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Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions

[Image: see text] Phenothiazine dyes, methylene blue, new methylene blue, azure A, and azure B, photosensitized the oxidation of nicotinamide adenine dinucleotide (NADH), an important coenzyme in the living cells, through electron transfer. The reduced forms of these phenothiazine dyes, which were p...

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Autores principales: Hirakawa, Kazutaka, Mori, Mizuho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015084/
https://www.ncbi.nlm.nih.gov/pubmed/33817524
http://dx.doi.org/10.1021/acsomega.1c00484
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author Hirakawa, Kazutaka
Mori, Mizuho
author_facet Hirakawa, Kazutaka
Mori, Mizuho
author_sort Hirakawa, Kazutaka
collection PubMed
description [Image: see text] Phenothiazine dyes, methylene blue, new methylene blue, azure A, and azure B, photosensitized the oxidation of nicotinamide adenine dinucleotide (NADH), an important coenzyme in the living cells, through electron transfer. The reduced forms of these phenothiazine dyes, which were produced through electron extraction from NADH, underwent reoxidation to the original cationic forms, leading to the construction of a photoredox cycle. This reoxidation process was the rate-determining step in the photoredox cycle. The electron extraction from NADH using phenothiazine dyes can trigger the chain reaction of the NADH oxidation. Copper ions enhanced the photoredox cycle through reoxidation of the reduced forms of phenothiazine dyes. New methylene blue demonstrated the highest photooxidative activity in this experiment due to the fast reoxidation process. Electron-transfer-mediated oxidation and the role of endogenous metal ions may be important elements in the photosterilization mechanism.
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spelling pubmed-80150842021-04-02 Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions Hirakawa, Kazutaka Mori, Mizuho ACS Omega [Image: see text] Phenothiazine dyes, methylene blue, new methylene blue, azure A, and azure B, photosensitized the oxidation of nicotinamide adenine dinucleotide (NADH), an important coenzyme in the living cells, through electron transfer. The reduced forms of these phenothiazine dyes, which were produced through electron extraction from NADH, underwent reoxidation to the original cationic forms, leading to the construction of a photoredox cycle. This reoxidation process was the rate-determining step in the photoredox cycle. The electron extraction from NADH using phenothiazine dyes can trigger the chain reaction of the NADH oxidation. Copper ions enhanced the photoredox cycle through reoxidation of the reduced forms of phenothiazine dyes. New methylene blue demonstrated the highest photooxidative activity in this experiment due to the fast reoxidation process. Electron-transfer-mediated oxidation and the role of endogenous metal ions may be important elements in the photosterilization mechanism. American Chemical Society 2021-03-17 /pmc/articles/PMC8015084/ /pubmed/33817524 http://dx.doi.org/10.1021/acsomega.1c00484 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Hirakawa, Kazutaka
Mori, Mizuho
Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions
title Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions
title_full Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions
title_fullStr Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions
title_full_unstemmed Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions
title_short Phenothiazine Dyes Induce NADH Photooxidation through Electron Transfer: Kinetics and the Effect of Copper Ions
title_sort phenothiazine dyes induce nadh photooxidation through electron transfer: kinetics and the effect of copper ions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015084/
https://www.ncbi.nlm.nih.gov/pubmed/33817524
http://dx.doi.org/10.1021/acsomega.1c00484
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