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Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl

Human adipose stem cells (hASCs) were introduced as appropriate candidate due to advantages like ease of isolation, in vitro expansion and lack of immune response. Deprenyl (Dep) was used to induce bone marrow stem cells into neuron-like cells. We investigated the Dep effect on neurotrophin genes ex...

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Autores principales: Amiri, Arezoo, Kashani, Maryam Haji Ghasem, Ghorbanian, Mohammad Taghi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Anatomists 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017458/
https://www.ncbi.nlm.nih.gov/pubmed/33526752
http://dx.doi.org/10.5115/acb.19.229
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author Amiri, Arezoo
Kashani, Maryam Haji Ghasem
Ghorbanian, Mohammad Taghi
author_facet Amiri, Arezoo
Kashani, Maryam Haji Ghasem
Ghorbanian, Mohammad Taghi
author_sort Amiri, Arezoo
collection PubMed
description Human adipose stem cells (hASCs) were introduced as appropriate candidate due to advantages like ease of isolation, in vitro expansion and lack of immune response. Deprenyl (Dep) was used to induce bone marrow stem cells into neuron-like cells. We investigated the Dep effect on neurotrophin genes expression in hASCs and their differentiation into neuron-like cells. The cells were isolated from small pieces of abdominal adipose tissue and subjected to flow cytometry to confirm purification. The osteogenic and adipogenic differentiation were identified. The proliferation rate and neurotrophin genes expression of treated cells were evaluated by MTT, TH immunostaining and RT-PCR. hASCs had positive response to CD44, CD73, CD90, CD105 markers and negative response to CD34 and CD45 markers and differentiated into adipocytes and osteocytes. Exposure to 10–7 M of Dep for 24 hours caused a significant increase of viable cells and BDNF, NTF-3 genes expression as compared to cultured cells in serum free medium and had no effect on the expression of NGF and GDNF genes. Based on our results, Dep is able to induce BDNF, NTF-3 and NTF-4 genes expression and neroun-like morphology in hASCs.
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spelling pubmed-80174582021-04-08 Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl Amiri, Arezoo Kashani, Maryam Haji Ghasem Ghorbanian, Mohammad Taghi Anat Cell Biol Original Article Human adipose stem cells (hASCs) were introduced as appropriate candidate due to advantages like ease of isolation, in vitro expansion and lack of immune response. Deprenyl (Dep) was used to induce bone marrow stem cells into neuron-like cells. We investigated the Dep effect on neurotrophin genes expression in hASCs and their differentiation into neuron-like cells. The cells were isolated from small pieces of abdominal adipose tissue and subjected to flow cytometry to confirm purification. The osteogenic and adipogenic differentiation were identified. The proliferation rate and neurotrophin genes expression of treated cells were evaluated by MTT, TH immunostaining and RT-PCR. hASCs had positive response to CD44, CD73, CD90, CD105 markers and negative response to CD34 and CD45 markers and differentiated into adipocytes and osteocytes. Exposure to 10–7 M of Dep for 24 hours caused a significant increase of viable cells and BDNF, NTF-3 genes expression as compared to cultured cells in serum free medium and had no effect on the expression of NGF and GDNF genes. Based on our results, Dep is able to induce BDNF, NTF-3 and NTF-4 genes expression and neroun-like morphology in hASCs. Korean Association of Anatomists 2021-03-31 2021-03-31 /pmc/articles/PMC8017458/ /pubmed/33526752 http://dx.doi.org/10.5115/acb.19.229 Text en Copyright © 2021. Anatomy & Cell Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Amiri, Arezoo
Kashani, Maryam Haji Ghasem
Ghorbanian, Mohammad Taghi
Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
title Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
title_full Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
title_fullStr Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
title_full_unstemmed Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
title_short Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
title_sort expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017458/
https://www.ncbi.nlm.nih.gov/pubmed/33526752
http://dx.doi.org/10.5115/acb.19.229
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