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Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents

Detection of early osteoarthritis to stabilize or reverse the damage to articular cartilage would improve patient function, reduce disability, and limit the need for joint replacement. In this study, we investigated nondestructive photon-processing spectral computed tomography (CT) for the quantitat...

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Autores principales: Baer, Kenzie, Kieser, Sandra, Schon, Ben, Rajendran, Kishore, ten Harkel, Timen, Ramyar, Mohsen, Löbker, Caroline, Bateman, Christopher, Butler, Anthony, Raja, Aamir, Hooper, Gary, Anderson, Nigel, Woodfield, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018795/
https://www.ncbi.nlm.nih.gov/pubmed/33834156
http://dx.doi.org/10.1063/5.0035312
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author Baer, Kenzie
Kieser, Sandra
Schon, Ben
Rajendran, Kishore
ten Harkel, Timen
Ramyar, Mohsen
Löbker, Caroline
Bateman, Christopher
Butler, Anthony
Raja, Aamir
Hooper, Gary
Anderson, Nigel
Woodfield, Tim
author_facet Baer, Kenzie
Kieser, Sandra
Schon, Ben
Rajendran, Kishore
ten Harkel, Timen
Ramyar, Mohsen
Löbker, Caroline
Bateman, Christopher
Butler, Anthony
Raja, Aamir
Hooper, Gary
Anderson, Nigel
Woodfield, Tim
author_sort Baer, Kenzie
collection PubMed
description Detection of early osteoarthritis to stabilize or reverse the damage to articular cartilage would improve patient function, reduce disability, and limit the need for joint replacement. In this study, we investigated nondestructive photon-processing spectral computed tomography (CT) for the quantitative measurement of the glycosaminoglycan (GAG) content compared to destructive histological and biochemical assay techniques in normal and osteoarthritic tissues. Cartilage-bone cores from healthy bovine stifles were incubated in 50% ioxaglate (Hexabrix(®)) or 100% gadobenate dimeglumine (MultiHance(®)). A photon-processing spectral CT (MARS) scanner with a CdTe-Medipix3RX detector imaged samples. Calibration phantoms of ioxaglate and gadobenate dimeglumine were used to determine iodine and gadolinium concentrations from photon-processing spectral CT images to correlate with the GAG content measured using a dimethylmethylene blue assay. The zonal distribution of GAG was compared between photon-processing spectral CT images and histological sections. Furthermore, discrimination and quantification of GAG in osteoarthritic human tibial plateau tissue using the same contrast agents were demonstrated. Contrast agent concentrations were inversely related to the GAG content. The GAG concentration increased from 25 μg/ml (85 mg/ml iodine or 43 mg/ml gadolinium) in the superficial layer to 75 μg/ml (65 mg/ml iodine or 37 mg/ml gadolinium) in the deep layer of healthy bovine cartilage. Deep zone articular cartilage could be distinguished from subchondral bone by utilizing the material decomposition technique. Photon-processing spectral CT images correlated with histological sections in healthy and osteoarthritic tissues. Post-imaging material decomposition was able to quantify the GAG content and distribution throughout healthy and osteoarthritic cartilage using Hexabrix(®) and MultiHance(®) while differentiating the underlying subchondral bone.
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spelling pubmed-80187952021-04-07 Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents Baer, Kenzie Kieser, Sandra Schon, Ben Rajendran, Kishore ten Harkel, Timen Ramyar, Mohsen Löbker, Caroline Bateman, Christopher Butler, Anthony Raja, Aamir Hooper, Gary Anderson, Nigel Woodfield, Tim APL Bioeng Articles Detection of early osteoarthritis to stabilize or reverse the damage to articular cartilage would improve patient function, reduce disability, and limit the need for joint replacement. In this study, we investigated nondestructive photon-processing spectral computed tomography (CT) for the quantitative measurement of the glycosaminoglycan (GAG) content compared to destructive histological and biochemical assay techniques in normal and osteoarthritic tissues. Cartilage-bone cores from healthy bovine stifles were incubated in 50% ioxaglate (Hexabrix(®)) or 100% gadobenate dimeglumine (MultiHance(®)). A photon-processing spectral CT (MARS) scanner with a CdTe-Medipix3RX detector imaged samples. Calibration phantoms of ioxaglate and gadobenate dimeglumine were used to determine iodine and gadolinium concentrations from photon-processing spectral CT images to correlate with the GAG content measured using a dimethylmethylene blue assay. The zonal distribution of GAG was compared between photon-processing spectral CT images and histological sections. Furthermore, discrimination and quantification of GAG in osteoarthritic human tibial plateau tissue using the same contrast agents were demonstrated. Contrast agent concentrations were inversely related to the GAG content. The GAG concentration increased from 25 μg/ml (85 mg/ml iodine or 43 mg/ml gadolinium) in the superficial layer to 75 μg/ml (65 mg/ml iodine or 37 mg/ml gadolinium) in the deep layer of healthy bovine cartilage. Deep zone articular cartilage could be distinguished from subchondral bone by utilizing the material decomposition technique. Photon-processing spectral CT images correlated with histological sections in healthy and osteoarthritic tissues. Post-imaging material decomposition was able to quantify the GAG content and distribution throughout healthy and osteoarthritic cartilage using Hexabrix(®) and MultiHance(®) while differentiating the underlying subchondral bone. AIP Publishing LLC 2021-04-01 /pmc/articles/PMC8018795/ /pubmed/33834156 http://dx.doi.org/10.1063/5.0035312 Text en © 2021 Author(s). 2473-2877/2021/5(2)/026101/10 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Baer, Kenzie
Kieser, Sandra
Schon, Ben
Rajendran, Kishore
ten Harkel, Timen
Ramyar, Mohsen
Löbker, Caroline
Bateman, Christopher
Butler, Anthony
Raja, Aamir
Hooper, Gary
Anderson, Nigel
Woodfield, Tim
Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
title Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
title_full Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
title_fullStr Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
title_full_unstemmed Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
title_short Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
title_sort spectral ct imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018795/
https://www.ncbi.nlm.nih.gov/pubmed/33834156
http://dx.doi.org/10.1063/5.0035312
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