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Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes

Neuropeptide B (NPB) is reported to regulate energy homeostasis and metabolism via the NPBWR1 and NPBWR2 receptors in various tissues. However, the molecular mechanisms triggered from their interaction are not well investigated in brown adipose tissue. In this study, we specifically analyzed the rol...

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Autores principales: Wojciechowicz, Tatiana, Billert, Maria, Dhandapani, Priyavathi, Szczepankiewicz, Dawid, Wasielewski, Oskar, Strowski, Mathias Z., Nowak, Krzysztof W., Skrzypski, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016125/
https://www.ncbi.nlm.nih.gov/pubmed/33629519
http://dx.doi.org/10.1002/2211-5463.13128
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author Wojciechowicz, Tatiana
Billert, Maria
Dhandapani, Priyavathi
Szczepankiewicz, Dawid
Wasielewski, Oskar
Strowski, Mathias Z.
Nowak, Krzysztof W.
Skrzypski, Marek
author_facet Wojciechowicz, Tatiana
Billert, Maria
Dhandapani, Priyavathi
Szczepankiewicz, Dawid
Wasielewski, Oskar
Strowski, Mathias Z.
Nowak, Krzysztof W.
Skrzypski, Marek
author_sort Wojciechowicz, Tatiana
collection PubMed
description Neuropeptide B (NPB) is reported to regulate energy homeostasis and metabolism via the NPBWR1 and NPBWR2 receptors in various tissues. However, the molecular mechanisms triggered from their interaction are not well investigated in brown adipose tissue. In this study, we specifically analyzed the role of NPB in controlling brown adipogenesis in rat brown preadipocytes. We first detected the expression of NPBWR1 and NPB on mRNA and protein level in brown preadipocytes and observed that NPB increased viability and proliferation of preadipocytes. Moreover, NPB stimulated expression of adipogenic genes (Prdm16, Ucp1) and suppressed the expression of antiadipogenic preadipocyte factor 1 (Pref1) during the differentiation process. Altogether, this led to an increase in intracellular lipid accumulation during preadipocyte differentiation, coupled with an increase in adrenaline‐induced oxygen consumption mediated by NPB. Furthermore, Ucp1 expression stimulated by NPB was attenuated by blockade of p38 kinase. In summary, we conclude that NPB promotes proliferation and differentiation of rat brown preadipocytes via p38‐dependent mechanism and plays an important role in controlling brown adipose tissue formation.
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spelling pubmed-80161252021-04-02 Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes Wojciechowicz, Tatiana Billert, Maria Dhandapani, Priyavathi Szczepankiewicz, Dawid Wasielewski, Oskar Strowski, Mathias Z. Nowak, Krzysztof W. Skrzypski, Marek FEBS Open Bio Research Articles Neuropeptide B (NPB) is reported to regulate energy homeostasis and metabolism via the NPBWR1 and NPBWR2 receptors in various tissues. However, the molecular mechanisms triggered from their interaction are not well investigated in brown adipose tissue. In this study, we specifically analyzed the role of NPB in controlling brown adipogenesis in rat brown preadipocytes. We first detected the expression of NPBWR1 and NPB on mRNA and protein level in brown preadipocytes and observed that NPB increased viability and proliferation of preadipocytes. Moreover, NPB stimulated expression of adipogenic genes (Prdm16, Ucp1) and suppressed the expression of antiadipogenic preadipocyte factor 1 (Pref1) during the differentiation process. Altogether, this led to an increase in intracellular lipid accumulation during preadipocyte differentiation, coupled with an increase in adrenaline‐induced oxygen consumption mediated by NPB. Furthermore, Ucp1 expression stimulated by NPB was attenuated by blockade of p38 kinase. In summary, we conclude that NPB promotes proliferation and differentiation of rat brown preadipocytes via p38‐dependent mechanism and plays an important role in controlling brown adipose tissue formation. John Wiley and Sons Inc. 2021-03-12 /pmc/articles/PMC8016125/ /pubmed/33629519 http://dx.doi.org/10.1002/2211-5463.13128 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Wojciechowicz, Tatiana
Billert, Maria
Dhandapani, Priyavathi
Szczepankiewicz, Dawid
Wasielewski, Oskar
Strowski, Mathias Z.
Nowak, Krzysztof W.
Skrzypski, Marek
Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
title Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
title_full Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
title_fullStr Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
title_full_unstemmed Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
title_short Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
title_sort neuropeptide b promotes proliferation and differentiation of rat brown primary preadipocytes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016125/
https://www.ncbi.nlm.nih.gov/pubmed/33629519
http://dx.doi.org/10.1002/2211-5463.13128
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