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Observation of arenavirus nucleoprotein heptamer assembly

Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single‐stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associa...

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Autores principales: Papageorgiou, Nicolas, Vaitsopoulou, Afroditi, Diop, Awa, Nguyen, Thi Hong Van, Canard, Bruno, Alvarez, Karine, Ferron, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016135/
https://www.ncbi.nlm.nih.gov/pubmed/33534950
http://dx.doi.org/10.1002/2211-5463.13106
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author Papageorgiou, Nicolas
Vaitsopoulou, Afroditi
Diop, Awa
Nguyen, Thi Hong Van
Canard, Bruno
Alvarez, Karine
Ferron, François
author_facet Papageorgiou, Nicolas
Vaitsopoulou, Afroditi
Diop, Awa
Nguyen, Thi Hong Van
Canard, Bruno
Alvarez, Karine
Ferron, François
author_sort Papageorgiou, Nicolas
collection PubMed
description Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single‐stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self‐assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full‐length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.
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spelling pubmed-80161352021-04-02 Observation of arenavirus nucleoprotein heptamer assembly Papageorgiou, Nicolas Vaitsopoulou, Afroditi Diop, Awa Nguyen, Thi Hong Van Canard, Bruno Alvarez, Karine Ferron, François FEBS Open Bio Research Articles Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single‐stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self‐assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full‐length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations. John Wiley and Sons Inc. 2021-02-25 /pmc/articles/PMC8016135/ /pubmed/33534950 http://dx.doi.org/10.1002/2211-5463.13106 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Papageorgiou, Nicolas
Vaitsopoulou, Afroditi
Diop, Awa
Nguyen, Thi Hong Van
Canard, Bruno
Alvarez, Karine
Ferron, François
Observation of arenavirus nucleoprotein heptamer assembly
title Observation of arenavirus nucleoprotein heptamer assembly
title_full Observation of arenavirus nucleoprotein heptamer assembly
title_fullStr Observation of arenavirus nucleoprotein heptamer assembly
title_full_unstemmed Observation of arenavirus nucleoprotein heptamer assembly
title_short Observation of arenavirus nucleoprotein heptamer assembly
title_sort observation of arenavirus nucleoprotein heptamer assembly
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016135/
https://www.ncbi.nlm.nih.gov/pubmed/33534950
http://dx.doi.org/10.1002/2211-5463.13106
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