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The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of und...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016914/ https://www.ncbi.nlm.nih.gov/pubmed/33795805 http://dx.doi.org/10.1038/s41598-021-86975-9 |
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author | Nicol, Lauren Croce, Roberta |
author_facet | Nicol, Lauren Croce, Roberta |
author_sort | Nicol, Lauren |
collection | PubMed |
description | Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII. |
format | Online Article Text |
id | pubmed-8016914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80169142021-04-05 The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII Nicol, Lauren Croce, Roberta Sci Rep Article Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII. Nature Publishing Group UK 2021-04-01 /pmc/articles/PMC8016914/ /pubmed/33795805 http://dx.doi.org/10.1038/s41598-021-86975-9 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nicol, Lauren Croce, Roberta The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII |
title | The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII |
title_full | The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII |
title_fullStr | The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII |
title_full_unstemmed | The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII |
title_short | The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII |
title_sort | psbs protein and low ph are necessary and sufficient to induce quenching in the light-harvesting complex of plants lhcii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016914/ https://www.ncbi.nlm.nih.gov/pubmed/33795805 http://dx.doi.org/10.1038/s41598-021-86975-9 |
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