A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2

After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and opt...

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Autores principales: Han, Xiaojian, Wang, Yingming, Li, Shenglong, Hu, Chao, Li, Tingting, Gu, Chenjian, Wang, Kai, Shen, Meiying, Wang, Jianwei, Hu, Jie, Wu, Ruixin, Mu, Song, Gong, Fang, Chen, Qian, Gao, Fengxia, Huang, Jingjing, Long, Yingyi, Luo, Feiyang, Song, Shuyi, Long, Shunhua, Hao, Yanan, Li, Luo, Wu, Yang, Xu, Wei, Cai, Xia, Gao, Qingzhu, Zhang, Guiji, He, Changlong, Deng, Kun, Du, Li, Nai, Yaru, Wang, Wang, Xie, Youhua, Qu, Di, Huang, Ailong, Tang, Ni, Jin, Aishun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019923/
https://www.ncbi.nlm.nih.gov/pubmed/33828563
http://dx.doi.org/10.3389/fimmu.2021.653189
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author Han, Xiaojian
Wang, Yingming
Li, Shenglong
Hu, Chao
Li, Tingting
Gu, Chenjian
Wang, Kai
Shen, Meiying
Wang, Jianwei
Hu, Jie
Wu, Ruixin
Mu, Song
Gong, Fang
Chen, Qian
Gao, Fengxia
Huang, Jingjing
Long, Yingyi
Luo, Feiyang
Song, Shuyi
Long, Shunhua
Hao, Yanan
Li, Luo
Wu, Yang
Xu, Wei
Cai, Xia
Gao, Qingzhu
Zhang, Guiji
He, Changlong
Deng, Kun
Du, Li
Nai, Yaru
Wang, Wang
Xie, Youhua
Qu, Di
Huang, Ailong
Tang, Ni
Jin, Aishun
author_facet Han, Xiaojian
Wang, Yingming
Li, Shenglong
Hu, Chao
Li, Tingting
Gu, Chenjian
Wang, Kai
Shen, Meiying
Wang, Jianwei
Hu, Jie
Wu, Ruixin
Mu, Song
Gong, Fang
Chen, Qian
Gao, Fengxia
Huang, Jingjing
Long, Yingyi
Luo, Feiyang
Song, Shuyi
Long, Shunhua
Hao, Yanan
Li, Luo
Wu, Yang
Xu, Wei
Cai, Xia
Gao, Qingzhu
Zhang, Guiji
He, Changlong
Deng, Kun
Du, Li
Nai, Yaru
Wang, Wang
Xie, Youhua
Qu, Di
Huang, Ailong
Tang, Ni
Jin, Aishun
author_sort Han, Xiaojian
collection PubMed
description After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and optimized a strategically screening method for NAbs, which has enabled us to obtain SARS-CoV-2 receptor-binding domain (RBD) specific NAbs within 6 days, followed by additional 9 days for antibody production and function analysis. Using this method, we obtained 198 specific Abs against SARS-CoV-2 RBD from the blood samples of COVID-19 convalescent patients, and 96 of them showed neutralizing activity. At least 20% of these NAbs exhibited advanced neutralizing potency and high affinity, with the top two NAbs showing half-maximal inhibitory concentration (IC(50)) to block authentic SARS-CoV-2 at 9.88 and 11.13 ng/ml, respectively. Altogether, our study provides an effective methodology with high applicable value for discovering potential preventative and therapeutic NAbs for the emerging infectious diseases.
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spelling pubmed-80199232021-04-06 A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2 Han, Xiaojian Wang, Yingming Li, Shenglong Hu, Chao Li, Tingting Gu, Chenjian Wang, Kai Shen, Meiying Wang, Jianwei Hu, Jie Wu, Ruixin Mu, Song Gong, Fang Chen, Qian Gao, Fengxia Huang, Jingjing Long, Yingyi Luo, Feiyang Song, Shuyi Long, Shunhua Hao, Yanan Li, Luo Wu, Yang Xu, Wei Cai, Xia Gao, Qingzhu Zhang, Guiji He, Changlong Deng, Kun Du, Li Nai, Yaru Wang, Wang Xie, Youhua Qu, Di Huang, Ailong Tang, Ni Jin, Aishun Front Immunol Immunology After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and optimized a strategically screening method for NAbs, which has enabled us to obtain SARS-CoV-2 receptor-binding domain (RBD) specific NAbs within 6 days, followed by additional 9 days for antibody production and function analysis. Using this method, we obtained 198 specific Abs against SARS-CoV-2 RBD from the blood samples of COVID-19 convalescent patients, and 96 of them showed neutralizing activity. At least 20% of these NAbs exhibited advanced neutralizing potency and high affinity, with the top two NAbs showing half-maximal inhibitory concentration (IC(50)) to block authentic SARS-CoV-2 at 9.88 and 11.13 ng/ml, respectively. Altogether, our study provides an effective methodology with high applicable value for discovering potential preventative and therapeutic NAbs for the emerging infectious diseases. Frontiers Media S.A. 2021-03-22 /pmc/articles/PMC8019923/ /pubmed/33828563 http://dx.doi.org/10.3389/fimmu.2021.653189 Text en Copyright © 2021 Han, Wang, Li, Hu, Li, Gu, Wang, Shen, Wang, Hu, Wu, Mu, Gong, Chen, Gao, Huang, Long, Luo, Song, Long, Hao, Li, Wu, Xu, Cai, Gao, Zhang, He, Deng, Du, Nai, Wang, Xie, Qu, Huang, Tang and Jin http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Han, Xiaojian
Wang, Yingming
Li, Shenglong
Hu, Chao
Li, Tingting
Gu, Chenjian
Wang, Kai
Shen, Meiying
Wang, Jianwei
Hu, Jie
Wu, Ruixin
Mu, Song
Gong, Fang
Chen, Qian
Gao, Fengxia
Huang, Jingjing
Long, Yingyi
Luo, Feiyang
Song, Shuyi
Long, Shunhua
Hao, Yanan
Li, Luo
Wu, Yang
Xu, Wei
Cai, Xia
Gao, Qingzhu
Zhang, Guiji
He, Changlong
Deng, Kun
Du, Li
Nai, Yaru
Wang, Wang
Xie, Youhua
Qu, Di
Huang, Ailong
Tang, Ni
Jin, Aishun
A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
title A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
title_full A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
title_fullStr A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
title_full_unstemmed A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
title_short A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
title_sort rapid and efficient screening system for neutralizing antibodies and its application for sars-cov-2
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019923/
https://www.ncbi.nlm.nih.gov/pubmed/33828563
http://dx.doi.org/10.3389/fimmu.2021.653189
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