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Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)

Human T-cell leukemia virus type 1 (HTLV-1) was the first oncogenic human retrovirus identified in humans which infects at least 10–15 million people worldwide. Large HTLV-1 endemic areas exist in Southern Japan, the Caribbean, Central and South America, the Middle East, Melanesia, and equatorial re...

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Autores principales: Raza, Md. Thosif, Mizan, Shagufta, Yasmin, Farhana, Akash, Al-Shahriar, Shahik, Shah Md.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018625/
https://www.ncbi.nlm.nih.gov/pubmed/33798232
http://dx.doi.org/10.1371/journal.pone.0248001
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author Raza, Md. Thosif
Mizan, Shagufta
Yasmin, Farhana
Akash, Al-Shahriar
Shahik, Shah Md.
author_facet Raza, Md. Thosif
Mizan, Shagufta
Yasmin, Farhana
Akash, Al-Shahriar
Shahik, Shah Md.
author_sort Raza, Md. Thosif
collection PubMed
description Human T-cell leukemia virus type 1 (HTLV-1) was the first oncogenic human retrovirus identified in humans which infects at least 10–15 million people worldwide. Large HTLV-1 endemic areas exist in Southern Japan, the Caribbean, Central and South America, the Middle East, Melanesia, and equatorial regions of Africa. HTLV-1 TAX viral protein is thought to play a critical role in HTLV-1 associated diseases. We have used numerous bio-informatics and immuno-informatics implements comprising sequence and construction tools for the construction of a 3D model and epitope prediction for HTLV-1 Tax viral protein. The conformational linear B-cell and T-cell epitopes for HTLV-1 TAX viral protein have been predicted for their possible collective use as vaccine candidates. Based on in silico investigation two B cell epitopes, KEADDNDHEPQISPGGLEPPSEKHFR and DGTPMISGPCPKDGQPS spanning from 324–349 and 252–268 respectively; and T cell epitopes, LLFGYPVYV, ITWPLLPHV and GLLPFHSTL ranging from 11–19, 163–171 and 233–241 were found most antigenic and immunogenic epitopes. Among different vaccine constructs generated by different combinations of these epitopes our predicted vaccine construct was found to be most antigenic with a score of 0.57. T cell epitopes interacted strongly with HLA-A*0201 suggesting a significant immune response evoked by these epitopes. Molecular docking study also showed a high binding affinity of the vaccine construct for TLR4. The study was carried out to predict antigenic determinants of the Tax protein along with the 3D protein modeling. The study revealed a potential multi epitope vaccine that can raise the desired immune response against HTLV-1 and be useful in developing effective vaccines against Human T-lymphotropic virus.
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spelling pubmed-80186252021-04-13 Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1) Raza, Md. Thosif Mizan, Shagufta Yasmin, Farhana Akash, Al-Shahriar Shahik, Shah Md. PLoS One Research Article Human T-cell leukemia virus type 1 (HTLV-1) was the first oncogenic human retrovirus identified in humans which infects at least 10–15 million people worldwide. Large HTLV-1 endemic areas exist in Southern Japan, the Caribbean, Central and South America, the Middle East, Melanesia, and equatorial regions of Africa. HTLV-1 TAX viral protein is thought to play a critical role in HTLV-1 associated diseases. We have used numerous bio-informatics and immuno-informatics implements comprising sequence and construction tools for the construction of a 3D model and epitope prediction for HTLV-1 Tax viral protein. The conformational linear B-cell and T-cell epitopes for HTLV-1 TAX viral protein have been predicted for their possible collective use as vaccine candidates. Based on in silico investigation two B cell epitopes, KEADDNDHEPQISPGGLEPPSEKHFR and DGTPMISGPCPKDGQPS spanning from 324–349 and 252–268 respectively; and T cell epitopes, LLFGYPVYV, ITWPLLPHV and GLLPFHSTL ranging from 11–19, 163–171 and 233–241 were found most antigenic and immunogenic epitopes. Among different vaccine constructs generated by different combinations of these epitopes our predicted vaccine construct was found to be most antigenic with a score of 0.57. T cell epitopes interacted strongly with HLA-A*0201 suggesting a significant immune response evoked by these epitopes. Molecular docking study also showed a high binding affinity of the vaccine construct for TLR4. The study was carried out to predict antigenic determinants of the Tax protein along with the 3D protein modeling. The study revealed a potential multi epitope vaccine that can raise the desired immune response against HTLV-1 and be useful in developing effective vaccines against Human T-lymphotropic virus. Public Library of Science 2021-04-02 /pmc/articles/PMC8018625/ /pubmed/33798232 http://dx.doi.org/10.1371/journal.pone.0248001 Text en © 2021 Raza et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Raza, Md. Thosif
Mizan, Shagufta
Yasmin, Farhana
Akash, Al-Shahriar
Shahik, Shah Md.
Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
title Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
title_full Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
title_fullStr Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
title_full_unstemmed Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
title_short Epitope-based universal vaccine for Human T-lymphotropic virus-1 (HTLV-1)
title_sort epitope-based universal vaccine for human t-lymphotropic virus-1 (htlv-1)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018625/
https://www.ncbi.nlm.nih.gov/pubmed/33798232
http://dx.doi.org/10.1371/journal.pone.0248001
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