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Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research

Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to ad...

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Autores principales: Richter, Łukasz, Księżarczyk, Karolina, Paszkowska, Karolina, Janczuk-Richter, Marta, Niedziółka-Jönsson, Joanna, Gapiński, Jacek, Łoś, Marcin, Hołyst, Robert, Paczesny, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016829/
https://www.ncbi.nlm.nih.gov/pubmed/33795704
http://dx.doi.org/10.1038/s41598-021-86571-x
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author Richter, Łukasz
Księżarczyk, Karolina
Paszkowska, Karolina
Janczuk-Richter, Marta
Niedziółka-Jönsson, Joanna
Gapiński, Jacek
Łoś, Marcin
Hołyst, Robert
Paczesny, Jan
author_facet Richter, Łukasz
Księżarczyk, Karolina
Paszkowska, Karolina
Janczuk-Richter, Marta
Niedziółka-Jönsson, Joanna
Gapiński, Jacek
Łoś, Marcin
Hołyst, Robert
Paczesny, Jan
author_sort Richter, Łukasz
collection PubMed
description Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 10(8) PFU/ml to around 10(10) PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.
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spelling pubmed-80168292021-04-05 Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research Richter, Łukasz Księżarczyk, Karolina Paszkowska, Karolina Janczuk-Richter, Marta Niedziółka-Jönsson, Joanna Gapiński, Jacek Łoś, Marcin Hołyst, Robert Paczesny, Jan Sci Rep Article Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 10(8) PFU/ml to around 10(10) PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology. Nature Publishing Group UK 2021-04-01 /pmc/articles/PMC8016829/ /pubmed/33795704 http://dx.doi.org/10.1038/s41598-021-86571-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Richter, Łukasz
Księżarczyk, Karolina
Paszkowska, Karolina
Janczuk-Richter, Marta
Niedziółka-Jönsson, Joanna
Gapiński, Jacek
Łoś, Marcin
Hołyst, Robert
Paczesny, Jan
Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_full Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_fullStr Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_full_unstemmed Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_short Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_sort adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016829/
https://www.ncbi.nlm.nih.gov/pubmed/33795704
http://dx.doi.org/10.1038/s41598-021-86571-x
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