Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey

Pratylenchus vulnus (walnut root lesion nematode) is one of the most damaging root lesion nematode species worldwide. In this study, 17 populations of P. vulnus obtained from wheat and barley cultivated fields in 2016 to 2017 in Turkey (Karaman and Konya provinces) were identified using real-time PC...

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Autores principales: Karaca, Mehmet Sait, Yavuzaslanoglu, Elif, Imriz, Gul, Sonmezoglu, Ozlem Ates
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2020
Materias:
Arts & Humanities
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015327/
https://www.ncbi.nlm.nih.gov/pubmed/33829183
http://dx.doi.org/10.21307/jofnem-2020-084
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author Karaca, Mehmet Sait
Yavuzaslanoglu, Elif
Imriz, Gul
Sonmezoglu, Ozlem Ates
author_facet Karaca, Mehmet Sait
Yavuzaslanoglu, Elif
Imriz, Gul
Sonmezoglu, Ozlem Ates
author_sort Karaca, Mehmet Sait
collection PubMed
description Pratylenchus vulnus (walnut root lesion nematode) is one of the most damaging root lesion nematode species worldwide. In this study, 17 populations of P. vulnus obtained from wheat and barley cultivated fields in 2016 to 2017 in Turkey (Karaman and Konya provinces) were identified using real-time PCR and melting curve analysis. Samples provided a single peak at 87.3˚C with real-time PCR. D2 to D3 expansion segments of the 28S rRNA of one population from Cihanbeyli district in Konya province was sequenced and recorded in GenBank (Accession number: MT320536.1). Alignments of the population was identical 98.66% to the populations of P. vulnus available in GenBank (Accs. nos: LT985479.1 and LT965052.1) and 98.65% (Accs. nos: KY424305.1 and KY424304.1).
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spelling pubmed-80153272021-04-06 Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey Karaca, Mehmet Sait Yavuzaslanoglu, Elif Imriz, Gul Sonmezoglu, Ozlem Ates J Nematol Arts & Humanities Pratylenchus vulnus (walnut root lesion nematode) is one of the most damaging root lesion nematode species worldwide. In this study, 17 populations of P. vulnus obtained from wheat and barley cultivated fields in 2016 to 2017 in Turkey (Karaman and Konya provinces) were identified using real-time PCR and melting curve analysis. Samples provided a single peak at 87.3˚C with real-time PCR. D2 to D3 expansion segments of the 28S rRNA of one population from Cihanbeyli district in Konya province was sequenced and recorded in GenBank (Accession number: MT320536.1). Alignments of the population was identical 98.66% to the populations of P. vulnus available in GenBank (Accs. nos: LT985479.1 and LT965052.1) and 98.65% (Accs. nos: KY424305.1 and KY424304.1). Exeley Inc. 2020-08-25 /pmc/articles/PMC8015327/ /pubmed/33829183 http://dx.doi.org/10.21307/jofnem-2020-084 Text en © 2020 Authors https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This is an Open Access article licensed under the Creative Commons CC BY 4.0 license, https://creativecommons.org/licenses/by/4.0/
spellingShingle Arts & Humanities
Karaca, Mehmet Sait
Yavuzaslanoglu, Elif
Imriz, Gul
Sonmezoglu, Ozlem Ates
Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
title Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
title_full Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
title_fullStr Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
title_full_unstemmed Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
title_short Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
title_sort molecular characterization of the pratylenchus vulnus populations on cereals in turkey
topic Arts & Humanities
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015327/
https://www.ncbi.nlm.nih.gov/pubmed/33829183
http://dx.doi.org/10.21307/jofnem-2020-084
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