Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues

BACKGROUND: The recent studies highlighted the critical role of exosomes in the regulation of inflammation. Here, we investigated the dynamic biogenesis of the exosomes in the rat model of asthma. RESULTS: Our finding showed an increase in the expression of IL-4 and the suppression of IL-10 in asthm...

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Autores principales: Almohammai, Asheed, Rahbarghazi, Reza, Keyhanmanesh, Rana, Rezaie, Jafar, Ahmadi, Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015058/
https://www.ncbi.nlm.nih.gov/pubmed/33794910
http://dx.doi.org/10.1186/s12950-021-00275-7
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author Almohammai, Asheed
Rahbarghazi, Reza
Keyhanmanesh, Rana
Rezaie, Jafar
Ahmadi, Mahdi
author_facet Almohammai, Asheed
Rahbarghazi, Reza
Keyhanmanesh, Rana
Rezaie, Jafar
Ahmadi, Mahdi
author_sort Almohammai, Asheed
collection PubMed
description BACKGROUND: The recent studies highlighted the critical role of exosomes in the regulation of inflammation. Here, we investigated the dynamic biogenesis of the exosomes in the rat model of asthma. RESULTS: Our finding showed an increase in the expression of IL-4 and the suppression of IL-10 in asthmatic lung tissues compared to the control samples (p < 0.05). Along with the promotion of IL-4, the protein level of TNF-α was induced, showing an active inflammatory status in OVA-sensitized rats. According to our data, the promotion of asthmatic responses increased exosome biogenesis indicated by increased CD63 levels and acetylcholine esterase activity compared to the normal condition (p < 0.05). CONCLUSION: Data suggest that the stimulation of inflammatory response in asthmatic rats could simultaneously increase the paracrine activity of pulmonary cells via the exosome biogenesis. Exosome biogenesis may correlate with the inflammatory response.
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spelling pubmed-80150582021-04-01 Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues Almohammai, Asheed Rahbarghazi, Reza Keyhanmanesh, Rana Rezaie, Jafar Ahmadi, Mahdi J Inflamm (Lond) Research BACKGROUND: The recent studies highlighted the critical role of exosomes in the regulation of inflammation. Here, we investigated the dynamic biogenesis of the exosomes in the rat model of asthma. RESULTS: Our finding showed an increase in the expression of IL-4 and the suppression of IL-10 in asthmatic lung tissues compared to the control samples (p < 0.05). Along with the promotion of IL-4, the protein level of TNF-α was induced, showing an active inflammatory status in OVA-sensitized rats. According to our data, the promotion of asthmatic responses increased exosome biogenesis indicated by increased CD63 levels and acetylcholine esterase activity compared to the normal condition (p < 0.05). CONCLUSION: Data suggest that the stimulation of inflammatory response in asthmatic rats could simultaneously increase the paracrine activity of pulmonary cells via the exosome biogenesis. Exosome biogenesis may correlate with the inflammatory response. BioMed Central 2021-04-01 /pmc/articles/PMC8015058/ /pubmed/33794910 http://dx.doi.org/10.1186/s12950-021-00275-7 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Almohammai, Asheed
Rahbarghazi, Reza
Keyhanmanesh, Rana
Rezaie, Jafar
Ahmadi, Mahdi
Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
title Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
title_full Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
title_fullStr Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
title_full_unstemmed Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
title_short Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
title_sort asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015058/
https://www.ncbi.nlm.nih.gov/pubmed/33794910
http://dx.doi.org/10.1186/s12950-021-00275-7
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