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Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
Human T-cell leukemia virus type 1 (HTLV-1) was the first oncogenic human retrovirus identified in humans which infects at least 10–15 million people worldwide. Large HTLV-1 endemic areas exist in Southern Japan, the Caribbean, Central and South America, the Middle East, Melanesia, and equatorial re...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018625/ https://www.ncbi.nlm.nih.gov/pubmed/33798232 http://dx.doi.org/10.1371/journal.pone.0248001 |
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author | Raza, Md. Thosif Mizan, Shagufta Yasmin, Farhana Akash, Al-Shahriar Shahik, Shah Md. |
author_facet | Raza, Md. Thosif Mizan, Shagufta Yasmin, Farhana Akash, Al-Shahriar Shahik, Shah Md. |
author_sort | Raza, Md. Thosif |
collection | PubMed |
description | Human T-cell leukemia virus type 1 (HTLV-1) was the first oncogenic human retrovirus identified in humans which infects at least 10–15 million people worldwide. Large HTLV-1 endemic areas exist in Southern Japan, the Caribbean, Central and South America, the Middle East, Melanesia, and equatorial regions of Africa. HTLV-1 TAX viral protein is thought to play a critical role in HTLV-1 associated diseases. We have used numerous bio-informatics and immuno-informatics implements comprising sequence and construction tools for the construction of a 3D model and epitope prediction for HTLV-1 Tax viral protein. The conformational linear B-cell and T-cell epitopes for HTLV-1 TAX viral protein have been predicted for their possible collective use as vaccine candidates. Based on in silico investigation two B cell epitopes, KEADDNDHEPQISPGGLEPPSEKHFR and DGTPMISGPCPKDGQPS spanning from 324–349 and 252–268 respectively; and T cell epitopes, LLFGYPVYV, ITWPLLPHV and GLLPFHSTL ranging from 11–19, 163–171 and 233–241 were found most antigenic and immunogenic epitopes. Among different vaccine constructs generated by different combinations of these epitopes our predicted vaccine construct was found to be most antigenic with a score of 0.57. T cell epitopes interacted strongly with HLA-A*0201 suggesting a significant immune response evoked by these epitopes. Molecular docking study also showed a high binding affinity of the vaccine construct for TLR4. The study was carried out to predict antigenic determinants of the Tax protein along with the 3D protein modeling. The study revealed a potential multi epitope vaccine that can raise the desired immune response against HTLV-1 and be useful in developing effective vaccines against Human T-lymphotropic virus. |
format | Online Article Text |
id | pubmed-8018625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80186252021-04-13 Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) Raza, Md. Thosif Mizan, Shagufta Yasmin, Farhana Akash, Al-Shahriar Shahik, Shah Md. PLoS One Research Article Human T-cell leukemia virus type 1 (HTLV-1) was the first oncogenic human retrovirus identified in humans which infects at least 10–15 million people worldwide. Large HTLV-1 endemic areas exist in Southern Japan, the Caribbean, Central and South America, the Middle East, Melanesia, and equatorial regions of Africa. HTLV-1 TAX viral protein is thought to play a critical role in HTLV-1 associated diseases. We have used numerous bio-informatics and immuno-informatics implements comprising sequence and construction tools for the construction of a 3D model and epitope prediction for HTLV-1 Tax viral protein. The conformational linear B-cell and T-cell epitopes for HTLV-1 TAX viral protein have been predicted for their possible collective use as vaccine candidates. Based on in silico investigation two B cell epitopes, KEADDNDHEPQISPGGLEPPSEKHFR and DGTPMISGPCPKDGQPS spanning from 324–349 and 252–268 respectively; and T cell epitopes, LLFGYPVYV, ITWPLLPHV and GLLPFHSTL ranging from 11–19, 163–171 and 233–241 were found most antigenic and immunogenic epitopes. Among different vaccine constructs generated by different combinations of these epitopes our predicted vaccine construct was found to be most antigenic with a score of 0.57. T cell epitopes interacted strongly with HLA-A*0201 suggesting a significant immune response evoked by these epitopes. Molecular docking study also showed a high binding affinity of the vaccine construct for TLR4. The study was carried out to predict antigenic determinants of the Tax protein along with the 3D protein modeling. The study revealed a potential multi epitope vaccine that can raise the desired immune response against HTLV-1 and be useful in developing effective vaccines against Human T-lymphotropic virus. Public Library of Science 2021-04-02 /pmc/articles/PMC8018625/ /pubmed/33798232 http://dx.doi.org/10.1371/journal.pone.0248001 Text en © 2021 Raza et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Raza, Md. Thosif Mizan, Shagufta Yasmin, Farhana Akash, Al-Shahriar Shahik, Shah Md. Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) |
title | Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) |
title_full | Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) |
title_fullStr | Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) |
title_full_unstemmed | Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) |
title_short | Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) |
title_sort | epitope-based universal vaccine for human t-lymphotropic virus-1 (htlv-1) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018625/ https://www.ncbi.nlm.nih.gov/pubmed/33798232 http://dx.doi.org/10.1371/journal.pone.0248001 |
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