Rapid screening for SARS-CoV-2 variants of concern in clinical and environmental samples using nested RT-PCR assays targeting key mutations of the spike protein

New SARS-CoV-2 mutations are constantly emerging, raising concerns of increased transmissibility, virulence or escape from host immune response. We describe a nested RT-PCR assay (~1500 bps) to detect multiple nucleotide changes resulting in key spike protein mutations distinctive of the major known...

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Detalles Bibliográficos
Autores principales: La Rosa, G, Mancini, P., Bonanno Ferraro, G., Veneri, C., Iaconelli, M., Lucentini, L., Bonadonna, L., Brusaferro, S., Brandtner, D., Fasanella, A., Pace, L., Parisi, A., Galante, D., Suffredini, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018700/
https://www.ncbi.nlm.nih.gov/pubmed/33857895
http://dx.doi.org/10.1016/j.watres.2021.117104

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