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Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning
BACKGROUND: The possible use of oxytocin in saliva as an indicator of positive emotions in bovine species has been poorly investigated. In the present study, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017845/ https://www.ncbi.nlm.nih.gov/pubmed/33794896 http://dx.doi.org/10.1186/s12917-021-02838-5 |
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author | López-Arjona, Marina Mainau, Eva Navarro, Elena Contreras-Aguilar, María Dolores Escribano, Damián Mateo, Sandra V. Manteca, Xavier Cerón, José Joaquín Martínez-Subiela, Silvia |
author_facet | López-Arjona, Marina Mainau, Eva Navarro, Elena Contreras-Aguilar, María Dolores Escribano, Damián Mateo, Sandra V. Manteca, Xavier Cerón, José Joaquín Martínez-Subiela, Silvia |
author_sort | López-Arjona, Marina |
collection | PubMed |
description | BACKGROUND: The possible use of oxytocin in saliva as an indicator of positive emotions in bovine species has been poorly investigated. In the present study, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva were developed and validated. Also, the changes in oxytocin in saliva were explored in two different situations. One was around parturition, and for this purpose, saliva samples from 13 cows were collected at three different times: 7 days before the parturition, the day of parturition and 7 days after the parturition. The second situation was weaning and grouping of calves, and for this purpose, saliva from 25 calves was collected at three different times: 1 day before weaning, 2 days after weaning or milk withdrawal and 4 days after grouping calves. RESULTS: In cows, oxytocin concentrations showed an increase on the day of parturition with both assays, while in calves, oxytocin concentrations showed a decrease 4 days after the grouping. CONCLUSIONS: The assays validated in this report could be used for the measurement of oxytocin in bovine saliva and detect changes in this analyte that can occur in different physiological or productive situations such as parturition and weaning. |
format | Online Article Text |
id | pubmed-8017845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80178452021-04-05 Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning López-Arjona, Marina Mainau, Eva Navarro, Elena Contreras-Aguilar, María Dolores Escribano, Damián Mateo, Sandra V. Manteca, Xavier Cerón, José Joaquín Martínez-Subiela, Silvia BMC Vet Res Research Article BACKGROUND: The possible use of oxytocin in saliva as an indicator of positive emotions in bovine species has been poorly investigated. In the present study, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva were developed and validated. Also, the changes in oxytocin in saliva were explored in two different situations. One was around parturition, and for this purpose, saliva samples from 13 cows were collected at three different times: 7 days before the parturition, the day of parturition and 7 days after the parturition. The second situation was weaning and grouping of calves, and for this purpose, saliva from 25 calves was collected at three different times: 1 day before weaning, 2 days after weaning or milk withdrawal and 4 days after grouping calves. RESULTS: In cows, oxytocin concentrations showed an increase on the day of parturition with both assays, while in calves, oxytocin concentrations showed a decrease 4 days after the grouping. CONCLUSIONS: The assays validated in this report could be used for the measurement of oxytocin in bovine saliva and detect changes in this analyte that can occur in different physiological or productive situations such as parturition and weaning. BioMed Central 2021-04-01 /pmc/articles/PMC8017845/ /pubmed/33794896 http://dx.doi.org/10.1186/s12917-021-02838-5 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article López-Arjona, Marina Mainau, Eva Navarro, Elena Contreras-Aguilar, María Dolores Escribano, Damián Mateo, Sandra V. Manteca, Xavier Cerón, José Joaquín Martínez-Subiela, Silvia Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
title | Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
title_full | Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
title_fullStr | Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
title_full_unstemmed | Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
title_short | Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
title_sort | oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017845/ https://www.ncbi.nlm.nih.gov/pubmed/33794896 http://dx.doi.org/10.1186/s12917-021-02838-5 |
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