S., Lunde, Michelle L., Weir, Marion E., Ebert, Alicia M., Hinkle, Karen L., Ballif, Bryan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015000/
https://www.ncbi.nlm.nih.gov/pubmed/32606017
http://dx.doi.org/10.1074/mcp.RA120.002163
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author Schmoker, Anna M.
Weinert, Jaye L.
Markwood, Jacob M.
Albretsen, Kathryn S.
Lunde, Michelle L.
Weir, Marion E.
Ebert, Alicia M.
Hinkle, Karen L.
Ballif, Bryan A.
author_facet Schmoker, Anna M.
Weinert, Jaye L.
Markwood, Jacob M.
Albretsen, Kathryn S.
Lunde, Michelle L.
Weir, Marion E.
Ebert, Alicia M.
Hinkle, Karen L.
Ballif, Bryan A.
author_sort Schmoker, Anna M.
collection PubMed
description The Discoidin, CUB, and LCCL domain-containing protein (DCBLD) family consists of two type-I transmembrane scaffolding receptors, DCBLD1 and DCBLD2, which play important roles in development and cancer. The nonreceptor tyrosine kinases FYN and ABL are known to drive phosphorylation of tyrosine residues in YXXP motifs within the intracellular domains of DCBLD family members, which leads to the recruitment of the Src homology 2 (SH2) domain of the adaptors CT10 regulator of kinase (CRK) and CRK-like (CRKL). We previously characterized the FYN- and ABL-driven phosphorylation of DCBLD family YXXP motifs. However, we have identified additional FYN- and ABL-dependent phosphorylation sites on DCBLD1 and DCBLD2. This suggests that beyond CRK and CRKL, additional DCBLD interactors may be regulated by FYN and ABL activity. Here, we report a quantitative proteomics approach in which we map the FYN- and ABL-regulated interactomes of DCBLD family members. We found FYN and ABL regulated the binding of several signaling molecules to DCBLD1 and DCBLD2, including members of the 14-3-3 family of adaptors. Biochemical investigation of the DCBLD2/14-3-3 interaction revealed ABL-induced binding of 14-3-3 family members directly to DCBLD2.
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spelling pubmed-80150002021-04-12 FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family Schmoker, Anna M. Weinert, Jaye L. Markwood, Jacob M. Albretsen, Kathryn S. Lunde, Michelle L. Weir, Marion E. Ebert, Alicia M. Hinkle, Karen L. Ballif, Bryan A. Mol Cell Proteomics Research The Discoidin, CUB, and LCCL domain-containing protein (DCBLD) family consists of two type-I transmembrane scaffolding receptors, DCBLD1 and DCBLD2, which play important roles in development and cancer. The nonreceptor tyrosine kinases FYN and ABL are known to drive phosphorylation of tyrosine residues in YXXP motifs within the intracellular domains of DCBLD family members, which leads to the recruitment of the Src homology 2 (SH2) domain of the adaptors CT10 regulator of kinase (CRK) and CRK-like (CRKL). We previously characterized the FYN- and ABL-driven phosphorylation of DCBLD family YXXP motifs. However, we have identified additional FYN- and ABL-dependent phosphorylation sites on DCBLD1 and DCBLD2. This suggests that beyond CRK and CRKL, additional DCBLD interactors may be regulated by FYN and ABL activity. Here, we report a quantitative proteomics approach in which we map the FYN- and ABL-regulated interactomes of DCBLD family members. We found FYN and ABL regulated the binding of several signaling molecules to DCBLD1 and DCBLD2, including members of the 14-3-3 family of adaptors. Biochemical investigation of the DCBLD2/14-3-3 interaction revealed ABL-induced binding of 14-3-3 family members directly to DCBLD2. American Society for Biochemistry and Molecular Biology 2020-11-25 /pmc/articles/PMC8015000/ /pubmed/32606017 http://dx.doi.org/10.1074/mcp.RA120.002163 Text en © 2020 © 2020 Schmoker et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research
Schmoker, Anna M.
Weinert, Jaye L.
Markwood, Jacob M.
Albretsen, Kathryn S.
Lunde, Michelle L.
Weir, Marion E.
Ebert, Alicia M.
Hinkle, Karen L.
Ballif, Bryan A.
FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family
title FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family
title_full FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family
title_fullStr FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family
title_full_unstemmed FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family
title_short FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family
title_sort fyn and abl regulate the interaction networks of the dcbld receptor family
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015000/
https://www.ncbi.nlm.nih.gov/pubmed/32606017
http://dx.doi.org/10.1074/mcp.RA120.002163
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