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Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro

Aptazyme and CRISPR/Cas gene editing system were widely used for regulating gene expression in various diseases, including cancer. This work aimed to reconstruct CRISPR/Cas13d tool for sensing hTERT exclusively based on the new device OFF-switch hTERT aptazyme that was inserted into the 3’ UTR of th...

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Autores principales: Zhuang, Chengle, Zhuang, Changshui, Zhou, Qun, Huang, Xueting, Gui, Yaoting, Lai, Yongqing, Yang, Shangqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017217/
https://www.ncbi.nlm.nih.gov/pubmed/33816560
http://dx.doi.org/10.3389/fmolb.2021.646412
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author Zhuang, Chengle
Zhuang, Changshui
Zhou, Qun
Huang, Xueting
Gui, Yaoting
Lai, Yongqing
Yang, Shangqi
author_facet Zhuang, Chengle
Zhuang, Changshui
Zhou, Qun
Huang, Xueting
Gui, Yaoting
Lai, Yongqing
Yang, Shangqi
author_sort Zhuang, Chengle
collection PubMed
description Aptazyme and CRISPR/Cas gene editing system were widely used for regulating gene expression in various diseases, including cancer. This work aimed to reconstruct CRISPR/Cas13d tool for sensing hTERT exclusively based on the new device OFF-switch hTERT aptazyme that was inserted into the 3’ UTR of the Cas13d. In bladder cancer cells, hTERT ligand bound to aptamer in OFF-switch hTERT aptazyme to inhibit the degradation of Cas13d. Results showed that engineered CRISPR/Cas13d sensing hTERT suppressed cell proliferation, migration, invasion and induced cell apoptosis in bladder cancer 5637 and T24 cells without affecting normal HFF cells. In short, we constructed engineered CRISPR/Cas13d sensing hTERT selectively inhibited the progression of bladder cancer cells significantly. It may serve as a promising specifically effective therapy for bladder cancer cells.
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spelling pubmed-80172172021-04-03 Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro Zhuang, Chengle Zhuang, Changshui Zhou, Qun Huang, Xueting Gui, Yaoting Lai, Yongqing Yang, Shangqi Front Mol Biosci Molecular Biosciences Aptazyme and CRISPR/Cas gene editing system were widely used for regulating gene expression in various diseases, including cancer. This work aimed to reconstruct CRISPR/Cas13d tool for sensing hTERT exclusively based on the new device OFF-switch hTERT aptazyme that was inserted into the 3’ UTR of the Cas13d. In bladder cancer cells, hTERT ligand bound to aptamer in OFF-switch hTERT aptazyme to inhibit the degradation of Cas13d. Results showed that engineered CRISPR/Cas13d sensing hTERT suppressed cell proliferation, migration, invasion and induced cell apoptosis in bladder cancer 5637 and T24 cells without affecting normal HFF cells. In short, we constructed engineered CRISPR/Cas13d sensing hTERT selectively inhibited the progression of bladder cancer cells significantly. It may serve as a promising specifically effective therapy for bladder cancer cells. Frontiers Media S.A. 2021-03-19 /pmc/articles/PMC8017217/ /pubmed/33816560 http://dx.doi.org/10.3389/fmolb.2021.646412 Text en Copyright © 2021 Zhuang, Zhuang, Zhou, Huang, Gui, Lai and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Zhuang, Chengle
Zhuang, Changshui
Zhou, Qun
Huang, Xueting
Gui, Yaoting
Lai, Yongqing
Yang, Shangqi
Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
title Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
title_full Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
title_fullStr Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
title_full_unstemmed Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
title_short Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
title_sort engineered crispr/cas13d sensing htert selectively inhibits the progression of bladder cancer in vitro
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017217/
https://www.ncbi.nlm.nih.gov/pubmed/33816560
http://dx.doi.org/10.3389/fmolb.2021.646412
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