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Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents
Detection of early osteoarthritis to stabilize or reverse the damage to articular cartilage would improve patient function, reduce disability, and limit the need for joint replacement. In this study, we investigated nondestructive photon-processing spectral computed tomography (CT) for the quantitat...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018795/ https://www.ncbi.nlm.nih.gov/pubmed/33834156 http://dx.doi.org/10.1063/5.0035312 |
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author | Baer, Kenzie Kieser, Sandra Schon, Ben Rajendran, Kishore ten Harkel, Timen Ramyar, Mohsen Löbker, Caroline Bateman, Christopher Butler, Anthony Raja, Aamir Hooper, Gary Anderson, Nigel Woodfield, Tim |
author_facet | Baer, Kenzie Kieser, Sandra Schon, Ben Rajendran, Kishore ten Harkel, Timen Ramyar, Mohsen Löbker, Caroline Bateman, Christopher Butler, Anthony Raja, Aamir Hooper, Gary Anderson, Nigel Woodfield, Tim |
author_sort | Baer, Kenzie |
collection | PubMed |
description | Detection of early osteoarthritis to stabilize or reverse the damage to articular cartilage would improve patient function, reduce disability, and limit the need for joint replacement. In this study, we investigated nondestructive photon-processing spectral computed tomography (CT) for the quantitative measurement of the glycosaminoglycan (GAG) content compared to destructive histological and biochemical assay techniques in normal and osteoarthritic tissues. Cartilage-bone cores from healthy bovine stifles were incubated in 50% ioxaglate (Hexabrix(®)) or 100% gadobenate dimeglumine (MultiHance(®)). A photon-processing spectral CT (MARS) scanner with a CdTe-Medipix3RX detector imaged samples. Calibration phantoms of ioxaglate and gadobenate dimeglumine were used to determine iodine and gadolinium concentrations from photon-processing spectral CT images to correlate with the GAG content measured using a dimethylmethylene blue assay. The zonal distribution of GAG was compared between photon-processing spectral CT images and histological sections. Furthermore, discrimination and quantification of GAG in osteoarthritic human tibial plateau tissue using the same contrast agents were demonstrated. Contrast agent concentrations were inversely related to the GAG content. The GAG concentration increased from 25 μg/ml (85 mg/ml iodine or 43 mg/ml gadolinium) in the superficial layer to 75 μg/ml (65 mg/ml iodine or 37 mg/ml gadolinium) in the deep layer of healthy bovine cartilage. Deep zone articular cartilage could be distinguished from subchondral bone by utilizing the material decomposition technique. Photon-processing spectral CT images correlated with histological sections in healthy and osteoarthritic tissues. Post-imaging material decomposition was able to quantify the GAG content and distribution throughout healthy and osteoarthritic cartilage using Hexabrix(®) and MultiHance(®) while differentiating the underlying subchondral bone. |
format | Online Article Text |
id | pubmed-8018795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-80187952021-04-07 Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents Baer, Kenzie Kieser, Sandra Schon, Ben Rajendran, Kishore ten Harkel, Timen Ramyar, Mohsen Löbker, Caroline Bateman, Christopher Butler, Anthony Raja, Aamir Hooper, Gary Anderson, Nigel Woodfield, Tim APL Bioeng Articles Detection of early osteoarthritis to stabilize or reverse the damage to articular cartilage would improve patient function, reduce disability, and limit the need for joint replacement. In this study, we investigated nondestructive photon-processing spectral computed tomography (CT) for the quantitative measurement of the glycosaminoglycan (GAG) content compared to destructive histological and biochemical assay techniques in normal and osteoarthritic tissues. Cartilage-bone cores from healthy bovine stifles were incubated in 50% ioxaglate (Hexabrix(®)) or 100% gadobenate dimeglumine (MultiHance(®)). A photon-processing spectral CT (MARS) scanner with a CdTe-Medipix3RX detector imaged samples. Calibration phantoms of ioxaglate and gadobenate dimeglumine were used to determine iodine and gadolinium concentrations from photon-processing spectral CT images to correlate with the GAG content measured using a dimethylmethylene blue assay. The zonal distribution of GAG was compared between photon-processing spectral CT images and histological sections. Furthermore, discrimination and quantification of GAG in osteoarthritic human tibial plateau tissue using the same contrast agents were demonstrated. Contrast agent concentrations were inversely related to the GAG content. The GAG concentration increased from 25 μg/ml (85 mg/ml iodine or 43 mg/ml gadolinium) in the superficial layer to 75 μg/ml (65 mg/ml iodine or 37 mg/ml gadolinium) in the deep layer of healthy bovine cartilage. Deep zone articular cartilage could be distinguished from subchondral bone by utilizing the material decomposition technique. Photon-processing spectral CT images correlated with histological sections in healthy and osteoarthritic tissues. Post-imaging material decomposition was able to quantify the GAG content and distribution throughout healthy and osteoarthritic cartilage using Hexabrix(®) and MultiHance(®) while differentiating the underlying subchondral bone. AIP Publishing LLC 2021-04-01 /pmc/articles/PMC8018795/ /pubmed/33834156 http://dx.doi.org/10.1063/5.0035312 Text en © 2021 Author(s). 2473-2877/2021/5(2)/026101/10 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Baer, Kenzie Kieser, Sandra Schon, Ben Rajendran, Kishore ten Harkel, Timen Ramyar, Mohsen Löbker, Caroline Bateman, Christopher Butler, Anthony Raja, Aamir Hooper, Gary Anderson, Nigel Woodfield, Tim Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
title | Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
title_full | Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
title_fullStr | Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
title_full_unstemmed | Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
title_short | Spectral CT imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
title_sort | spectral ct imaging of human osteoarthritic cartilage via quantitative assessment of glycosaminoglycan content using multiple contrast agents |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018795/ https://www.ncbi.nlm.nih.gov/pubmed/33834156 http://dx.doi.org/10.1063/5.0035312 |
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