Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T

[Image: see text] Thioflavin T fluorescence is a gold standard probe for the detection of amyloid fibrils. Herein, we showed that mature amyloid fibrils incubated with polyphenol epigallocatechin gallate (EGCG) present a fast reduction of the thioflavin T fluorescence, which is not related to remode...

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Autores principales: Kelley, Mirian, Sant’Anna, Ricardo, Fernandes, Luiza, Palhano, Fernando L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015118/
https://www.ncbi.nlm.nih.gov/pubmed/33817533
http://dx.doi.org/10.1021/acsomega.1c00680
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author Kelley, Mirian
Sant’Anna, Ricardo
Fernandes, Luiza
Palhano, Fernando L.
author_facet Kelley, Mirian
Sant’Anna, Ricardo
Fernandes, Luiza
Palhano, Fernando L.
author_sort Kelley, Mirian
collection PubMed
description [Image: see text] Thioflavin T fluorescence is a gold standard probe for the detection of amyloid fibrils. Herein, we showed that mature amyloid fibrils incubated with polyphenol epigallocatechin gallate (EGCG) present a fast reduction of the thioflavin T fluorescence, which is not related to remodeling activity. We propose the use of the pentameric thiophene fluorescence for monitoring the polyphenol remodeling activity.
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spelling pubmed-80151182021-04-02 Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T Kelley, Mirian Sant’Anna, Ricardo Fernandes, Luiza Palhano, Fernando L. ACS Omega [Image: see text] Thioflavin T fluorescence is a gold standard probe for the detection of amyloid fibrils. Herein, we showed that mature amyloid fibrils incubated with polyphenol epigallocatechin gallate (EGCG) present a fast reduction of the thioflavin T fluorescence, which is not related to remodeling activity. We propose the use of the pentameric thiophene fluorescence for monitoring the polyphenol remodeling activity. American Chemical Society 2021-03-16 /pmc/articles/PMC8015118/ /pubmed/33817533 http://dx.doi.org/10.1021/acsomega.1c00680 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Kelley, Mirian
Sant’Anna, Ricardo
Fernandes, Luiza
Palhano, Fernando L.
Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T
title Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T
title_full Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T
title_fullStr Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T
title_full_unstemmed Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T
title_short Pentameric Thiophene as a Probe to Monitor EGCG’s Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T
title_sort pentameric thiophene as a probe to monitor egcg’s remodeling activity of mature amyloid fibrils: overcoming signal artifacts of thioflavin t
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015118/
https://www.ncbi.nlm.nih.gov/pubmed/33817533
http://dx.doi.org/10.1021/acsomega.1c00680
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