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LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in Gastric Cancer
BACKGROUND AND OBJECTIVE: Gastric cancer (GC) is a common tumor malignancy with high incidence and poor prognosis. Laminin is an indispensable component of basement membrane and extracellular matrix, which is responsible for bridging the internal and external environment of cells and transmitting si...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020091/ https://www.ncbi.nlm.nih.gov/pubmed/33784890 http://dx.doi.org/10.1177/15330338211004944 |
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author | Ran, Tao Chen, ZhiJi Zhao, LiWen Ran, Wei Fan, JinYu Hong, SiYa Yang, ZhaoXia |
author_facet | Ran, Tao Chen, ZhiJi Zhao, LiWen Ran, Wei Fan, JinYu Hong, SiYa Yang, ZhaoXia |
author_sort | Ran, Tao |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: Gastric cancer (GC) is a common tumor malignancy with high incidence and poor prognosis. Laminin is an indispensable component of basement membrane and extracellular matrix, which is responsible for bridging the internal and external environment of cells and transmitting signals. This study mainly explored the association of the LAMB1 expression with clinicopathological characteristics and prognosis in gastric cancer. METHODS: The expression data and clinical information of gastric cancer patients were downloaded from The Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG). And we analyzed the relationship between LAMB1 expression and clinical characteristics through R. CIBERSORTx was used to calculate the absolute score of immune cells in gastric tumor tissues. Then COX proportional hazard models and Kaplan-Meier curves were performed to evaluate the role of LAMB1 and its influence on prognosis in gastric cancer patients. Finally, GO and KEGG analysis were applied for LAMB1-related genes in gastric cancer, and PPI network was constructed in Cytoscape software. RESULTS: In the TCGA cohort, patients with gastric cancer frequently generated LAMB1 gene copy number variation, but had little effect on mRNA expression. Both in the TCGA and ACRG cohorts, the mRNA expression of LAMB1 in gastric cancer tissues was higher than it in normal tissues. All patients were divided into high expression group and low expression group according to the median expression level of LAMB1. The elevated expression group obviously had more advanced cases and higher infiltration levels of M2 macrophages. COX proportional hazard models and Kaplan-Meier curves revealed that patients with enhanced expression of LAMB1 have a worse prognosis. GO/KEGG analysis showed that LAMB1-related genes were enriched in PI3K-Akt signaling pathway, focal adhesion, ECM-receptor interaction, etc. CONCLUSIONS: The high expression of LAMB1 in gastric cancer is related to the poor prognosis of patients, and it may be related to microenvironmental changes in tumors. |
format | Online Article Text |
id | pubmed-8020091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-80200912021-04-16 LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in Gastric Cancer Ran, Tao Chen, ZhiJi Zhao, LiWen Ran, Wei Fan, JinYu Hong, SiYa Yang, ZhaoXia Technol Cancer Res Treat Original Article BACKGROUND AND OBJECTIVE: Gastric cancer (GC) is a common tumor malignancy with high incidence and poor prognosis. Laminin is an indispensable component of basement membrane and extracellular matrix, which is responsible for bridging the internal and external environment of cells and transmitting signals. This study mainly explored the association of the LAMB1 expression with clinicopathological characteristics and prognosis in gastric cancer. METHODS: The expression data and clinical information of gastric cancer patients were downloaded from The Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG). And we analyzed the relationship between LAMB1 expression and clinical characteristics through R. CIBERSORTx was used to calculate the absolute score of immune cells in gastric tumor tissues. Then COX proportional hazard models and Kaplan-Meier curves were performed to evaluate the role of LAMB1 and its influence on prognosis in gastric cancer patients. Finally, GO and KEGG analysis were applied for LAMB1-related genes in gastric cancer, and PPI network was constructed in Cytoscape software. RESULTS: In the TCGA cohort, patients with gastric cancer frequently generated LAMB1 gene copy number variation, but had little effect on mRNA expression. Both in the TCGA and ACRG cohorts, the mRNA expression of LAMB1 in gastric cancer tissues was higher than it in normal tissues. All patients were divided into high expression group and low expression group according to the median expression level of LAMB1. The elevated expression group obviously had more advanced cases and higher infiltration levels of M2 macrophages. COX proportional hazard models and Kaplan-Meier curves revealed that patients with enhanced expression of LAMB1 have a worse prognosis. GO/KEGG analysis showed that LAMB1-related genes were enriched in PI3K-Akt signaling pathway, focal adhesion, ECM-receptor interaction, etc. CONCLUSIONS: The high expression of LAMB1 in gastric cancer is related to the poor prognosis of patients, and it may be related to microenvironmental changes in tumors. SAGE Publications 2021-03-31 /pmc/articles/PMC8020091/ /pubmed/33784890 http://dx.doi.org/10.1177/15330338211004944 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Ran, Tao Chen, ZhiJi Zhao, LiWen Ran, Wei Fan, JinYu Hong, SiYa Yang, ZhaoXia LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in Gastric Cancer |
title | LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in
Gastric Cancer |
title_full | LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in
Gastric Cancer |
title_fullStr | LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in
Gastric Cancer |
title_full_unstemmed | LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in
Gastric Cancer |
title_short | LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in
Gastric Cancer |
title_sort | lamb1 is related to the t stage and indicates poor prognosis in
gastric cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020091/ https://www.ncbi.nlm.nih.gov/pubmed/33784890 http://dx.doi.org/10.1177/15330338211004944 |
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