Cargando…

Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development

Circular RNAs (circRNAs) have been shown to function in the reproductive systems including testis. However, their expression, as well as function in testicular development of sheep remain undefined. Herein, we performed RNA sequencing to reveal circRNA temporal expression patterns in testes of Tibet...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Taotao, Luo, Ruirui, Wang, Xia, Wang, Huihui, Zhao, Xingxu, Guo, Yunxia, Jiang, Haitao, Ma, Youji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017185/
https://www.ncbi.nlm.nih.gov/pubmed/33816472
http://dx.doi.org/10.3389/fcell.2021.627439
_version_ 1783674009591742464
author Li, Taotao
Luo, Ruirui
Wang, Xia
Wang, Huihui
Zhao, Xingxu
Guo, Yunxia
Jiang, Haitao
Ma, Youji
author_facet Li, Taotao
Luo, Ruirui
Wang, Xia
Wang, Huihui
Zhao, Xingxu
Guo, Yunxia
Jiang, Haitao
Ma, Youji
author_sort Li, Taotao
collection PubMed
description Circular RNAs (circRNAs) have been shown to function in the reproductive systems including testis. However, their expression, as well as function in testicular development of sheep remain undefined. Herein, we performed RNA sequencing to reveal circRNA temporal expression patterns in testes of Tibetan sheep from different stages of maturation (3M, 3-month-old; 1Y, 1-year-old; 3Y, 3-year-old). A total of 3,982, 414, and 4,060 differentially expressed (DE) circRNAs were uncovered from 3M vs 1Y, 1Y vs 3Y, and 3M vs 3Y, respectively. Functional enrichment assessment indicated that the source genes of DE circRNAs were primarily engaged in spermatogenesis and testicular immune privilege including blood–testis barrier (BTB). We subsequently constructed the core circRNA–miRNA–mRNA interaction network for genes related to testicular function, such as spermatogenesis, germ cell development, BTB, and cell cycle/meiosis. Furthermore, we validated the target associations between either circ_024949, circ_026259 or IGF1, and oar-miR-29b in this network, and revealed their similar expression signatures in developmental testes that they were extensively expressed in germ cells, Leydig cells, and Sertoli cells, thus suggesting their broad functions in the functional maintenance of Leydig cells and Sertoli cells, as well as the development and maturation of male germ cells. Meanwhile, circ_026259 was shown to promote IGF1 expression through inhibition of oar-miR-29b in sheep Sertoli cells. This work gives the first global view for the expression and regulation of circRNAs in sheep testis, which will be helpful for providing new insights into the molecular mechanism of ovine testis function.
format Online
Article
Text
id pubmed-8017185
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80171852021-04-03 Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development Li, Taotao Luo, Ruirui Wang, Xia Wang, Huihui Zhao, Xingxu Guo, Yunxia Jiang, Haitao Ma, Youji Front Cell Dev Biol Cell and Developmental Biology Circular RNAs (circRNAs) have been shown to function in the reproductive systems including testis. However, their expression, as well as function in testicular development of sheep remain undefined. Herein, we performed RNA sequencing to reveal circRNA temporal expression patterns in testes of Tibetan sheep from different stages of maturation (3M, 3-month-old; 1Y, 1-year-old; 3Y, 3-year-old). A total of 3,982, 414, and 4,060 differentially expressed (DE) circRNAs were uncovered from 3M vs 1Y, 1Y vs 3Y, and 3M vs 3Y, respectively. Functional enrichment assessment indicated that the source genes of DE circRNAs were primarily engaged in spermatogenesis and testicular immune privilege including blood–testis barrier (BTB). We subsequently constructed the core circRNA–miRNA–mRNA interaction network for genes related to testicular function, such as spermatogenesis, germ cell development, BTB, and cell cycle/meiosis. Furthermore, we validated the target associations between either circ_024949, circ_026259 or IGF1, and oar-miR-29b in this network, and revealed their similar expression signatures in developmental testes that they were extensively expressed in germ cells, Leydig cells, and Sertoli cells, thus suggesting their broad functions in the functional maintenance of Leydig cells and Sertoli cells, as well as the development and maturation of male germ cells. Meanwhile, circ_026259 was shown to promote IGF1 expression through inhibition of oar-miR-29b in sheep Sertoli cells. This work gives the first global view for the expression and regulation of circRNAs in sheep testis, which will be helpful for providing new insights into the molecular mechanism of ovine testis function. Frontiers Media S.A. 2021-03-19 /pmc/articles/PMC8017185/ /pubmed/33816472 http://dx.doi.org/10.3389/fcell.2021.627439 Text en Copyright © 2021 Li, Luo, Wang, Wang, Zhao, Guo, Jiang and Ma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Li, Taotao
Luo, Ruirui
Wang, Xia
Wang, Huihui
Zhao, Xingxu
Guo, Yunxia
Jiang, Haitao
Ma, Youji
Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development
title Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development
title_full Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development
title_fullStr Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development
title_full_unstemmed Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development
title_short Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development
title_sort unraveling stage-dependent expression patterns of circular rnas and their related cerna modulation in ovine postnatal testis development
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017185/
https://www.ncbi.nlm.nih.gov/pubmed/33816472
http://dx.doi.org/10.3389/fcell.2021.627439
work_keys_str_mv AT litaotao unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT luoruirui unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT wangxia unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT wanghuihui unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT zhaoxingxu unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT guoyunxia unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT jianghaitao unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment
AT mayouji unravelingstagedependentexpressionpatternsofcircularrnasandtheirrelatedcernamodulationinovinepostnataltestisdevelopment