Cargando…

Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes

We constructed a prognostic risk model for colon adenocarcinoma (COAD) using microRNAs (miRNAs) as biomarkers. Clinical data of patients with COADs and miRNA-seq data were from TCGA, and the differential expression of miRNAs (carcinoma vs. para-carcinoma tissues) was assessed using R software. COAD...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Guoliang, Zhang, GuoJun, Zhao, Yan, Zheng, Zhichao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017316/
https://www.ncbi.nlm.nih.gov/pubmed/33816220
http://dx.doi.org/10.3389/fonc.2021.560136
_version_ 1783674040134664192
author Zheng, Guoliang
Zhang, GuoJun
Zhao, Yan
Zheng, Zhichao
author_facet Zheng, Guoliang
Zhang, GuoJun
Zhao, Yan
Zheng, Zhichao
author_sort Zheng, Guoliang
collection PubMed
description We constructed a prognostic risk model for colon adenocarcinoma (COAD) using microRNAs (miRNAs) as biomarkers. Clinical data of patients with COADs and miRNA-seq data were from TCGA, and the differential expression of miRNAs (carcinoma vs. para-carcinoma tissues) was assessed using R software. COAD data were randomly divided into Training and Testing Sets. A linear prognostic risk model was constructed using Cox regression analysis based on the Training Set. Patients were classified as high-risk or low-risk according to the score of the prognostic model. Survival analysis and receiver operating characteristic (ROC) curves were used to evaluate model performance. The gene targets in the prognostic model were identified and their biological functions were analyzed. Analysis of COAD and normal cell lines using qPCR was used to verify the model. There were 134 up-regulated and 140 down-regulated miRNAs. We used the Training Set to develop a prognostic model based on the expression of seven miRNAs. ROC analysis indicated this model had acceptable prediction accuracy (area under the curve=0.784). Kaplan-Meier survival analysis showed that overall survival was worse in the high-risk group. Cox regression analysis showed that the 7-miRNA Risk Score was an independent prognostic factor. The 2,863 predicted target genes were mainly enriched in the MAPK, PI3K-AKT, proteoglycans in cancer, and mTOR signaling pathways. For unknown reasons, expression of these miRNAs in cancerous and normal cells differed somewhat from model predictions. Regardless, the 7-miRNA Risk Score can be used to predict COAD prognosis and may help to guide clinical treatment.
format Online
Article
Text
id pubmed-8017316
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80173162021-04-03 Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes Zheng, Guoliang Zhang, GuoJun Zhao, Yan Zheng, Zhichao Front Oncol Oncology We constructed a prognostic risk model for colon adenocarcinoma (COAD) using microRNAs (miRNAs) as biomarkers. Clinical data of patients with COADs and miRNA-seq data were from TCGA, and the differential expression of miRNAs (carcinoma vs. para-carcinoma tissues) was assessed using R software. COAD data were randomly divided into Training and Testing Sets. A linear prognostic risk model was constructed using Cox regression analysis based on the Training Set. Patients were classified as high-risk or low-risk according to the score of the prognostic model. Survival analysis and receiver operating characteristic (ROC) curves were used to evaluate model performance. The gene targets in the prognostic model were identified and their biological functions were analyzed. Analysis of COAD and normal cell lines using qPCR was used to verify the model. There were 134 up-regulated and 140 down-regulated miRNAs. We used the Training Set to develop a prognostic model based on the expression of seven miRNAs. ROC analysis indicated this model had acceptable prediction accuracy (area under the curve=0.784). Kaplan-Meier survival analysis showed that overall survival was worse in the high-risk group. Cox regression analysis showed that the 7-miRNA Risk Score was an independent prognostic factor. The 2,863 predicted target genes were mainly enriched in the MAPK, PI3K-AKT, proteoglycans in cancer, and mTOR signaling pathways. For unknown reasons, expression of these miRNAs in cancerous and normal cells differed somewhat from model predictions. Regardless, the 7-miRNA Risk Score can be used to predict COAD prognosis and may help to guide clinical treatment. Frontiers Media S.A. 2021-03-19 /pmc/articles/PMC8017316/ /pubmed/33816220 http://dx.doi.org/10.3389/fonc.2021.560136 Text en Copyright © 2021 Zheng, Zhang, Zhao and Zheng http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zheng, Guoliang
Zhang, GuoJun
Zhao, Yan
Zheng, Zhichao
Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes
title Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes
title_full Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes
title_fullStr Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes
title_full_unstemmed Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes
title_short Screening of miRNAs as Prognostic Biomarkers for Colon Adenocarcinoma and Biological Function Analysis of Their Target Genes
title_sort screening of mirnas as prognostic biomarkers for colon adenocarcinoma and biological function analysis of their target genes
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017316/
https://www.ncbi.nlm.nih.gov/pubmed/33816220
http://dx.doi.org/10.3389/fonc.2021.560136
work_keys_str_mv AT zhengguoliang screeningofmirnasasprognosticbiomarkersforcolonadenocarcinomaandbiologicalfunctionanalysisoftheirtargetgenes
AT zhangguojun screeningofmirnasasprognosticbiomarkersforcolonadenocarcinomaandbiologicalfunctionanalysisoftheirtargetgenes
AT zhaoyan screeningofmirnasasprognosticbiomarkersforcolonadenocarcinomaandbiologicalfunctionanalysisoftheirtargetgenes
AT zhengzhichao screeningofmirnasasprognosticbiomarkersforcolonadenocarcinomaandbiologicalfunctionanalysisoftheirtargetgenes