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Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea

SAFB1 is a DNA and RNA binding protein that is highly expressed in the cerebellum and hippocampus and is involved in the processing of coding and non‐coding RNAs, splicing and dendritic function. We analyzed SAFB1 expression in the post‐mortem brain tissue of spinocerebellar ataxia (SCA), Huntington...

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Autores principales: Buckner, Nicola, Kemp, Kevin C., Scott, Helen L., Shi, Gongyu, Rivers, Caroline, Gialeli, Andriana, Wong, Liang-Fong, Cordero-LLana, Oscar, Allen, Nicholas, Wilkins, Alastair, Uney, James B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018166/
https://www.ncbi.nlm.nih.gov/pubmed/32580238
http://dx.doi.org/10.1111/bpa.12872
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author Buckner, Nicola
Kemp, Kevin C.
Scott, Helen L.
Shi, Gongyu
Rivers, Caroline
Gialeli, Andriana
Wong, Liang-Fong
Cordero-LLana, Oscar
Allen, Nicholas
Wilkins, Alastair
Uney, James B.
author_facet Buckner, Nicola
Kemp, Kevin C.
Scott, Helen L.
Shi, Gongyu
Rivers, Caroline
Gialeli, Andriana
Wong, Liang-Fong
Cordero-LLana, Oscar
Allen, Nicholas
Wilkins, Alastair
Uney, James B.
author_sort Buckner, Nicola
collection PubMed
description SAFB1 is a DNA and RNA binding protein that is highly expressed in the cerebellum and hippocampus and is involved in the processing of coding and non‐coding RNAs, splicing and dendritic function. We analyzed SAFB1 expression in the post‐mortem brain tissue of spinocerebellar ataxia (SCA), Huntington’s disease (HD), Multiple sclerosis (MS), Parkinson’s disease patients and controls. In SCA cases, the expression of SAFB1 in the nucleus was increased and there was abnormal and extensive expression in the cytoplasm where it co‐localized with the markers of Purkinje cell injury. Significantly, no SAFB1 expression was found in the cerebellar neurons of the dentate nucleus in control or MS patients; however, in SCA patients, SAFB1 expression was increased significantly in both the nucleus and cytoplasm of dentate neurons. In HD, we found that SAFB1 expression was increased in the nucleus and cytoplasm of striatal neurons; however, there was no SAFB1 staining in the striatal neurons of controls. In PD substantia nigra, we did not see any changes in neuronal SAFB1 expression. iCLIP analysis found that SAFB1 crosslink sites within ATXN1 RNA were adjacent to the start and within the glutamine repeat sequence. Further investigation found increased binding of SAFB1 to pathogenic ATXN1‐85Q mRNA. These novel data strongly suggest SAFB1 contributes to the etiology of SCA and Huntington’s chorea and that it may be a pathological marker of polyglutamine repeat expansion diseases.
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spelling pubmed-80181662021-06-28 Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea Buckner, Nicola Kemp, Kevin C. Scott, Helen L. Shi, Gongyu Rivers, Caroline Gialeli, Andriana Wong, Liang-Fong Cordero-LLana, Oscar Allen, Nicholas Wilkins, Alastair Uney, James B. Brain Pathol Research Articles SAFB1 is a DNA and RNA binding protein that is highly expressed in the cerebellum and hippocampus and is involved in the processing of coding and non‐coding RNAs, splicing and dendritic function. We analyzed SAFB1 expression in the post‐mortem brain tissue of spinocerebellar ataxia (SCA), Huntington’s disease (HD), Multiple sclerosis (MS), Parkinson’s disease patients and controls. In SCA cases, the expression of SAFB1 in the nucleus was increased and there was abnormal and extensive expression in the cytoplasm where it co‐localized with the markers of Purkinje cell injury. Significantly, no SAFB1 expression was found in the cerebellar neurons of the dentate nucleus in control or MS patients; however, in SCA patients, SAFB1 expression was increased significantly in both the nucleus and cytoplasm of dentate neurons. In HD, we found that SAFB1 expression was increased in the nucleus and cytoplasm of striatal neurons; however, there was no SAFB1 staining in the striatal neurons of controls. In PD substantia nigra, we did not see any changes in neuronal SAFB1 expression. iCLIP analysis found that SAFB1 crosslink sites within ATXN1 RNA were adjacent to the start and within the glutamine repeat sequence. Further investigation found increased binding of SAFB1 to pathogenic ATXN1‐85Q mRNA. These novel data strongly suggest SAFB1 contributes to the etiology of SCA and Huntington’s chorea and that it may be a pathological marker of polyglutamine repeat expansion diseases. John Wiley and Sons Inc. 2020-07-13 /pmc/articles/PMC8018166/ /pubmed/32580238 http://dx.doi.org/10.1111/bpa.12872 Text en © 2020 The Authors. Brain Pathology published by John Wiley & Sons Ltd on behalf of International Society of Neuropathology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Buckner, Nicola
Kemp, Kevin C.
Scott, Helen L.
Shi, Gongyu
Rivers, Caroline
Gialeli, Andriana
Wong, Liang-Fong
Cordero-LLana, Oscar
Allen, Nicholas
Wilkins, Alastair
Uney, James B.
Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
title Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
title_full Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
title_fullStr Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
title_full_unstemmed Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
title_short Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
title_sort abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and huntington’s chorea
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018166/
https://www.ncbi.nlm.nih.gov/pubmed/32580238
http://dx.doi.org/10.1111/bpa.12872
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