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C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer

C/EBPβ is a member of the CCAAT/enhancer‐binding protein (C/EBP) family, which consists of a number of b‐ZIP transcription factors. Although C/EBPβ has been implicated in the development of certain cancers, including breast cancer, it remains unknown whether dysregulation of C/EBPβ in breast cancer...

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Detalles Bibliográficos
Autores principales: Wang, Shu, Xia, Dian, Wang, Xianzhi, Cao, Haowei, Wu, Chaoshen, Sun, Zhaoran, Zhang, Daoyong, Liu, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016132/
https://www.ncbi.nlm.nih.gov/pubmed/33660927
http://dx.doi.org/10.1002/2211-5463.13138
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author Wang, Shu
Xia, Dian
Wang, Xianzhi
Cao, Haowei
Wu, Chaoshen
Sun, Zhaoran
Zhang, Daoyong
Liu, Hao
author_facet Wang, Shu
Xia, Dian
Wang, Xianzhi
Cao, Haowei
Wu, Chaoshen
Sun, Zhaoran
Zhang, Daoyong
Liu, Hao
author_sort Wang, Shu
collection PubMed
description C/EBPβ is a member of the CCAAT/enhancer‐binding protein (C/EBP) family, which consists of a number of b‐ZIP transcription factors. Although C/EBPβ has been implicated in the development of certain cancers, including breast cancer, it remains unknown whether dysregulation of C/EBPβ in breast cancer is subtype‐specific. Moreover, the underlying mechanisms by which C/EBPβ regulates breast cancer carcinogenesis are not fully understood. Here, we present evidence that C/EBPβ is specifically overexpressed in human TNBC samples, but not in non‐TNBC samples. C/EBPβ depletion dramatically suppressed TNBC cell growth, migration, invasion, and colony formation ability. A subsequent mechanistic study revealed that the JAK/STAT signaling pathway was upregulated in C/EBPβ_high TNBC samples compared with C/EBPβ_low TNBC samples. C/EBPβ ChIP‐seq and qPCR were performed to demonstrate that C/EBPβ directly binds to and regulates JAK/STAT signaling pathway genes in TNBC. Taken together, our data indicate the oncogenic role of C/EBPβ in human TNBC and reveal a novel mechanism by which C/EBPβ promotes TNBC carcinogenesis.
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spelling pubmed-80161322021-04-02 C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer Wang, Shu Xia, Dian Wang, Xianzhi Cao, Haowei Wu, Chaoshen Sun, Zhaoran Zhang, Daoyong Liu, Hao FEBS Open Bio Research Articles C/EBPβ is a member of the CCAAT/enhancer‐binding protein (C/EBP) family, which consists of a number of b‐ZIP transcription factors. Although C/EBPβ has been implicated in the development of certain cancers, including breast cancer, it remains unknown whether dysregulation of C/EBPβ in breast cancer is subtype‐specific. Moreover, the underlying mechanisms by which C/EBPβ regulates breast cancer carcinogenesis are not fully understood. Here, we present evidence that C/EBPβ is specifically overexpressed in human TNBC samples, but not in non‐TNBC samples. C/EBPβ depletion dramatically suppressed TNBC cell growth, migration, invasion, and colony formation ability. A subsequent mechanistic study revealed that the JAK/STAT signaling pathway was upregulated in C/EBPβ_high TNBC samples compared with C/EBPβ_low TNBC samples. C/EBPβ ChIP‐seq and qPCR were performed to demonstrate that C/EBPβ directly binds to and regulates JAK/STAT signaling pathway genes in TNBC. Taken together, our data indicate the oncogenic role of C/EBPβ in human TNBC and reveal a novel mechanism by which C/EBPβ promotes TNBC carcinogenesis. John Wiley and Sons Inc. 2021-03-17 /pmc/articles/PMC8016132/ /pubmed/33660927 http://dx.doi.org/10.1002/2211-5463.13138 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Wang, Shu
Xia, Dian
Wang, Xianzhi
Cao, Haowei
Wu, Chaoshen
Sun, Zhaoran
Zhang, Daoyong
Liu, Hao
C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer
title C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer
title_full C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer
title_fullStr C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer
title_full_unstemmed C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer
title_short C/EBPβ regulates the JAK/STAT signaling pathway in triple‐negative breast cancer
title_sort c/ebpβ regulates the jak/stat signaling pathway in triple‐negative breast cancer
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016132/
https://www.ncbi.nlm.nih.gov/pubmed/33660927
http://dx.doi.org/10.1002/2211-5463.13138
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